Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SYT Double Nickase Plasmid (h): sc-401575-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SYT Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SYT Double Nickase Plasmid (h) and SYT Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SS18. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SYT Antibody (D-3): sc-390615
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SYT Double Nickase Plasmid (h) | sc-401575-NIC | 20 µg | $410.00 | |||
SYT Double Nickase Plasmid (h2) | sc-401575-NIC-2 | 20 µg | $410.00 |
SS18 (SYT) encodes a core subunit of the SWI/SNF (BAF) ATP-dependent chromatin remodeling complex that regulates chromatin accessibility and RNA polymerase II–dependent transcription. Through coordinating enhancer–promoter communication and lineage-specific gene expression programs, SS18 helps control cell cycle progression, differentiation, and developmental patterning. Aberrant SS18 biology is strongly implicated in synovial sarcoma, where SS18 translocation-derived fusion proteins rewire BAF complex composition and transcriptional outputs. SS18-associated chromatin dysregulation also informs broader studies of epigenetic control, oncogenic transcriptional dependencies, and genome organization in human cells.
SYT Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SS18 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SS18. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SS18 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SS18-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.