Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TOM40 CRISPR/Cas9 KO Plasmid (h): sc-400967
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TOM40 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TOM40 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tom40 Antibody (D-2): sc-365467
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TOM40 CRISPR/Cas9 KO Plasmid (h) | sc-400967 | 20 µg | $397.00 |
Overview
TOMM40 encodes TOM40, the pore-forming β-barrel subunit of the translocase of the outer mitochondrial membrane (TOM) complex that mediates recognition and import of nuclear-encoded mitochondrial proteins. By controlling precursor translocation into mitochondria, TOM40 supports oxidative phosphorylation, mitochondrial proteostasis, and stress-responsive quality control pathways including mitophagy. Altered TOMM40 function or expression can perturb mitochondrial bioenergetics and promote accumulation of mislocalized proteins, linking mitochondrial import defects to neurodegenerative and metabolic disease mechanisms. Genetic variation in the TOMM40 locus has been studied in relation to aging-associated phenotypes and neurological disease risk, motivating mechanistic investigation in relevant cellular models.
TOM40 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TOMM40 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TOMM40 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TOMM40 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TOM40 protein expression.
This CRISPR knockout system enables efficient generation of TOMM40-deficient cell models for investigation of TOM40 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TOMM40 exon(s) critical for TOM40 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TOMM40 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TOM40 CRISPR/Cas9 KO Plasmid (h) and TOM40 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TOMM40 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TOM40 HDR Plasmid (h) and TOM40 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TOMM40 homology arms to support homology-directed repair at defined TOMM40 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.