Date published: 2025-9-6
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TNF alpha Antibody (52B83) - Western Blotting - Image 58216
TNFα (52B83): sc-52746. 对 HeLa 全细胞裂解液中 TNFα 的表达进行 Western 印迹分析.
TNF alpha Antibody (52B83) - Western Blotting - Image 58216
TNFα (52B83): sc-52746. 对 HeLa 全细胞裂解液中 TNFα 的表达进行 Western 印迹分析.
TNF alpha Antibody (52B83) - Western Blotting - Image 15541
TNFα (52B83): sc-52746. 人重组 TNFα 的 Western 印迹分析.
TNFα (52B83): sc-52746. 对 HeLa 全细胞裂解液中 TNFα 的表达进行 Western 印迹分析.

TNF alpha 抗体 (52B83): sc-52746

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寫評論提問

说明书
  • TNF alpha抗体(52B83)是小鼠单克隆IgG1 κ, 在587篇文献中引用，规格为100 µg/ml
  • 与纯化的全长天然 TNFα human 起源
  • 推荐用于 mouse, rat 和 human 来源的natural and recombinant TNFα WB, IP, IF, IHC(P), FCM 和 ELISA检测; 也和以下物种反应，包括： 和 Guinea pig, Chimpanzee, Cynomolgus and Rhesus monkey; 不会与 TNFβ or lymphotoxin产生交联反应
  • 看 TNF alpha (C-4): sc-133192，可查看有标记的 TNF alpha 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取TNF alpha (52B83): sc-52746的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 TNF alpha 抗体 (52B83) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TNF alpha 抗体 (52B83) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Tumor Necrosis Factor抗体，包括TNF β

TNFα 抗体 (52B83) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过各种应用检测小鼠、大鼠和人类样本中的 TNFα，包括免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、石蜡包埋切片免疫组织化学 (IHCP)、流式细胞术 (FCM) 和酶联免疫吸附试验 (ELISA)。TNFα (52B83) 抗体为非结合型，可用于多种实验。肿瘤坏死因子α（TNFα）又称癌细胞凝集素，是一种重要的多向性细胞因子，在介导免疫反应和炎症中发挥着重要作用。TNFα 主要由淋巴细胞、中性粒细胞和巨噬细胞等免疫细胞产生，是调节各种生理过程（包括细胞凋亡、细胞增殖和分化）所必需的。TNFα 与靶细胞上的 TNF 受体相互作用，导致一连串的信号传导事件，从而调节免疫反应并促进炎症。值得注意的是，TNFα和TNFβ大约有30%的氨基酸具有同源性，并表现出相似的生物活性，这使得TNFα成为免疫系统应对感染和疾病的关键因素。了解 TNFα 的功能至关重要，因为 TNFα 不仅能促进机体的防御机制，还能在自身免疫性疾病和癌症等病理情况中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TNF alpha 抗体 (52B83) 参考文献:

  1. 系统性硬化症中 TNF-α 和 TNF-beta 基因的多态性。  |  Pandey, JP. and Takeuchi, F. 1999. Hum Immunol. 60: 1128-30. PMID: 10600011
  2. 肿瘤坏死因子-α（TNF-α）在中耳胆固醇瘤骨吸收中的作用。  |  Vitale, RF. and Ribeiro, Fde A. 2007. Braz J Otorhinolaryngol. 73: 117-21. PMID: 17505610
  3. 肿瘤坏死因子。对其多重作用分子机制的新认识。  |  Vilcek, J. and Lee, TH. 1991. J Biol Chem. 266: 7313-6. PMID: 1850405
  4. 采用贝叶斯方法比较肿瘤坏死因子-α（TNF-α）抑制剂与非TNF生物制剂在类风湿关节炎患者中对TNF-α抑制剂反应不足时的疗效。  |  Kim, HL., et al. 2014. Arch Pharm Res. 37: 662-70. PMID: 24469604
  5. 人类淋巴毒素和肿瘤坏死因子基因：结构, 同源性和染色体定位。  |  Nedwin, GE., et al. 1985. Nucleic Acids Res. 13: 6361-73. PMID: 2995927
  6. 肿瘤坏死因子α（TNF-α）通过靶向 Sirtuin1（SIRT1）增强 miR-155 介导的内皮细胞衰老。  |  Guo, Q., et al. 2019. Med Sci Monit. 25: 8820-8835. PMID: 31752013
  7. 使用网络药理学和分子对接技术鉴定类风湿性关节炎中的肿瘤坏死因子-α（TNF-α）抑制剂。  |  Bai, LL., et al. 2021. Front Pharmacol. 12: 690118. PMID: 34093213
  8. 人类肿瘤坏死因子。生产, 纯化和表征。  |  Aggarwal, BB., et al. 1985. J Biol Chem. 260: 2345-54. PMID: 3871770
  9. TNF 和淋巴毒素对 T 淋巴细胞的细胞毒作用。  |  Sarin, A., et al. 1995. J Immunol. 155: 3716-8. PMID: 7561073
  10. 淋巴毒素介导的肿瘤生长抑制：B 淋巴细胞参与抗肿瘤反应的证据。  |  Qin, Z. and Blankenstein, T. 1995. Cancer Res. 55: 4747-51. PMID: 7585497
  11. 缺乏淋巴毒素的小鼠外周淋巴器官发育异常。  |  De Togni, P., et al. 1994. Science. 264: 703-7. PMID: 8171322
  12. 肿瘤坏死因子的细胞毒活性由 p55 TNF 受体发出信号。  |  Tartaglia, LA., et al. 1993. Cell. 73: 213-6. PMID: 8386591

