Date published: 2025-9-6
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Tiam1 Antibody (E-7) - Western Blotting - Image 378682
Tiam1 抗体 (E-7): sc-393315. Jurkat (A), HEK293T (B), TK-1 (C), F9 (D), SUP-T1 (E) 和 ALL-SIL (F) 全细胞裂解液中 Tiam1 表达的 Western 印迹分析.
Tiam1 抗体 (E-7): sc-393315. Jurkat (A), HEK293T (B), TK-1 (C), F9 (D), SUP-T1 (E) 和 ALL-SIL (F) 全细胞裂解液中 Tiam1 表达的 Western 印迹分析.

Tiam1 抗体 (E-7): sc-393315

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说明书
  • Tiam1 抗体 E-7 是小鼠单克隆 IgG1 κ，Tiam1抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的Tiam1的N-terminus
  • Tiam1 抗体 (E-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Tiam1
  • 抗Tiam1抗体（E-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Tiam1 (E-7): sc-393315的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Tiam1 Antibody (E-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Tiam1 Antibody (E-7) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Tiam抗体，包括Tiam2

Tiam1抗体（E-7）是一种IgG1 κ小鼠单克隆Tiam1抗体（也被称为TIAM1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Tiam1蛋白。Tiam1抗体（E-7）既有非偶联的抗Tiam1抗体形式，也有多种偶联形式的抗Tiam1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。一个名为Tiam1的基因最初是通过原病毒标记结合体外选择侵袭性来鉴定为侵袭诱导基因。将截断的Tiam1 cDNA转染到非侵袭性细胞中，可以使这些细胞具有侵袭性。预测的Tiam1蛋白表现出Dbl和Pleckstrin同源结构域的特征，这些特征是GDP-GTP交换蛋白对于类似Rho蛋白（涉及细胞骨架组织）的特性。在成纤维细胞中，Tiam1诱导产生与组成性激活（V12）Rac1相似的表型，包括膜皱褶，但这种表型会被显性负性（N17）Rac1所抑制。此外，表达（V12）Rac1的T淋巴瘤细胞具有侵袭性，这支持了Tiam1-Rac信号通路可能参与肿瘤细胞侵袭和转移的观点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Tiam1 抗体 (E-7) 参考文献:

  1. Ras 相关 GTP 酶和细胞骨架  |  Hall, A. 1992. Mol Biol Cell. 3: 475-9. PMID: 1611153
  2. dbl 癌基因产物对 CDC42Hs 蛋白上鸟嘌呤核苷酸交换的催化作用。  |  Hart, MJ., et al. 1991. Nature. 354: 311-4. PMID: 1956381
  3. Tiam1与肝癌转移有关。  |  Huang, J., et al. 2013. Int J Cancer. 132: 90-100. PMID: 22573407
  4. 平衡的Tiam1-Rac1和RhoA驱动胰腺癌细胞的增殖和侵袭。  |  Guo, X., et al. 2013. Mol Cancer Res. 11: 230-9. PMID: 23322732
  5. Tiam1在乳腺导管浸润性癌中的过度表达的临床病理学意义。  |  Li, Z., et al. 2016. BMC Cancer. 16: 681. PMID: 27562113
  6. Tiam1介导的Rac1激活减少是导致小耳畸形软骨细胞迁移方向性持续性受损的原因。  |  Wu, Y., et al. 2024. J Cell Mol Med. 28: e18443. PMID: 38837873
  7. 小分子量 GTP 结合蛋白 rho 的 ADP 核糖基化抑制 PMA 诱导的 LFA-1 依赖性淋巴细胞聚集。  |  Tominaga, T., et al. 1993. J Cell Biol. 120: 1529-37. PMID: 7680658
  8. Rac 在 Tiam1 诱导的膜皱褶和侵袭中的作用  |  Michiels, F., et al. 1995. Nature. 375: 338-40. PMID: 7753201
  9. 一种新型癌基因 ost 编码一种鸟嘌呤核苷酸交换因子，它可能连接 Rho 和 Rac 信号通路。  |  Horii, Y., et al. 1994. EMBO J. 13: 4776-86. PMID: 7957046
  10. 鉴定入侵诱导基因Tiam-1，该基因编码的蛋白质与Rho-like蛋白的GDP-GTP交换器具有同源性。  |  Habets, GG., et al. 1994. Cell. 77: 537-49. PMID: 7999144
  11. 控制酵母芽点组装 GTPase Cdc42。催化 Cdc24 的鸟嘌呤核苷酸交换和刺激 Bem3 的 GTPase 活性。  |  Zheng, Y., et al. 1994. J Biol Chem. 269: 2369-72. PMID: 8300560
  12. rho p21 及其抑制性 GDP/GTP 交换蛋白（rho GDI）参与细胞运动。  |  Takaishi, K., et al. 1993. Mol Cell Biol. 13: 72-9. PMID: 8417362

