Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
THOC1 CRISPR/Cas9 KO Plasmid (h): sc-403390
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- THOC1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the THOC1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: THOC1 Antibody (E-10): sc-514123
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
THOC1 CRISPR/Cas9 KO Plasmid (h) | sc-403390 | 20 µg | $397.00 |
Overview
THOC1 encodes a core subunit of the THO/TREX complex, which couples transcription to mRNA processing and nuclear export by coordinating co-transcriptional messenger ribonucleoprotein (mRNP) assembly. Through interactions with RNA polymerase II and export factors, THOC1 supports genome-wide transcript maturation, safeguards R-loop homeostasis, and helps maintain replication and transcriptional integrity. Disruption of THOC1-dependent RNA export can perturb cell-cycle progression and stress responses, linking this factor to pathways that influence genome stability. Altered THOC1 expression and TREX complex dysfunction have been observed in multiple cancer-related contexts and in studies of RNA metabolism disorders, making THOC1 a useful node for mechanistic investigations.
THOC1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the THOC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the THOC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the THOC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish THOC1 protein expression.
This CRISPR knockout system enables efficient generation of THOC1-deficient cell models for investigation of THOC1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting THOC1 exon(s) critical for THOC1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple THOC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by THOC1 CRISPR/Cas9 KO Plasmid (h) and THOC1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the THOC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by THOC1 HDR Plasmid (h) and THOC1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by THOC1 homology arms to support homology-directed repair at defined THOC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.