Date published: 2025-9-7
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TRAP150 Antibody (F-10) - Western Blotting - Image 374850
TRAP150 抗体 (F-10): sc-133250. K-562 (A), HL-60 (B) 和 HeLa (C) 全细胞裂解液中 TRAP150 表达的 Western 印迹分析.
TRAP150 抗体 (F-10): sc-133250. K-562 (A), HL-60 (B) 和 HeLa (C) 全细胞裂解液中 TRAP150 表达的 Western 印迹分析.

TRAP150 抗体 (F-10): sc-133250

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说明书
  • TRAP150 抗体 F-10 是小鼠单克隆 IgG1 κ，TRAP150抗体, 在4篇文献中引用，规格为200 µg/ml
  • 针对氨基酸191-490的图谱，在TRAP150的内部区域，human起源
  • TRAP150 抗体 (F-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 TRAP150
  • 抗TRAP150抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-133250 X, 200 µg/0.1 ml)
  • 关于如何获取TRAP150 (F-10): sc-133250的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 TRAP150 抗体 (F-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TRAP150 抗体 (F-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Thyroid Hormone Receptor Associated Protein抗体，包括TRAP150, TRAP220, TRAP230, TRAP240和TRAP95

TRAP150抗体（F-10）是一种IgG1 κ小鼠单克隆TRAP150抗体（也称为TRAP150抗体），可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（石蜡）和酶联免疫吸附试验检测小鼠、大鼠和人类来源的TRAP150蛋白。TRAP150抗体（F-10）既有非偶联形式的抗TRAP150抗体，也有多种偶联形式的抗TRAP150抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在哺乳动物细胞中，转录部分受高分子量共激活复合物的调节，这些复合物介导转录激活剂和起始因子之间的信号传导。这些复合物包括甲状腺激素受体相关蛋白(TRAP)复合物，该复合物与甲状腺激素受体(TR)、维生素D受体和其他类固醇受体相互作用，以促进激素诱导的转录激活。TRAP复合物由大小不等的多种蛋白质组成，包括TRAP95、TRAP100、TRAP150、TRAP220和TRAP230，其特征在于存在核受体识别基序，该基序介导TRAP蛋白以配体依赖性方式与核受体的结合。TRAP220和TRAP100广泛表达，在骨骼肌、心脏和胎盘中检测到的含量最为丰富。TRAP95、TRAP150和TRAP230通过与额外的转录共激活复合物SMCC（SRB和MED蛋白辅因子复合物）结合，促进TR诱导的转录，SMCC由各种亚基组成，这些亚基与酵母转录介体复合物的几个组分具有同源性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TRAP150 抗体 (F-10) 参考文献:

  1. 参与转录调控的新型人类 SRB/MED 辅助因子复合物 SMCC。  |  Gu, W., et al. 1999. Mol Cell. 3: 97-108. PMID: 10024883
  2. 甲状腺激素受体相关蛋白（TRAP）220 与转录中介因子（TIF）2 之间为结合核受体而进行的竞争。对 TRAP 和 p160 辅激活剂复合物招募的影响。  |  Treuter, E., et al. 1999. J Biol Chem. 274: 6667-77. PMID: 10037764
  3. TRAP 和 SMCC 复合物之间的同一性表明，核受体和各种哺乳动物活化剂的功能有了新的途径。  |  Ito, M., et al. 1999. Mol Cell. 3: 361-70. PMID: 10198638
  4. 鉴定小鼠 TRAP100：甲状腺激素和维生素 D 受体的转录核心调节因子。  |  Zhang, J. and Fondell, JD. 1999. Mol Endocrinol. 13: 1130-40. PMID: 10406464
  5. 核荷尔蒙受体的结构。  |  Kumar, R. and Thompson, EB. 1999. Steroids. 64: 310-9. PMID: 10406480
  6. TRAP/Mediator复合物的TRAP100成分在广泛的转录事件和发育中至关重要。  |  Ito, M., et al. 2002. EMBO J. 21: 3464-75. PMID: 12093747
  7. 转录共激活因子PBP（过氧化物酶体增殖物激活受体（PPAR）结合蛋白）是肝脏中PPARα调节基因表达所必需的。  |  Jia, Y., et al. 2004. J Biol Chem. 279: 24427-34. PMID: 15150259
  8. 动脉瘤性骨囊肿变异易位通过与 ZNF9, COL1A1, TRAP150 和 OMD 基因的启动子交换上调 USP6 的转录。  |  Oliveira, AM., et al. 2005. Oncogene. 24: 3419-26. PMID: 15735689
  9. 体外剪接的人mRNPs的蛋白质组成以及mRNP蛋白招募的差异要求。  |  Merz, C., et al. 2007. RNA. 13: 116-28. PMID: 17095540
  10. 通过下一代测序技术，在动脉瘤性骨囊肿中发现了基因融合PAFAH1B1-USP6和RUNX2-USP6。  |  Warren, M., et al. 2017. Cancer Genet. 212-213: 13-18. PMID: 28449806
  11. 甲状腺激素受体相关蛋白（TRAP）辅激活剂复合物的 TRAP220 成分以配体依赖的方式与核受体直接相互作用。  |  Yuan, CX., et al. 1998. Proc Natl Acad Sci U S A. 95: 7939-44. PMID: 9653119
  12. 哺乳动物转录调控介质及其作为信号转导途径终点的可能作用。  |  Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713