订购信息

产品名称产品编号规格价格数量收藏夹

Tiam1 抗体 (E-7)

sc-393315
200 µg/ml
$316.00

Tiam1 (E-7): m-IgG Fc BP-HRP 套装

sc-530427
200 µg Ab; 10 µg BP
$354.00

Tiam1 (E-7): m-IgGκ BP-HRP 套装

sc-523901
200 µg Ab, 40 µg BP
$354.00

Tiam1 (E-7): m-IgG1 BP-HRP 套装

sc-544046
200 µg Ab; 20 µg BP
$354.00

Tiam1 抗体 (E-7) AC

sc-393315 AC
500 µg/ml, 25% agarose
$416.00

Tiam1 抗体 (E-7) HRP

sc-393315 HRP
200 µg/ml
$316.00

Tiam1 抗体 (E-7) FITC

sc-393315 FITC
200 µg/ml
$330.00

Tiam1 抗体 (E-7) PE

sc-393315 PE
200 µg/ml
$343.00

Tiam1 抗体 (E-7) Alexa Fluor® 488

sc-393315 AF488
200 µg/ml
$357.00

Tiam1 抗体 (E-7) Alexa Fluor® 546

sc-393315 AF546
200 µg/ml
$357.00

Tiam1 抗体 (E-7) Alexa Fluor® 594

sc-393315 AF594
200 µg/ml
$357.00

Tiam1 抗体 (E-7) Alexa Fluor® 647

sc-393315 AF647
200 µg/ml
$357.00

Tiam1 抗体 (E-7) Alexa Fluor® 680

sc-393315 AF680
200 µg/ml
$357.00

Tiam1 抗体 (E-7) Alexa Fluor® 790

sc-393315 AF790
200 µg/ml
$357.00

Can Tiam1 (E-7): sc-393315 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Tiam1 (E-7): sc-393315 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from does not work in fixed skinPurchased 1 month ago. Tried to probe for Tiam1 expression in paraformaldehyde fixed, OCT embedded mouse skin. Antibody did not work.
Date published: 2018-03-12
Rated 5 out of 5 by from Good for WBI bought sample antibody to test the expression of Tiam1 in HEK 293 cells after over-expressing a GPCR. I got very clean and sharp bands. I'm sure to purchase this antibody for my further experiments.
Date published: 2017-12-17
Rated 5 out of 5 by from Effect is very goodThe researchers confirmed thatTiam1 Antibody accurately detects human/rat with high sensitivity and specificity. It shows hight express and distinct.
Date published: 2017-10-18
Rated 5 out of 5 by from The product is good.The analysis results of Western blot were very strong and clear, Tiam1 Antibody suitable for a variety of cells.
Date published: 2017-10-18
Rated 5 out of 5 by from Tiam1 antibody (E-7) validated by siRNA knock downTiam1 was downregulated by siRNA in mouse primary microglia in order to validate the Tiam1 (E-7) sc-393315 antibody by Western Blot. In cells transfected with Tiam1 siRNA, the antigen band was hardly visible compared to control cells (scrambled siRNA transfected cells and untransfected cells). This result demonstrates the specificity of this antibody and makes it to a valuable research tool.
Date published: 2017-08-31
Rated 5 out of 5 by from It's a superb antibodyTiam1 Antibody are involved in some advantages, high specificity, sensitivity, convenience and quickness. It's your ideal choice to do drugtest.
Date published: 2017-02-13
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Tiam1 expression in HuT 78 and Ramos whole cell lysates.
Date published: 2017-02-06
Rated 5 out of 5 by from Produced positive Western blot data of Tiam1Produced positive Western blot data of Tiam1 expression in HuT 78 and Ramos whole cell lysates. -SCBT QC
Date published: 2014-03-15
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Tiam1 抗体 (E-7) is rated 4.5 out of 5 by 8.
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