SRA Antibody (7H1G1): sc-56777

SRA Antibody (7H1G1) is a mouse monoclonal IgG1 antibody that detects steroid receptor RNA activator (SRA) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. SRA plays a crucial role in mediating the transactivation of steroid hormone receptors, functioning both as an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. SRA protein is characterized by six essential RNA motifs that are vital for coactivation capabilities, underscoring its importance in regulating gene expression in response to steroid hormones. SRA is widely expressed across various normal tissues, with particularly high levels found in the liver and skeletal muscle, while exhibiting lower expression in the brain. Notably, SRA levels are elevated in breast tumors compared to normal tissue, suggesting a potential role in cancer biology. SRA overexpression has been shown to enhance estrogen receptor alpha transcriptional activity, and when cells are transfected with antisense oligodeoxynucleotides targeting SRA, a dose-dependent reduction in estrogen receptor alpha expression occurs. Additionally, SMRT/HDAC1 associated repressor protein (SHARP) interacts with SRA, inhibiting SRA′s ability to potentiate steroid receptor transcription, further highlighting the complex regulatory mechanisms involving this protein.

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SRA Antibody (7H1G1) References:

1. Steroid receptor RNA activator stimulates proliferation as well as apoptosis in vivo.  |  Lanz, RB., et al. 2003. Mol Cell Biol. 23: 7163-76. PMID: 14517287
2. The steroid receptor RNA activator protein is expressed in breast tumor tissues.  |  Chooniedass-Kothari, S., et al. 2006. Int J Cancer. 118: 1054-9. PMID: 16152589
3. Steroid Receptor RNA Activator - A nuclear receptor coregulator with multiple partners: Insights and challenges.  |  Colley, SM. and Leedman, PJ. 2011. Biochimie. 93: 1966-72. PMID: 21807064
4. Steroid receptor RNA activator (SRA) modification by the human pseudouridine synthase 1 (hPus1p): RNA binding, activity, and atomic model.  |  Huet, T., et al. 2014. PLoS One. 9: e94610. PMID: 24722331
5. The steroid receptor RNA activator protein (SRAP) controls cancer cell migration/motility.  |  Yan, Y., et al. 2015. FEBS Lett. 589: 4010-8. PMID: 26581859
6. Silencing of LncRNA steroid receptor RNA activator attenuates polycystic ovary syndrome in mice.  |  Li, Y., et al. 2019. Biochimie. 157: 48-56. PMID: 30391287
7. Steroid receptor RNA activator gene footprint in the progression and drug resistance of colorectal cancer through oxidative phosphorylation pathway.  |  Mahdevar, M., et al. 2021. Life Sci. 285: 119950. PMID: 34520769
8. Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation.  |  Kochumon, S., et al. 2021. Cells. 10: PMID: 34685582
9. Ratios of peripheral blood mononuclear cells to lncRNA steroid receptor RNA activator as new indicators of metabolic syndrome.  |  Zhao, L., et al. 2022. Endokrynol Pol. 73: 81-86. PMID: 35119092
10. Expression of Steroid Receptor RNA Activator 1 (SRA1) in the Adipose Tissue Is Associated with TLRs and IRFs in Diabesity.  |  Kochumon, S., et al. 2022. Cells. 11: PMID: 36552771

The preferred SRA antibody is SRA Antibody (E-5): sc-393240.