Date published: 2025-9-5

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TNF alpha 抗体 (TN3-19.12): sc-12744

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说明书
  • TNF alpha抗体(TN3-19.12)是一种亚美尼亚仓鼠单克隆 IgG TNF alpha抗体,被引用于45篇文献,发布于200 µg/ml
  • 针对mouse来源的纯化重组TNFα
  • TNF alpha 抗体 (TN3-19.12) 推荐用于 WB, IP 和 IF,检测mouse, rat 和rabbit 来源的 TNFα
  • 抗TNF alpha抗体(TN3-19.12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物的中和,sc-12744 L,200 µg/0.1 ml
  • 关于如何获取TNF alpha (TN3-19.12): sc-12744免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成TNF alpha 抗体 (TN3-19.12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

TNFα抗体(TN3-19.12)是一种仓鼠单克隆IgG抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和兔源的TNFα。TNFα抗体(TN3-19.12)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。TNFα,也称为cachectin,是一种参与全身炎症的关键细胞因子,由多种免疫细胞产生,包括淋巴细胞、中性粒细胞和巨噬细胞。TNFα可进行翻译后修饰,例如糖基化,从而影响其稳定性、活性和与受体的相互作用。这些修饰对于TNFα的正常功能至关重要,因为修饰会影响与TNF受体的结合亲和力,并调节下游信号通路,从而调节免疫反应和炎症。了解这些修饰对于制定针对TNFα的治疗策略至关重要,因为TNFα失调会导致包括自身免疫性疾病和癌症在内的各种疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TNF alpha 抗体 (TN3-19.12) 参考文献:

  1. 肿瘤坏死因子α(TNF-α)、抗肿瘤坏死因子α和脱髓鞘再论:一个正在发生的故事  |  Caminero, A., et al. 2011. J Neuroimmunol. 234: 1-6. PMID: 21474190
  2. 肿瘤坏死因子-α(TNF-α)在乳腺癌中的双重作用:分子见解和治疗方法。  |  Cruceriu, D., et al. 2020. Cell Oncol (Dordr). 43: 1-18. PMID: 31900901
  3. -肿瘤坏死因子α(TNF-α)基因的-308G/A多态性与代谢综合征的易感性:一项荟萃分析  |  Wang, D., et al. 2021. Sci Rep. 11: 3840. PMID: 33589701
  4. 肿瘤坏死因子α(TNF-α)在自身免疫性疾病中的作用以及目前TNF-α抑制剂在治疗中的应用。  |  Jang, DI., et al. 2021. Int J Mol Sci. 22: PMID: 33800290
  5. miR-146a与稳定型冠状动脉疾病中肿瘤坏死因子α(TNF-α)的关系  |  Pereira-da-Silva, T., et al. 2021. Medicina (Kaunas). 57: PMID: 34199767
  6. 肿瘤坏死因子-α(TNF-α)多态性在胃癌中的作用:一项 Meta 分析。  |  Gholamalizadeh, M., et al. 2022. J Gastrointest Cancer. 53: 756-769. PMID: 34478034
  7. 肿瘤坏死因子α(TNF-α)阻断可减轻大鼠口腔溃疡的急性炎症和延迟伤口愈合。  |  Freitas, MO., et al. 2022. Inflammopharmacology. 30: 1781-1798. PMID: 35948810
  8. 肿瘤坏死因子-α(TNF-α)在COVID-19中的前瞻性作用:预后、治疗和管理。  |  Mohd Zawawi, Z., et al. 2023. Int J Mol Sci. 24: PMID: 37047115
  9. 悖论性肿瘤坏死因子-α(TNF-α)抑制剂诱发的银屑病: 关于发病机制、临床表现和治疗的系统综述》。  |  Chokshi, A., et al. 2023. Cureus. 15: e42791. PMID: 37664349
  10. 脑脊髓液中脑型疟疾患儿肿瘤坏死因子-α(TNF-α)的水平。  |  Prasad, R., et al. 2023. J Trop Pediatr. 69: PMID: 37805828

