Date published: 2025-9-8
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STRO-1 Antibody (STRO-1) - Flow Cytometry - Image 156463
STRO-1 抗体 (STRO-1) PE: sc-47733 PE. Glycophorin A 阴性人类骨髓细胞的 FCM 分析. 象限标记根据同型对照正常小鼠 IgM: sc-2870 设置. 由弗雷德-哈钦森癌症研究中心的 Beverly Torok-Storb 慷慨提供
STRO-1 Antibody (STRO-1) - Flow Cytometry - Image 156463
STRO-1 抗体 (STRO-1) PE: sc-47733 PE. Glycophorin A 阴性人类骨髓细胞的 FCM 分析. 象限标记根据同型对照正常小鼠 IgM: sc-2870 设置. 由弗雷德-哈钦森癌症研究中心的 Beverly Torok-Storb 慷慨提供
STRO-1 抗体 (STRO-1) PE: sc-47733 PE. Glycophorin A 阴性人类骨髓细胞的 FCM 分析. 象限标记根据同型对照正常小鼠 IgM: sc-2870 设置. 由弗雷德-哈钦森癌症研究中心的 Beverly Torok-Storb 慷慨提供

STRO-1 抗体 (STRO-1): sc-47733

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说明书
  • STRO-1 抗体 STRO-1 是小鼠单克隆 IgM κ，STRO-1抗体, 在59篇文献中引用，规格为200 µg/ml
  • 针对 human CD34+ 骨髓细胞
  • 推荐用于 human 来源的STRO-1 IF 和 FCM检测; 可能与 murine bone marrow-derived stromal progenitors产生交联反应
  • 可与HRP （sc-47733 HRP），200 µg/ml，用于WB、IHC(P)和ELISA；以及phycoerythrin（sc-47733 PE）或FITC（sc-47733 FITC），200 µg/ml，用于IF、IHC(P)和FCM
  • 还可偶联PerCP-Cy5.5 (sc-47733 PCPC5, 100 tests in 2 ml)，用于IF, IHC(P) 和 FCM
  • 关于如何获取STRO-1 (STRO-1): sc-47733的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成STRO-1 抗体 (STRO-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

STRO-1抗体（STRO-1）是一种IgM κ小鼠单克隆STRO-1抗体（也称为STRO-1抗体），可通过IF和FCM检测人类来源的STRO-1蛋白。STRO-1抗体（STRO-1）既可以是非偶联的抗STRO-1抗体形式，也可以是多种偶联形式的抗STRO-1抗体，包括HRP、PE、FITC。STRO-1是从免疫人CD34 +骨髓细胞的小鼠中分离出的单克隆IgM。STRO-1是一种有价值的试剂，可用于骨髓、牙髓和血液组织样本，通过识别这一血系独有的表面抗原来富集此血系的间充质（间质，MSCs）细胞的亚群。MSCs具有续期和分化为各种间质组织系的能力。这些MSCs的特征吸引了许多研究人员的注意，以治疗几种人类疾病的细胞疗法的背景。在骨髓细胞中，纤维细胞簇形成细胞（CFU-F）的频率在STRO-1 + / Glycophorin A-群体中比STRO-1 + / Glycophorin A +群体大约增加100倍。STRO-1 +富集的骨髓细胞亚群可分化为间质系，包括具有血管平滑肌表型的支持造血的基质细胞、脂肪细胞、成骨细胞和软骨细胞。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

STRO-1 抗体 (STRO-1) 参考文献:

  1. 利用原代体外培养和 poly (A) PCR 对 STRO-1 阳性的人骨髓基质细胞进行成骨细胞分化和 mRNA 分析。  |  Byers, RJ., et al. 1999. J Pathol. 187: 374-81. PMID: 10398094
  2. 人出生后骨髓基质干细胞的端粒酶表达可促进骨形成。  |  Shi, S., et al. 2002. Nat Biotechnol. 20: 587-91. PMID: 12042862
  3. 胎儿肝脏基质由上皮向间质转化的细胞组成。  |  Chagraoui, J., et al. 2003. Blood. 101: 2973-82. PMID: 12506029
  4. 脂肪组织和骨髓多系细胞上干细胞动员缔合作用分子的差异表达。  |  De Ugarte, DA., et al. 2003. Immunol Lett. 89: 267-70. PMID: 14556988
  5. 从人体骨小梁中提取的多潜能间充质祖细胞的特征。  |  Tuli, R., et al. 2003. Stem Cells. 21: 681-93. PMID: 14595128
  6. 体外扩增的 Stro-1- 或 Stro-1+ 人类间充质干细胞向 NOD/SCID 小鼠的归巢及其在支持人类 CD34 细胞移植中的作用。  |  Bensidhoum, M., et al. 2004. Blood. 103: 3313-9. PMID: 14715641
  7. 人类牙周韧带多能产后干细胞研究。  |  Seo, BM., et al. Lancet. 364: 149-55. PMID: 15246727
  8. 基质衍生物因子-1能促进人类骨髓基质干细胞的生长, 存活和发育。  |  Kortesidis, A., et al. 2005. Blood. 105: 3793-801. PMID: 15677562
  9. 用于硬组织工程的可接近人类成体干细胞源。  |  Laino, G., et al. 2006. J Cell Physiol. 206: 693-701. PMID: 16222704
  10. 骨肉瘤中的干样细胞：对肿瘤发生的影响。  |  Gibbs, CP., et al. 2005. Neoplasia. 7: 967-76. PMID: 16331882
  11. 用于治疗射血分数降低的慢性心力衰竭的间充质 STRO-1/STRO-3+ 前体细胞。  |  Sundin, A., et al. 2023. Future Cardiol. 19: 567-581. PMID: 37933628
  12. 人骨髓基质祖细胞的分离, 特征和在造血过程中的功能活性。  |  Simmons, PJ., et al. 1994. Prog Clin Biol Res. 389: 271-80. PMID: 7700911

