Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Synaptogyrin-1 CRISPR/Cas9 KO Plasmid (m): sc-423238
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synaptogyrin-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Synaptogyrin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synaptogyrin-1 Antibody (A-6): sc-393456
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synaptogyrin-1 CRISPR/Cas9 KO Plasmid (m) | sc-423238 | 20 µg | $397.00 |
Overview
Syngr1 encodes synaptogyrin-1, a synaptic vesicle membrane protein enriched at presynaptic terminals that contributes to vesicle organization and efficient neurotransmitter release. It participates in core synaptic vesicle cycling processes, including vesicle trafficking, docking, and endocytosis, and functionally intersects with pathways regulating exo-endocytic coupling and presynaptic membrane dynamics. In mouse neurons, synaptogyrin-1 is used as a marker and mechanistic node for studying synaptic transmission and plasticity at excitatory and inhibitory synapses. Altered synaptic vesicle handling and presynaptic protein networks involving synaptogyrin-1 are relevant to research on neurodevelopmental and neurodegenerative disease mechanisms characterized by synaptic dysfunction.
Synaptogyrin-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Syngr1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Syngr1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Syngr1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Synaptogyrin-1 protein expression.
This CRISPR knockout system enables efficient generation of Syngr1-deficient cell models for investigation of Synaptogyrin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Syngr1 exon(s) critical for Synaptogyrin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Syngr1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Synaptogyrin-1 CRISPR/Cas9 KO Plasmid (m) and Synaptogyrin-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Syngr1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Synaptogyrin-1 HDR Plasmid (m) and Synaptogyrin-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Syngr1 homology arms to support homology-directed repair at defined Syngr1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.