订购信息

产品名称产品编号规格价格数量收藏夹

TNF alpha 抗体 (TN3-19.12)

sc-12744
200 µg/ml
$316.00

TNF alpha 抗体 (TN3-19.12) L

sc-12744 L
200 µg/0.1 ml
$316.00

TNF alpha 抗体 (TN3-19.12) AC

sc-12744 AC
500 µg/ml, 25% agarose
$416.00

TNF alpha 抗体 (TN3-19.12) HRP

sc-12744 HRP
200 µg/ml
$316.00

TNF alpha 抗体 (TN3-19.12) FITC

sc-12744 FITC
200 µg/ml
$330.00

TNF alpha 抗体 (TN3-19.12) PE

sc-12744 PE
200 µg/ml
$343.00

TNF alpha 抗体 (TN3-19.12) Alexa Fluor® 488

sc-12744 AF488
200 µg/ml
$357.00

TNF alpha 抗体 (TN3-19.12) Alexa Fluor® 546

sc-12744 AF546
200 µg/ml
$357.00

TNF alpha 抗体 (TN3-19.12) Alexa Fluor® 594

sc-12744 AF594
200 µg/ml
$357.00

TNF alpha 抗体 (TN3-19.12) Alexa Fluor® 647

sc-12744 AF647
200 µg/ml
$357.00

TNF alpha 抗体 (TN3-19.12) Alexa Fluor® 680

sc-12744 AF680
200 µg/ml
$357.00

TNF alpha 抗体 (TN3-19.12) Alexa Fluor® 790

sc-12744 AF790
200 µg/ml
$357.00

A manuscript reviewer requested we provide validation for this antibody. Do you have any internal data for IF?

Asked by: bawatts
Thank you for your question. We do not currently have IF data available for this antibody. The most current source of data for our antibodies is our website, so please check back for updates! There are multiple product citations using this antibody for IF or IHC applications. These include PMID's: 30911021 and 27681360.
Answered by: Technical Support
Date published: 2020-05-05

Can I use horse-anti mouse secondary antibody on this product (hamster) as the DAB kit we're using does not provide horse-anti hamster secondary antibody

Asked by: mnopqr
Thank you for your question. This is an Armenian hamster monoclonal IgG, and we recommend using anti-Armenian hamster IgG secondary antibody for detection. We do not know if anti-mouse IgG secondary antibody would be suitable for detection.
Answered by: Technical Support
Date published: 2019-05-13

can i get two band using this antibody , and i didnot get a clear band between 15-25, have you ever get two bands ?

Asked by: jiapangpang1993
Thank you for your product review/feedback. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge. Please contact your corresponding Technical Service department: https://www.scbt.com/scbt/resources/technical-service
Answered by: Technical Support Europe
Date published: 2017-10-23

Can TNFα (TN3-19.12): sc-12744 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TNFα (TN3-19.12): sc-12744 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from well function in WBGood results when tested TNF alpha in western blot of whole cell extracts from rat articular chondrocytes.
Date published: 2023-12-21
Rated 4 out of 5 by from good antibodyantibody was used in 1:500 dilution in mice liver lysates. Results were in general good, the tricky point will be the secondary antibody.
Date published: 2018-09-05
Rated 5 out of 5 by from Great feutureExcellent specificity! very good bands for western blot.
Date published: 2017-11-03
Rated 4 out of 5 by from Great antibodyI used it for western blotting at 1:1000 dilution. The signal was great.
Date published: 2017-10-26
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 3 out of 5 by from 抗体效果一般年初,申请了该抗体的单克隆试用装,效果一般。用的组织标本做的WB,条带微弱,可能本身表达量不高,上样量需要加倍。
Date published: 2017-09-25
Rated 5 out of 5 by from TNF效果评价大概八月份的时候,和其他的几个基因一起进行申请,效果还不错,值得去使用和购买,毕竟是大公司的产品,质量有保证
Date published: 2017-09-20
Rated 3 out of 5 by from 一般用于人脐静脉内皮细胞来源的蛋白进行WB检测,背景比较深,条带弱,不够清晰,可能后面还需要再优化下条件
Date published: 2017-08-07
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TNF alpha 抗体 (TN3-19.12) is rated 4.3 out of 5 by 20.
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