Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TRIM8 Double Nickase Plasmid (h): sc-404916-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRIM8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TRIM8 Double Nickase Plasmid (h) and TRIM8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TRIM8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRIM8 Antibody (B-3): sc-398878
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRIM8 Double Nickase Plasmid (h) | sc-404916-NIC | 20 µg | $410.00 | |||
TRIM8 Double Nickase Plasmid (h2) | sc-404916-NIC-2 | 20 µg | $410.00 |
TRIM8 (tripartite motif containing 8) encodes a RING-type E3 ubiquitin ligase that regulates protein turnover and signaling amplitude in innate immunity and stress response pathways. TRIM8 has been linked to modulation of NF-κB and interferon-associated signaling and can influence transcriptional programs by ubiquitin-dependent control of pathway components and nuclear factors. In the nucleus and cytoplasm, TRIM8 contributes to protein quality control and context-dependent regulation of cell-cycle and apoptosis-related responses. Altered TRIM8 expression or function has been associated with neurodevelopmental and renal phenotypes, supporting its relevance for mechanistic studies of ubiquitin signaling and inflammatory regulation.
TRIM8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TRIM8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TRIM8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TRIM8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TRIM8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.