TMB Substrate Buffer, 1X A single component, ready-to-use, working solution recommended for ELISA (microwell) procedures.

TMB Substrate Buffer, 1X

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应用; A single component, ready-to-use, working solution recommended for ELISA (microwell) procedures
* 参考分析证明 大量特定数据 (包括水 含量).
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TMB Substrate Buffer is a single component, ready-to-use, working solution recommended for ELISA (microwell) procedures. TMB (3, 3′, 5, 5′-tetramethylbenzidine) is a commonly used substrate for the enzyme horseradish peroxidase (HRP) and the combination is one of the most acknowledged ELISA detection systems. Develops a soluble blue reaction product that may be read at 655nm. For endpoint assays the reaction may be stopped with acid, forming a yellow reaction product that may be read at 450nm or 405nm. Not recommended for membrane applications.


参考文献

1. Reiter, Y., et al. 1995. Immunity. 2: 281-287. PMID: 7697545

用法 :
• This substrate is very light sensitive and must be protected from direct sunlight or any UV source.

• Bring to room temperature (approx. 25°C) before use. Note: Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended
that the antibodies or conjugates be diluted.

• Add 100 μl of TMB Substrate Buffer to each well and incubate for 5 –10 minutes at room temperature.

• A blue colored reaction product will develop, and negative control reactions remain clear non-colored solution in the wells.

• Add 100 μl of 1N H2SO4 to stop enzyme reaction after 10 minutes incubation. The blue color solution will turn to a yellow color.

•Blue reaction product, read at 655nm. Yellow, read at 450nm.
物理状态 :
Liquid
保存 :
Store at 4° C
仅供科研使用。不可用于诊断或治疗。

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分析证明书

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PMID: # 26608025  Kim, JI. et al. 2015. BMC Immunol. 16: 71.

PMID: # 23510534  Chen, JK. et al. 2013. ACS applied materials & interfaces. 5: 3348-55.

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