Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TIGAR CRISPR Activation Plasmid (h): sc-401390-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TIGAR CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- TIGAR CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by TIGAR CRISPR Activation Plasmid (h) and TIGAR CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the TIGAR transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TIGAR Antibody (E-2): sc-166290
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TIGAR CRISPR Activation Plasmid (h) | sc-401390-ACT | 20 µg | $397.00 | |||
TIGAR CRISPR Activation Plasmid (h2) | sc-401390-ACT-2 | 20 µg | $397.00 |
TIGAR (TP53-induced glycolysis and apoptosis regulator) encodes a p53-responsive protein that functions as a fructose-2,6-bisphosphatase, lowering glycolytic flux while favoring pentose phosphate pathway activity to support NADPH production and redox homeostasis. By modulating intracellular reactive oxygen species and metabolic balance, TIGAR influences apoptosis, autophagy, and cell survival under oxidative or genotoxic stress. This metabolic checkpoint intersects with p53 signaling and broader stress-response networks that shape proliferation and damage tolerance. Dysregulated TIGAR expression has been associated with altered tumor metabolism and stress adaptation across multiple cancer contexts, making it a useful target for studying metabolic vulnerabilities and redox-regulated cell fate decisions.
TIGAR CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous TIGAR expression without altering the underlying DNA sequence.
TIGAR CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the TIGAR locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the TIGAR transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous TIGAR expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native TIGAR locus and enabling the study of TIGAR-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of TIGAR pathway restoration in tumor cells with silenced or reduced TIGAR expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.