Date published: 2025-9-6

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transgelin-2 抗体 (C-12): sc-373928

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说明书
  • transgelin-2 抗体 C-12 是小鼠单克隆 IgG1 κ,transgelin-2抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列23-62位于human物种的transgelin-2的N-terminus附近
  • transgelin-2 抗体 (C-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 transgelin-2
  • 抗transgelin-2抗体(C-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取transgelin-2 (C-12): sc-373928免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是transgelin-2 Antibody (C-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与transgelin-2 Antibody (C-12) 打包提供(请参阅下面的订购信息)。

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関連項目

transgelin-2抗体(C-12)是一种IgG1 κ小鼠单克隆transgelin-2抗体(也称为TAGLN2抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的transgelin-2蛋白。transgelin-2抗体(C-12)既可以非偶联抗transgelin-2抗体形式提供,也可以多种偶联抗transgelin-2抗体形式提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。转胶蛋白(也称为SM22α)在平滑肌细胞中大量表达。转胶蛋白-2(也称为SM22α同源物)是转胶蛋白的同源物,也在平滑肌细胞和腹膜B-1细胞中表达。人类转胶蛋白-2基因(指定为TAGLN2)位于染色体1q231-q25上,编码一个包含钙调蛋白样重复序列和钙调蛋白同源(CH)结构域的199个氨基酸的蛋白质。转胶蛋白-2的功能可能与转胶蛋白非常相似。在胚胎发育过程中,转胶蛋白在平滑肌、心肌和骨骼肌中表达,但在晚期胎儿发育和成年期仅限于所有血管和内脏平滑肌细胞,并且在心脏中表达水平较低。转胶蛋白在几种转化细胞系中下调,表明转胶蛋白表达的减少可能是转化开始的早期指标。转胶蛋白还与肌动蛋白结合,导致肌动蛋白纤维在结合后几分钟内凝胶化。转胶蛋白与肌动蛋白的结合以1:6的肌动蛋白单体比例发生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

transgelin-2 抗体 (C-12) 参考文献:

  1. 丙戊酸致畸性:毒物基因组学方法。  |  Kultima, K., et al. 2004. Environ Health Perspect. 112: 1225-35. PMID: 15345369
  2. 肝细胞癌中肿瘤相关抗原的鉴定和分析。  |  Shi, YY., et al. 2005. Br J Cancer. 92: 929-34. PMID: 15756260
  3. B-1 细胞表达转胰蛋白酶 2:通过对腹膜 B-1 细胞进行蛋白质组分析,发现淋巴细胞意外表达一种平滑肌蛋白。  |  Francés, R., et al. 2006. Mol Immunol. 43: 2124-9. PMID: 16487589
  4. Transgelin 2 通过直接与 PTEN 相互作用并激活 PI3K/Akt/GSK-3β通路,促进乳腺癌的紫杉醇抗性、迁移和侵袭。  |  Liu, L., et al. 2019. Mol Cancer Ther. 18: 2457-2468. PMID: 31488699
  5. Transgelin-2和动力蛋白LIC2亚基的磷酸化调节控制着有丝分裂纺锤体的定向。  |  Sharma, A., et al. 2020. J Cell Sci. 133: PMID: 32467330
  6. 转胰蛋白酶-2参与丹参酮-IIA诱导的结直肠癌细胞凋亡  |  Zhang, Y., et al. 2022. Anal Cell Pathol (Amst). 2022: 9358583. PMID: 36204303
  7. 牛主动脉中一种新的 25 kDa 肌动蛋白结合蛋白的纯化, 表征和部分序列分析:SM22 同源物。  |  Kobayashi, R., et al. 1994. Biochem Biophys Res Commun. 198: 1275-80. PMID: 8117285
  8. Transgelin 的纯化和特性:一种对转化和形状变化敏感的肌动蛋白胶凝蛋白。  |  Shapland, C., et al. 1993. J Cell Biol. 121: 1065-73. PMID: 8501116
  9. 血清反应因子介导的调控网络管理平滑肌, 骨骼肌和心肌细胞中 SM22alpha 的转录。  |  Li, L., et al. 1997. Dev Biol. 187: 311-21. PMID: 9242426
  10. 成纤维细胞转球蛋白和平滑肌 SM22alpha 是同一种蛋白质,在许多细胞系中表达下调。  |  Lawson, D., et al. 1997. Cell Motil Cytoskeleton. 38: 250-7. PMID: 9384215
  11. 人类 SM22 基因(TAGLN)的表达和细胞遗传定位。  |  Camoretti-Mercado, B., et al. 1998. Genomics. 49: 452-7. PMID: 9615232
  12. 同源的 sm22alpha(Tagln)基因映射到小鼠的 1 号和 9 号染色体上:同源关系的进一步证据。  |  Stanier, P., et al. 1998. Genomics. 51: 144-7. PMID: 9693045

