Date published: 2025-9-6

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TGF beta Receptor 3/TGFBR3 抗体 (A-4): sc-74511

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说明书
  • TGF beta Receptor 3/TGFBR3 抗体 A-4 是小鼠单克隆 IgG1 κ,TGF beta Receptor 3/TGFBR3抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫human来源TGFβ RIII近C-terminus的511-790氨基酸
  • TGF beta Receptor 3/TGFBR3 抗体 (A-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 TGFβ RIII
  • 抗TGF beta Receptor 3/TGFBR3抗体(A-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TGF beta Receptor 3/TGFBR3 (A-4): sc-74511免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 TGF beta Receptor 3/TGFBR3 抗体 (A-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与TGF beta Receptor 3/TGFBR3 抗体 (A-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

TGFβ RIII抗体(A-4)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的TGFβ RIII蛋白。TGFβ RIII (A-4) 抗体有非结合型和多种结合型可供选择,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。TGFβ RIII,也称为TGFBR3或β-葡聚糖,在转化生长因子β信号传导通路中起着至关重要的作用,该通路可调节细胞生长、分化及免疫反应。TGFβ RIII主要位于细胞表面,捕获并呈现TGFβ给信号传导受体,增强影响细胞过程的信号级联。TGFβ RIII是一种分泌型单通道I型膜蛋白,由850个氨基酸组成,具有ZP结构域,可促进与TGFβ配体的相互作用。TGFβ RIII在翻译后经历了显著的修饰,包括糖胺聚糖基团的附着,这对功能和稳定性至关重要。TGFβ RIII基因位于人类1p22.2染色体上,突出了物种间进化的保守性和重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TGF beta Receptor 3/TGFBR3 抗体 (A-4) 参考文献:

  1. 表达克隆 TGF-beta II 型受体--一种功能性跨膜丝氨酸/苏氨酸激酶。  |  Lin, HY., et al. 1992. Cell. 68: 775-85. PMID: 1310899
  2. TGF beta 通过异构蛋白激酶受体复合物发出信号。  |  Wrana, JL., et al. 1992. Cell. 71: 1003-14. PMID: 1333888
  3. 不同的转化生长因子-β(TGF-β)受体亚群是细胞对三种 TGF-beta 异构体反应的决定因素。  |  Cheifetz, S., et al. 1990. J Biol Chem. 265: 20533-8. PMID: 1700790
  4. TGF-β家族膜结合辅助受体β-葡聚糖和内皮素的结构生物学。  |  Kim, SK., et al. 2019. Exp Biol Med (Maywood). 244: 1547-1558. PMID: 31601110
  5. 鉴定转化生长因子β型基因家族的另一个成员。  |  ten Dijke, P., et al. 1988. Proc Natl Acad Sci U S A. 85: 4715-9. PMID: 3164476
  6. β-葡聚糖的糖胺聚糖修饰调节外切结构域脱落,从而微调卵巢癌中的TGF-β信号传导反应。  |  Choi, AS., et al. 2024. Cell Commun Signal. 22: 128. PMID: 38360757
  7. 人类转化生长因子-β互补 DNA 序列及在正常细胞和转化细胞中的表达。  |  Derynck, R., et al. Nature. 316: 701-5. PMID: 3861940
  8. TGF-β与β-葡聚糖和信号受体的结构揭示了β-葡聚糖增强受体复合物组装和信号传导的机制。  |  Wieteska, Ł., et al. 2024. bioRxiv.. PMID: 39091787
  9. TGFBR3在肺癌发展中的作用。  |  Deng, X., et al. 2024. Protein Pept Lett. 31: 491-503. PMID: 39092729
  10. 来自病毒转化细胞条件培养基的肉瘤生长因子由α型和β型转化生长因子组成。  |  Anzano, MA., et al. 1983. Proc Natl Acad Sci U S A. 80: 6264-8. PMID: 6604914
  11. 转化生长因子(TGF)-beta 2 与 I 型和 II 型 TGF-beta 受体的合作结合。  |  Rodriguez, C., et al. 1995. J Biol Chem. 270: 15919-22. PMID: 7608141
  12. Betaglycan 有多个与转化生长因子-β 1 结合的位点。  |  Kaname, S. and Ruoslahti, E. 1996. Biochem J. 315 (Pt 3): 815-20. PMID: 8645162

订购信息

产品名称产品编号规格价格数量收藏夹

TGF beta Receptor 3/TGFBR3 抗体 (A-4)

sc-74511
200 µg/ml
$316.00

TGF beta Receptor 3/TGFBR3 (A-4): m-IgG Fc BP-HRP 套装

sc-527033
200 µg Ab; 10 µg BP
$354.00

TGF beta Receptor 3/TGFBR3 (A-4): m-IgG1 BP-HRP 套装

sc-532406
200 µg Ab; 20 µg BP
$354.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) AC

sc-74511 AC
500 µg/ml, 25% agarose
$416.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) HRP

sc-74511 HRP
200 µg/ml
$316.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) FITC

sc-74511 FITC
200 µg/ml
$330.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) PE

sc-74511 PE
200 µg/ml
$343.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) Alexa Fluor® 488

sc-74511 AF488
200 µg/ml
$357.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) Alexa Fluor® 546

sc-74511 AF546
200 µg/ml
$357.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) Alexa Fluor® 594

sc-74511 AF594
200 µg/ml
$357.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) Alexa Fluor® 647

sc-74511 AF647
200 µg/ml
$357.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) Alexa Fluor® 680

sc-74511 AF680
200 µg/ml
$357.00

TGF beta Receptor 3/TGFBR3 抗体 (A-4) Alexa Fluor® 790

sc-74511 AF790
200 µg/ml
$357.00

Can TGFβ RIII (A-4): sc-74511 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TGFβ RIII (A-4): sc-74511 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Produces multiple bands in airway smooth muscleWestern using 16ug of total protein from human airway smooth muscle cells produced multiple bands (bands at 60, 150 and 250 kDa were the strongest with additional bands at 40 & 100 kDa).
Date published: 2017-07-31
Rated 5 out of 5 by from 性价比高文献中推荐了该抗体,自己用,果然不错,推荐他人购买!比其他公司的抗体,价格实惠,量也够用!相信该品牌!
Date published: 2017-06-07
Rated 5 out of 5 by from Excellent!This antibody is great in Western blot analysis of human recombinant TGFβ RIII under reducing conditions.
Date published: 2017-01-26
Rated 5 out of 5 by from Good WB analysisIt works well to detect TGFBR3 in total protein of nucleus pulposus cells, with the dilution ration 1:500.
Date published: 2016-11-23
Rated 5 out of 5 by from Nice membrane and cytoplasmic staining ofNice membrane and cytoplasmic staining of glomerular cells and cytoplasmic staining of cells in tubules of formalin fixed, paraffin-embedded human kidney tissue. - SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by from Good Western blot analysis of TGFb RIII expressionGood Western blot analysis of TGFb RIII expression in ECV304 whole cell lysate. - SCBT QC
Date published: 2015-01-04
Rated 4 out of 5 by from Satisfactory Western blot analysis of humanSatisfactory Western blot analysis of human recombinant TGFb RIII under reducing conditions. - SCBT QC
Date published: 2013-06-11
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TGF beta Receptor 3/TGFBR3 抗体 (A-4) is rated 4.6 out of 5 by 7.
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