订购信息

产品名称产品编号规格价格数量收藏夹

STRO-1 抗体 (STRO-1)

sc-47733
200 µg/ml
$316.00

STRO-1 抗体 (STRO-1) HRP

sc-47733 HRP
200 µg/ml
$316.00

STRO-1 抗体 (STRO-1) FITC

sc-47733 FITC
200 µg/ml
$330.00

STRO-1 抗体 (STRO-1) PE

sc-47733 PE
200 µg/ml
$343.00

STRO-1 抗体 (STRO-1) PerCP-Cy5.5

sc-47733 PCPC5
100 tests in 2 ml
$351.00

I have bought the sc-47733 PCPC5. What is the recommended initial concentration of this antibody for use in flow cytometry?

Asked by: Rafaalves
Thank you for your question. We recommend adding 1 µg of fluorochrome-conjugated antibodies per 1 million cells. You can find our standard protocol for flow cytometry under this link: https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: Technical Support Europe
Date published: 2020-10-14

I have bought the STRO-1sc-47733 PCPC5. What is the recommended initial concentration of this antibody for use in flow cytometry?

Asked by: Rafaalves
Thank you for your question. We recommend adding 1 µg of fluorochrome-conjugated antibodies per 1 million cells. You can find our standard protocol for flow cytometry under this link: https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: Technical Support Europe
Date published: 2020-10-14

if I use STRO-1 Antibody (STRO-1) sc-47733 (no flurecense conjugated) for flow cytometry, which isotype is suitable? Thank you.

Asked by: YYFF
Thank you for your question. Here is the non-conjugated normal mouse IgM isotype control: https://www.scbt.com/scbt/product/normal-mouse-igm?requestFrom=search
Answered by: Tech Service
Date published: 2019-06-10

I have bought the STRO-1 SC-47733 FITC antibody, my question is, is it possible to compare FCM of this antibody with IgM-PE: sc-2870? Because FITC and PE is totally differ.

Asked by: pratama
Thank you for your question. If you are using the FITC conjugated version of this primary antibody (sc-47733 FITC), then you would want to select the corresponding isotype control that is also conjugated to FITC. In this case, normal mouse IgM-FITC: sc-2859 (https://www.scbt.com/scbt/product/normal-mouse-igm-fitc). This product can be found in the list of "Support Products" for "FCM" on the primary antibody product page on our website (https://www.scbt.com/scbt/pdp/supportProducts.jsp?productId=47733&productType=FCM).
Answered by: Technical Support
Date published: 2017-02-06

Can this antibody be used to detect STRO-1 of mouse origin?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 7
Date published: 2017-01-11

What sequence is this antibody raised against?

Asked by: AbPolly
Thank you for your question. This antibody was raised against human CD34+ bone marrow cells, not a synthetic sequence.
Answered by: Technical Support 7
Date published: 2017-01-11
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Rated 5 out of 5 by from greatused dilution 1: 150 overnight for immunofluorescence
Date published: 2018-04-10
Rated 5 out of 5 by from Works very wellWorks very well for WB,we got high specific band at expected position ,,also the high sensitivity (1:800).
Date published: 2017-09-02
Rated 5 out of 5 by from good signalFor IF and applicable in human cells and 1:500 dilution,strong signal.
Date published: 2017-05-31
Rated 5 out of 5 by from Good IF stainingThis antibody works well and can be used for IF with Human breast cancer cell, I suggest use diluiton 1:400.
Date published: 2017-05-28
Rated 5 out of 5 by from Works well for IFGood IF data obtained in HeLa samples, high specificity.
Date published: 2017-05-23
Rated 5 out of 5 by from Good STRO-1 for flowThis antibody is good for STRO-1 detection in flow cytometry and IF. Well published for these applications and direct conjugates are available to bypass the need for a secondary antibody to save time and resources.
Date published: 2017-01-23
Rated 5 out of 5 by from Strong FCM !!Nice data for FCM studies; need result for additional applications
Date published: 2017-01-15
Rated 5 out of 5 by from Produced great FCM data for Glycophorin AProduced great FCM data for Glycophorin A negative human bone marrow cells. -SCBT QC
Date published: 2014-10-26
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STRO-1 抗体 (STRO-1) is rated 5.0 out of 5 by 8.
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