Date published: 2025-9-7
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TACE/ADAM17 Antibody (B-6) - Western Blotting - Image 290845
TACE 抗体 (B-6): sc-390859. 在 NAMALWA (A), K-562 (B), MOLT-4 (C), BJAB (D) 和 CCRF-HSB-2 (E) 全细胞裂解液中检测 TACE 表达的 Western 印迹分析.
TACE 抗体 (B-6): sc-390859. 在 NAMALWA (A), K-562 (B), MOLT-4 (C), BJAB (D) 和 CCRF-HSB-2 (E) 全细胞裂解液中检测 TACE 表达的 Western 印迹分析.

TACE/ADAM17 抗体 (B-6): sc-390859

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说明书
  • TACE/ADAM17 抗体 B-6 是小鼠单克隆 IgG1 κ，TACE/ADAM17抗体, 在21篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的TACE的N-terminus
  • TACE/ADAM17 抗体 (B-6) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 TACE precursor
  • 抗TACE/ADAM17抗体（B-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取TACE/ADAM17 (B-6): sc-390859的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是TACE/ADAM17 Antibody (B-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与TACE/ADAM17 Antibody (B-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

TACE抗体（B-6）是一种IgG1 κ型小鼠单克隆TACE抗体（也称为ADAM17抗体），可通过WB、IP、IF和ELISA检测人源TACE蛋白。TACE抗体（B-6）既有非偶联抗TACE抗体形式，也有多种偶联形式的抗TACE抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肿瘤坏死因子β（TNFβ），也称为淋巴毒素，是一种多效性细胞因子。TNFα，也称为恶病质素，是一种与相同受体结合的细胞因子，产生一系列与TNFβ相似的效果。TNFβ和TNFα具有30%的氨基酸同源性，并具有相似的生物学活性。TNFβ由活化的淋巴细胞产生，包括CD4(+) T辅助细胞1型淋巴细胞、CD8(+)淋巴细胞和某些B淋巴母细胞系。TNFα由几种不同的细胞类型产生，包括淋巴细胞、中性粒细胞和巨噬细胞。TNFβ和TNFα可以调节许多免疫和炎症功能，同时具有抑制肿瘤生长的能力。TACE（代表TNFα转换酶）是一种金属蛋白酶，可裂解膜结合的TNFα前体，以释放可溶性的TNFα。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TACE/ADAM17 抗体 (B-6) 参考文献:

  1. 肿瘤坏死因子。对其多重作用分子机制的新认识。  |  Vilcek, J. and Lee, TH. 1991. J Biol Chem. 266: 7313-6. PMID: 1850405
  2. 人类淋巴毒素和肿瘤坏死因子基因：结构, 同源性和染色体定位。  |  Nedwin, GE., et al. 1985. Nucleic Acids Res. 13: 6361-73. PMID: 2995927
  3. 肝细胞癌患者经动脉化疗栓塞（TACE）联合索拉非尼与单独TACE的随机、多中心前瞻性试验：TACTICS试验。  |  Kudo, M., et al. 2020. Gut. 69: 1492-1501. PMID: 31801872
  4. TACE-HAIC联合靶向治疗和免疫治疗对比单独TACE治疗肝细胞癌伴门静脉肿瘤栓塞：倾向评分匹配研究。  |  Yuan, Y., et al. 2023. Int J Surg. 109: 1222-1230. PMID: 37026861
  5. 125 I 照射支架联合 TACE 与索拉非尼联合 TACE 治疗伴有门静脉肿瘤栓塞的肝细胞癌：多中心随机试验。  |  Lu, J., et al. 2023. Int J Surg. 109: 1188-1198. PMID: 37038986
  6. TACE难治性的分子机制：TACE手术改进方向。  |  Li, J., et al. 2024. Life Sci. 342: 122540. PMID: 38428568
  7. 人类肿瘤坏死因子。生产, 纯化和表征。  |  Aggarwal, BB., et al. 1985. J Biol Chem. 260: 2345-54. PMID: 3871770
  8. 复发中期肝细胞癌患者的生存率：索拉非尼联合TACE与单独TACE的随机临床试验。  |  Fan, W., et al. 2024. JAMA Oncol. 10: 1047-1054. PMID: 38900435
  9. 淋巴毒素介导的肿瘤生长抑制：B 淋巴细胞参与抗肿瘤反应的证据。  |  Qin, Z. and Blankenstein, T. 1995. Cancer Res. 55: 4747-51. PMID: 7585497
  10. 缺乏淋巴毒素的小鼠外周淋巴器官发育异常。  |  De Togni, P., et al. 1994. Science. 264: 703-7. PMID: 8171322
  11. 一种金属蛋白酶分解蛋白，能从细胞中释放肿瘤坏死因子-α。  |  Black, RA., et al. 1997. Nature. 385: 729-33. PMID: 9034190
  12. 克隆可处理肿瘤坏死因子-α前体的崩解金属蛋白酶。  |  Moss, ML., et al. 1997. Nature. 385: 733-6. PMID: 9034191