订购信息

产品名称产品编号规格价格数量收藏夹

transgelin-2 抗体 (C-12)

sc-373928
200 µg/ml
$316.00

transgelin-2 (C-12): m-IgG Fc BP-HRP 套装

sc-529613
200 µg Ab; 10 µg BP
$354.00

transgelin-2 (C-12): m-IgGκ BP-HRP 套装

sc-522560
200 µg Ab, 40 µg BP
$354.00

transgelin-2 (C-12): m-IgG1 BP-HRP 套装

sc-543605
200 µg Ab; 20 µg BP
$354.00

transgelin-2 抗体 (C-12) AC

sc-373928 AC
500 µg/ml, 25% agarose
$416.00

transgelin-2 抗体 (C-12) HRP

sc-373928 HRP
200 µg/ml
$316.00

transgelin-2 抗体 (C-12) FITC

sc-373928 FITC
200 µg/ml
$330.00

transgelin-2 抗体 (C-12) PE

sc-373928 PE
200 µg/ml
$343.00

transgelin-2 抗体 (C-12) Alexa Fluor® 488

sc-373928 AF488
200 µg/ml
$357.00

transgelin-2 抗体 (C-12) Alexa Fluor® 546

sc-373928 AF546
200 µg/ml
$357.00

transgelin-2 抗体 (C-12) Alexa Fluor® 594

sc-373928 AF594
200 µg/ml
$357.00

transgelin-2 抗体 (C-12) Alexa Fluor® 647

sc-373928 AF647
200 µg/ml
$357.00

transgelin-2 抗体 (C-12) Alexa Fluor® 680

sc-373928 AF680
200 µg/ml
$357.00

transgelin-2 抗体 (C-12) Alexa Fluor® 790

sc-373928 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with transgelin-2 (C-12): sc-373928 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good for WBA band around the predicted molecular weight size was identified in Huh1, HEK293 and mouse liver samples.
Date published: 2022-04-18
Rated 5 out of 5 by from Great antibody for immunohistochemical stainingVery nice immunohistochemical staining for mouse aortic tissues!
Date published: 2017-12-15
Rated 5 out of 5 by from Clean results on human aortic smooth smuscle cellsVery good antibody. Used for western blot on human primary smooth muscle cell lysates. Obtained a very clean band at the expected molecular weight.
Date published: 2017-10-23
Rated 5 out of 5 by from Great Antibody for IFThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-09
Rated 5 out of 5 by from Robust positive signal observed in WB in ARobust positive signal observed in WB in A-10 and WI 38 whole cell lysates. -SCBT QC
Date published: 2015-06-30
Rated 5 out of 5 by from Good specificity and signal strength in HISMGood specificity and signal strength in HISM whole cell lysate and mouse spleen tissue extract. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Produced superb immunofluorescence membraneProduced superb immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-18
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transgelin-2 抗体 (C-12) is rated 5.0 out of 5 by 7.
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