TLR4 CRISPR/Cas9 KO Plasmid (bovine): sc-437333
- Target species: bovine
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TLR4 CRISPR/Cas9 Knockout (KO) Plasmid (bovine) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TLR4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLR4 CRISPR/Cas9 KO Plasmid (bovine) | sc-437333 | 20 µg | $397.00 |
Overview
Toll-like receptor 4 (TLR4) is a pattern-recognition receptor that detects lipopolysaccharide and other pathogen- and damage-associated ligands to initiate innate immune signaling in bovine myeloid and epithelial cells. Ligand engagement drives MyD88- and TRIF-dependent pathways, activating NF-κB and IRF transcriptional programs that regulate proinflammatory cytokines, chemokines, and type I interferon responses. TLR4 signaling also intersects with MAPK cascades and inflammasome priming, shaping leukocyte recruitment and tissue-level inflammatory tone. Dysregulated TLR4 activity has been linked to bovine inflammatory and infectious disease phenotypes, including mastitis and endotoxin-driven systemic responses, making it relevant for studying host–pathogen interactions and immune homeostasis.
TLR4 CRISPR/Cas9 KO Plasmid (bovine) is a pool of plasmids designed for targeted disruption of the gene in bovine cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TLR4 protein expression.
This CRISPR knockout system enables efficient generation of -deficient cell models for investigation of TLR4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting exon(s) critical for TLR4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TLR4 CRISPR/Cas9 KO Plasmid (bovine) and TLR4 CRISPR/Cas9 KO Plasmid (bovine2) target distinct sites within the locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TLR4 HDR Plasmid (bovine) and TLR4 HDR Plasmid (bovine2) contain a puromycin resistance cassette and an RFP reporter flanked by homology arms to support homology-directed repair at defined target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.