订购信息

产品名称产品编号规格价格数量收藏夹

TACE/ADAM17 抗体 (B-6)

sc-390859
200 µg/ml
$316.00

TACE/ADAM17 (B-6): m-IgG1 BP-HRP 套装

sc-551726
200 µg Ab; 20 µg BP
$354.00

TACE/ADAM17 抗体 (B-6) AC

sc-390859 AC
500 µg/ml, 25% agarose
$416.00

TACE/ADAM17 抗体 (B-6) HRP

sc-390859 HRP
200 µg/ml
$316.00

TACE/ADAM17 抗体 (B-6) FITC

sc-390859 FITC
200 µg/ml
$330.00

TACE/ADAM17 抗体 (B-6) PE

sc-390859 PE
200 µg/ml
$343.00

TACE/ADAM17 抗体 (B-6) Alexa Fluor® 488

sc-390859 AF488
200 µg/ml
$357.00

TACE/ADAM17 抗体 (B-6) Alexa Fluor® 546

sc-390859 AF546
200 µg/ml
$357.00

TACE/ADAM17 抗体 (B-6) Alexa Fluor® 594

sc-390859 AF594
200 µg/ml
$357.00

TACE/ADAM17 抗体 (B-6) Alexa Fluor® 647

sc-390859 AF647
200 µg/ml
$357.00

TACE/ADAM17 抗体 (B-6) Alexa Fluor® 680

sc-390859 AF680
200 µg/ml
$357.00

TACE/ADAM17 抗体 (B-6) Alexa Fluor® 790

sc-390859 AF790
200 µg/ml
$357.00

Can this antibody (sc-390859) be used in mouse samples ?

Asked by: Hui G
Thank you for your question. We recommend the antibody for human samples.
Answered by: BlakeJ
Date published: 2024-05-28

Does this antibody (sc390859) work in tissue obtained from mice? I am interested in brain detection and I have tried other antibodies that work well in rat but not in mouse (although recommended).

Asked by: J_RP
Thank you for your question. TACE/ADAM17 Antibody (B-6) is only recommended for detection of TACE precursor of human origin by WB, IP, IF and ELISA. Based on our tests with this antibody it has been found to not be suitable for murine samples.
Answered by: Tech Support Europe
Date published: 2023-02-28

Hi, I am interesting in this antibody, can this antibody be used to detect ADAM17 in mouse lung? Thanks very your help.

Asked by: sherryayfy
Thank you for your question. TACE Antibody (B-6) is only recommended for detection of TACE precursor of human origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2021-08-25

HI is this tace c-15 antidy ADAM17?

Asked by: JCcui
Thank you for your question. Yes, this antibody will recognize ADAM17, also designated TACE, TNF-alpha-converting enzyme. You can always confirm reactivity information for our antibodies by checking complete protein and gene information on the data sheet, or the website under “Gene Info”, below the “Ordering Information”.
Answered by: Technical Support
Date published: 2019-01-26

this mAb can be used for detection by FACS of ADAM17 in jukat?

Asked by: Youssef
Thank you for your question. We do have data using this antibody to detect TACE, also designated ADAM17, in Jurkat by Western blot. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-26

I am interested in your antibody: TACE (B-6): sc-390859. From the description it appears it is meant for TACE precursor, however, I am interested in detecting the active form. Some product reviews appear to indicate it may work. Any further info?

Asked by: AASoto
Thank you for the question. TACE (B-6): sc-390859 was raised against amino acids 1-300 mapping at the N-terminus of TACE of human origin. The exact binding epitope has not been mapped, yet. The Pro-peptide comprises aas 18 – 214 and the active Chain 215 – 824. Data seems to indicate the active protein form will be detected. http://www.uniprot.org/uniprot/P78536
Answered by: Technical Support Europe
Date published: 2018-01-22

Can TACE (B-6): sc-390859 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, TACE (B-6): sc-390859 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works well in WBI used this antibody at 1:500-1:1000 dilution in WB to detect endogenous or transfected TACE. Validated in TACE-KO cell lines. Results are good.
Date published: 2021-06-24
Rated 1 out of 5 by from Does not work for flow cytometrysc-390859 antibody does not work for FCM on HFFF cells
Date published: 2019-05-01
Rated 4 out of 5 by from what is for FACShello please i need to know if this mAb work for FACS on the jurkat
Date published: 2018-08-21
Rated 5 out of 5 by from Great antibody, clear bands in western blot.I received a sample of this antibody (10ug/50ul). I tested it on protein extracts (30ug) of a cell line of trophoblasts induced to differentiate to an endothelial-like phenotype. First antibody dilution: 1: 1000 (0.2 ug/ml) Dilution second antibody (anti mouse - HRP): 1: 5000 Detection: ECL Exposure time: 45 sec. Approx.
Date published: 2018-05-14
Rated 5 out of 5 by from Works great for prodomain!I tested the antibody using purified recombinant prodomain of ADAM17 expressed in E. coli. Works quite well and I can see the band at the expected height.
Date published: 2018-02-08
Rated 5 out of 5 by from Great resultsTACE antibodies have been widely used in the laboratory and I recommend using 1:300, which always sends a very strong signal.
Date published: 2017-11-02
Rated 5 out of 5 by from Sehr gute Ergebnisse im Western Blot - hochspezifiSaubere Doppelbande in der korrekten Höhe - keine unspezifischen Banden über die gesamte Blotlänge
Date published: 2017-08-16
Rated 5 out of 5 by from Works with Western blots, limited backgroundI tried this with Molt-4, Jurkat and Loucy cells and saw 2 bands around 80 kDa. No background. Very clean.
Date published: 2017-08-10
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TACE/ADAM17 抗体 (B-6) is rated 4.6 out of 5 by 15.
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