订购信息

产品名称产品编号规格价格数量收藏夹

TNF alpha 抗体 (52B83)

sc-52746
100 µg/ml
$316.00

TNF alpha (52B83): m-IgG Fc BP-HRP 套装

sc-538960
100 µg Ab; 10 µg BP
$354.00

TNF alpha (52B83): m-IgG1 BP-HRP 套装

sc-541177
100 µg Ab; 20 µg BP
$354.00

What is the dilution ration of TNF-a primary antibody for WB

Asked by: Biancocu
Thank you for your question . We recommend a dilution range of 1:200-1:1000. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2023-04-04

Does this antibody or do you have an antibody that recognize the N-terminal region of TNF?

Asked by: Harley
Thank you for your question . sc-52746 TNFα (52B83)is raised against the full length of the protein . The binding site has not been characterized. We do not offer a TNFa antibody for the N- terminal.
Answered by: BlakeJ
Date published: 2022-11-22

I would like to have a sample from two TNFa antibodies TNFa (C-4) sc133192 and TNFa-(52B83) sc-52746 to be able to decide which to order for my further experiments. Thank you in advance, T.B.

Asked by: Tzvete
Thank you for your question. Please contact Technical Service for information on how to receive free 10 ug samples of these monoclonal antibodies.
Answered by: Tech Service
Date published: 2025-03-21

Is this antibody knockout verified?

Asked by: UmNj
Thank you for your question. We have not performed any RNAi or CRISPR knockout or knockdown experiments to verify the specificity of this specific mouse monoclonal, TNFα Antibody (52B83): sc-52746. This particular antibody is very popular, and a well established clone that has been featured in over 50 publications, which are available on our website.
Answered by: Technical Support
Date published: 2017-03-16

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2016-12-16

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2016-12-16
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Rated 4 out of 5 by from Worked ok, although some non specific bindingThe antibody was received as a trial aliquot and stored at +4 °C upon arrival, as recommended. An aliquot was prepared by diluting 1:500 in PBS with 0.05% Tween-20 and 2% BSA. Application: Western blot on murine cell lysates. Experimental protocol: The antibody was used at a 1:500 dilution, as suggested in the datasheet. Standard blocking and washing steps were performed using PBS with 0.05% Tween-20 and 5% non-fat dry milk. Results: The antibody produced a clear band at the expected molecular weight. However, several non-specific bands were also detected across the membrane. This suggests that the antibody may have limited specificity under the tested conditions, or that further optimization of blocking, washing, or antibody dilution is required. Despite this, the sensitivity was sufficient to detect the target protein in 10 ug of total lysate. Additional comments: A high level of non-specific binding was observed, resulting in multiple background bands. We recommend optimizing the blocking and washing steps and testing lower antibody concentrations to reduce non-specific signals.
Date published: 2025-06-24
Rated 5 out of 5 by from Works well for WBWorks well for neonatal mouse lungs at the concentration of 30ug of protein. Ab dilution will be 1:200 for overnight at 4oC
Date published: 2024-12-13
Rated 4 out of 5 by from Buon funzionamento in WBHo provato questo anticorpo in WB (1:1000) e ha funzionato molto bene. Banda sull'altezza prevista di kDa.
Date published: 2021-10-22
Rated 5 out of 5 by from 很好之前买过，很不错的，实验很成功，以后会继续买的，会推荐同事购买，希望产品一如即往往的好，不错不错，非常开心
Date published: 2021-09-24
Rated 1 out of 5 by from BV2细胞的WB无法显示条带2周前买的抗体，我用无论是否使用LPS处理BV2细胞，WB的结果都是没有条带，我的dilution 1：200，loading protein是2.6ug，内参蛋白条带很清晰。除此之外，在santacruz购买的的TNF-α也有同样的问题，两者都没有任何条带。
Date published: 2021-09-07
Rated 1 out of 5 by from Nessun segnale in WBTestato su campioni di linea murina di macrofagi RAW264.7 stimolati con 10 o 100 ng/mL di LPS per 4 o 24 ore. L'anticorpo di un altro produttore ha funzionato.
Date published: 2021-04-23
Rated 5 out of 5 by from Good Western Blot resultsWe obtained a strong signal using this antibody in western blot. Band on predicted kDa height.
Date published: 2021-01-28
Rated 4 out of 5 by from Bene sia in immunofluoirescenza che in wbHo provato questo anticorpo in immunofluoresenza (1:100) su spinal cord di topo (fissato e criostatato), sia in wb (1:1000) e in entambi i casi ha funzionato molto bene
Date published: 2020-10-05
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TNF alpha 抗体 (52B83) is rated 4.2 out of 5 by 27.
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