Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
tetranectin CRISPR/Cas9 KO Plasmid (m): sc-423429
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- tetranectin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the tetranectin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: tetranectin Antibody (F-8): sc-376940
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
tetranectin CRISPR/Cas9 KO Plasmid (m) | sc-423429 | 20 µg | $397.00 |
Overview
Mouse Clec3b encodes tetranectin, a secreted C-type lectin–like protein found in extracellular fluids and matrix-associated compartments. Tetranectin binds plasminogen and has been linked to regulation of pericellular proteolysis, fibrinolysis, and extracellular matrix remodeling, processes that influence cell migration and tissue repair. Through its interactions with protease systems and matrix components, tetranectin can modulate inflammatory and stromal microenvironments and has been studied in the context of tumor invasion, wound healing, and fibrotic remodeling. Altered CLEC3B expression or protein abundance has been reported in multiple disease settings, supporting its use as a mechanistic node for investigating proteolytic balance and matrix dynamics.
tetranectin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Clec3b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Clec3b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Clec3b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish tetranectin protein expression.
This CRISPR knockout system enables efficient generation of Clec3b-deficient cell models for investigation of tetranectin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Clec3b exon(s) critical for tetranectin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Clec3b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by tetranectin CRISPR/Cas9 KO Plasmid (m) and tetranectin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Clec3b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by tetranectin HDR Plasmid (m) and tetranectin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Clec3b homology arms to support homology-directed repair at defined Clec3b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.