Date published: 2025-9-7

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TARDBP 抗体 (H-8): sc-376532

5.0(5)
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说明书
  • TARDBP抗体(H-8)是小鼠单克隆IgG1 κ, 在13篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-89位于human物种的TARDBP的N-terminus
  • 抗-TARDBP 抗体 (H-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 TARDBP; 也和以下物种反应,包括: 和 equine, canine and bovine
  • 抗TARDBP抗体(H-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-376532 X, 200 µg/0.1 ml)
  • 关于如何获取TARDBP (H-8): sc-376532免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成TARDBP 抗体 (H-8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

TARDBP抗体(H-8)是一种IgG1 κ小鼠单克隆TARDBP抗体,能够通过WB, IP, IF和ELISA检测小鼠、大鼠和人的TARDBP。TARDBP抗体(H-8)既可以作为非偶联的抗TARDBP抗体形式,也可以作为多种偶联形式的抗TARDBP抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。TARDBP(TAR DNA结合蛋白),也被称为TDP-43,是一种含有两个RRM(RNA识别基序)结构域的核蛋白。它在各种组织中普遍表达,在胎盘、肺、胰腺、脾脏和生殖道中的表达水平最高。TARDBP作为DNA结合蛋白发挥作用,特异性地结合到HIV的TAR DNA序列基序上。通过与TAR基序的这种关联,TARDBP充当转录抑制因子并抑制HIV-1的转录。TARDBP还可以作为剪接活性的负调节因子,已知参与CFTR(囊性纤维化跨膜受体)的剪接。此外,TARDBP是泛素阳性包涵体的主要成分,在许多神经退行性疾病中都很突出。这表明TARDBP可能在神经退行性疾病的发展中发挥作用。由于存在选择性剪接事件,TARDBP存在各种异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

TARDBP 抗体 (H-8)

sc-376532
200 µg/ml
$316.00

TARDBP 抗体 (H-8) X

sc-376532 X
200 µg/0.1 ml
$316.00

TARDBP 抗体 (H-8) AC

sc-376532 AC
500 µg/ml, 25% agarose
$416.00

TARDBP 抗体 (H-8) HRP

sc-376532 HRP
200 µg/ml
$316.00

TARDBP 抗体 (H-8) FITC

sc-376532 FITC
200 µg/ml
$330.00

TARDBP 抗体 (H-8) PE

sc-376532 PE
200 µg/ml
$343.00

TARDBP 抗体 (H-8) Alexa Fluor® 488

sc-376532 AF488
200 µg/ml
$357.00

TARDBP 抗体 (H-8) Alexa Fluor® 546

sc-376532 AF546
200 µg/ml
$357.00

TARDBP 抗体 (H-8) Alexa Fluor® 594

sc-376532 AF594
200 µg/ml
$357.00

TARDBP 抗体 (H-8) Alexa Fluor® 647

sc-376532 AF647
200 µg/ml
$357.00

TARDBP 抗体 (H-8) Alexa Fluor® 680

sc-376532 AF680
200 µg/ml
$357.00

TARDBP 抗体 (H-8) Alexa Fluor® 790

sc-376532 AF790
200 µg/ml
$357.00

Do you have more detailed info on the epitope recognized by either of your N-terminal TDP-43 monoclonal antibodies H8 or E10, i.e. more than their being raised against residues 1-89? Also, which works best on blots?

Asked by: StanDUP
Thank you for your question. We have not further epitope mapped the recognition sites for any of our TARDBP antibodies. The (E-10) and (H-8) clones are both raised against the same recombinant protein corresponding to amino acids 1-89 of human origin, but they do recognize different epitopes within this range. Both antibodies work equally well in Western blotting, however we would recommend our TARDBP (E-10): sc-376311 because it has been cited in a publication for Western blot. For a limited time we are offering a free 10 µg sample of the mouse monoclonal antibody, TARDBP (E-10): sc-376311. Please contact our Technical Service Department to request a free sample. Please call us toll-free (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-11

What is the recommended fixation method for immunofluorescence staining with TARDBP (H-8): sc-376532 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from very good signal in WBAntibody detects well TDP43 , dilution 1:500 in TBST 5% milk
Date published: 2021-06-23
Rated 5 out of 5 by from Excellent for western blottingThis mouse antibody works perfectly for western blotting, clean and specific!
Date published: 2017-12-03
Rated 5 out of 5 by from Tardbp antibody hiqh qualityI have tested this antibody on different amount of mouse brain lysate (from 5 to 20 micrograms of total proteins)and primary colture of cerebellar granule neurons. It is very good as shown in the jpg that i send you.
Date published: 2017-04-20
Rated 5 out of 5 by from Great immunofluorescence nuclear stainingGreat immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Satisfactory Western Blot data of TARDBPSatisfactory Western Blot data of TARDBP expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-10-06
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TARDBP 抗体 (H-8) is rated 5.0 out of 5 by 5.
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