订购信息

产品名称产品编号规格价格数量收藏夹

TRAP150 抗体 (F-10)

sc-133250
200 µg/ml
$316.00

TRAP150 (F-10): m-IgG Fc BP-HRP 套装

sc-528782
200 µg Ab; 10 µg BP
$354.00

TRAP150 (F-10): m-IgGκ BP-HRP 套装

sc-521289
200 µg Ab, 40 µg BP
$354.00

TRAP150 (F-10): m-IgG1 BP-HRP 套装

sc-543126
200 µg Ab; 20 µg BP
$354.00

TRAP150 抗体 (F-10) X

sc-133250 X
200 µg/0.1 ml
$316.00

TRAP150 抗体 (F-10) AC

sc-133250 AC
500 µg/ml, 25% agarose
$416.00

TRAP150 抗体 (F-10) HRP

sc-133250 HRP
200 µg/ml
$316.00

TRAP150 抗体 (F-10) FITC

sc-133250 FITC
200 µg/ml
$330.00

TRAP150 抗体 (F-10) PE

sc-133250 PE
200 µg/ml
$343.00

TRAP150 抗体 (F-10) Alexa Fluor® 488

sc-133250 AF488
200 µg/ml
$357.00

TRAP150 抗体 (F-10) Alexa Fluor® 546

sc-133250 AF546
200 µg/ml
$357.00

TRAP150 抗体 (F-10) Alexa Fluor® 594

sc-133250 AF594
200 µg/ml
$357.00

TRAP150 抗体 (F-10) Alexa Fluor® 647

sc-133250 AF647
200 µg/ml
$357.00

TRAP150 抗体 (F-10) Alexa Fluor® 680

sc-133250 AF680
200 µg/ml
$357.00

TRAP150 抗体 (F-10) Alexa Fluor® 790

sc-133250 AF790
200 µg/ml
$357.00

Hello I am interested in the antibody against Thrap3 sc-133250 X. Since I am planning on using it in immunofluorescence on frozen sections as well as on ChIP, I wondered if you would recommend to purchase sc-133250 X for both applications? Many thanks

Asked by: Roberta M
Thank you for your question. The only difference between the conventional antibody product (sc-133250), and the TransCruz reagent (sc-133250 X), is that the TransCruz reagent is provided 10X more concentrated as 200 ug in 0.1 ml, in a buffer without any gelatin (as a stabilizing protein). If you would like to use the antibody for both immunofluorescence and ChIP then we would recommend purchasing the TransCruz reagent (sc-133250 X) because it could be used in either application. Please adjust your dilution(s) according to the more concentrated antibody. In addition, please note that this antibody has not yet been ChIP validated. The TransCruz primary antibody is recommended and warrantied for ChIP, however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-03

When should the TransCruz reagent sc-133250 X be used instead of the standard form sc-133250?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2019-01-03
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Rated 1 out of 5 by from Works for human Thrap3, not mouse Thrap3The antibody recognized Thrap3 protein from HeLa and SUDHL10 whole cell lysates, but not lysates from mouse CH12 or mouse embryonic stem cells. Thus, the antibody seems to only recognize human Thrap3 protein and not mouse Thrap3 protein.
Date published: 2023-04-18
Rated 1 out of 5 by from Didn't workAntibody produced no bands on a western blot using neonatal rat ventricular myocytes.
Date published: 2017-10-04
Rated 5 out of 5 by from Robust signal in western blot analysis ofRobust signal in western blot analysis of TRAP150 expression in K-562 nuclear extract. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Superb immunofluorescence nuclear stainingSuperb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-06
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TRAP150 抗体 (F-10) is rated 3.0 out of 5 by 4.
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