Date published: 2025-9-6

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SPT16 抗体 (D-3): sc-377028

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说明书
  • SPT16 抗体 D-3 是小鼠单克隆 IgG2b κ,SPT16抗体, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的SPT16的N-terminus的氨基酸4-20之间
  • SPT16 抗体 (D-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SPT16; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗SPT16抗体(D-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SPT16 (D-3): sc-377028免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 SPT16 抗体 (D-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SPT16 抗体 (D-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

SPT16抗体(D-3)是一种IgG2b κ小鼠单克隆SPT16抗体(也称为SUPT16H抗体),能够通过WB、IP、IF、IHC(P)和ELISA技术检测小鼠、大鼠和人类来源的SPT16蛋白。SPT16抗体(D-3)既以非偶联的抗SPT16抗体形式提供,也以多种偶联形式的抗SPT16抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。蛋白质编码基因的表达需要特定的转录因子、RNA聚合酶和各种辅助因子的结合。这些辅助因子被区分为组蛋白乙酰转移酶或ATP依赖的染色质重塑酶,其中包括FACT(促进染色质转录),它们促进包装成染色质的DNA上的转录起始。FACT是染色质模板转录所需的染色质特异性延长因子,它特异性地与核小体和组蛋白H2A/H2B二聚体相互作用,以促进转录时的核小体解聚。FACT代表SPT16(酿酒酵母Spt16/Cdc68蛋白的同源物)和高迁移率组(HMG)-1样蛋白结构特异性识别蛋白-1(SSRP-1)之间的复合物。与其他(HMG)结构域包含蛋白相似,后者以能够弯曲目标DNA为特征,SSRP1和小鼠直系同源物T160与血清反应因子(SRF)发生物理相互作用,并作为正协同调节蛋白参与调节SRF依赖性基因表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SPT16 抗体 (D-3) 参考文献:

  1. 染色质特异性转录延伸因子 FACT 由人类 SPT16 和 SSRP1 蛋白组成。  |  Orphanides, G., et al. 1999. Nature. 400: 284-8. PMID: 10421373
  2. 人类 SSRP1 在基因转录中具有依赖 Spt16 和不依赖 Spt16 的作用。  |  Li, Y., et al. 2007. J Biol Chem. 282: 6936-45. PMID: 17209051
  3. 染色质是转录机制的重要组成部分。  |  Felsenfeld, G. 1992. Nature. 355: 219-24. PMID: 1731219
  4. 非编码转录物是 spt16 突变细胞核小体不稳定性的驱动力  |  Feng, J., et al. 2016. Mol Cell Biol. 36: 1856-67. PMID: 27141053
  5. 在程序性基因组重排中,Pgm 内切酶的功能需要帕拉米库组蛋白伴侣蛋白 Spt16-1。  |  de Vanssay, A., et al. 2020. PLoS Genet. 16: e1008949. PMID: 32702045
  6. FACT 组蛋白伴侣蛋白亚基 SPT16 的磷酸化影响拟南芥 RNA 聚合酶 II 转录物起始位点的染色质。  |  Michl-Holzinger, P., et al. 2022. Nucleic Acids Res. 50: 5014-5028. PMID: 35489065
  7. 组蛋白 H2B Arg95 残基能有效地转录因子 Spt16,介导信息素响应途径 Ste5 的表达。  |  Sulaiman, AA., et al. 2023. Sci Rep. 13: 10189. PMID: 37349401
  8. 酿酒酵母 DNA 聚合酶 alpha 催化亚基与 Cdc68/Spt16 和 Pob3(一种类似 HMG1 的蛋白质)相互作用。  |  Wittmeyer, J. and Formosa, T. 1997. Mol Cell Biol. 17: 4178-90. PMID: 9199353
  9. FACT,一种通过核糖体促进转录本延伸的因子。  |  Orphanides, G., et al. 1998. Cell. 92: 105-16. PMID: 9489704
  10. HMG结构域蛋白SSRP1/PREIIBF参与了人类胚胎β样球蛋白基因的激活。  |  Dyer, MA., et al. 1998. Mol Cell Biol. 18: 2617-28. PMID: 9566881
  11. 调节 RNA 聚合酶 II 转录延伸活性的因子  |  Shilatifard, A. 1998. FASEB J. 12: 1437-46. PMID: 9806752
  12. 体外染色质模板转录需要 RSF 和 FACT。  |  LeRoy, G., et al. 1998. Science. 282: 1900-4. PMID: 9836642

订购信息

产品名称产品编号规格价格数量收藏夹

SPT16 抗体 (D-3)

sc-377028
200 µg/ml
$316.00

SPT16 (D-3): m-IgGκ BP-HRP 套装

sc-523158
200 µg Ab, 40 µg BP
$354.00

SPT16 (D-3): m-IgG2b BP-HRP 套装

sc-549091
200 µg Ab; 10 µg BP
$354.00

SPT16 抗体 (D-3) AC

sc-377028 AC
500 µg/ml, 25% agarose
$416.00

SPT16 抗体 (D-3) HRP

sc-377028 HRP
200 µg/ml
$316.00

SPT16 抗体 (D-3) FITC

sc-377028 FITC
200 µg/ml
$330.00

SPT16 抗体 (D-3) PE

sc-377028 PE
200 µg/ml
$343.00

SPT16 抗体 (D-3) Alexa Fluor® 488

sc-377028 AF488
200 µg/ml
$357.00

SPT16 抗体 (D-3) Alexa Fluor® 546

sc-377028 AF546
200 µg/ml
$357.00

SPT16 抗体 (D-3) Alexa Fluor® 594

sc-377028 AF594
200 µg/ml
$357.00

SPT16 抗体 (D-3) Alexa Fluor® 647

sc-377028 AF647
200 µg/ml
$357.00

SPT16 抗体 (D-3) Alexa Fluor® 680

sc-377028 AF680
200 µg/ml
$357.00

SPT16 抗体 (D-3) Alexa Fluor® 790

sc-377028 AF790
200 µg/ml
$357.00

SPT16 (D-3) 中和勝肽

sc-377028 P
100 µg/0.5 ml
$68.00

Hi, I was wondering if I can get a complimentary sample of this Antibody to check if that works for our experiments? https://www.scbt.com/scbt/product/spt16-antibody-d-3

Asked by: Poonam
Thank you for your question. Yes, for a limited time we are offering a free 10 µg sample of this antibody, SPT16 (D-3): sc-377028 that you can use to test before you place an order. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-08-23

What application is the blocking peptide sc-377028 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Review of sc-377028 (anti-SPT16)This antibody works fine to detect SPT16 but with detectable smear with main band. It's unclear whether this is due to post-translational modification or if it is specific to this particular cell line. Slower moving band is detectable out of the predicted size of SPT16.
Date published: 2017-03-28
Rated 5 out of 5 by from positive expression shown with western blotWe used this antibody for SPT16 expression detection with western blot. We got a positive band at the correct molecular weight for HeLa cells
Date published: 2017-02-02
Rated 4 out of 5 by from Good antibodyTested in HeLa whole cell lysates: Nice specific band, some unspecific bands
Date published: 2016-12-05
Rated 5 out of 5 by from Strong bands in Western blot analysis ofStrong bands in Western blot analysis of SPT16 expression in K-562 and HeLa nuclear extracts. -SCBT QC
Date published: 2015-05-21
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic, membrane and nuclear staining in formalin fixed, paraffin-embedded human lower stomach tissue. -SCBT QC
Date published: 2015-01-13
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SPT16 抗体 (D-3) is rated 4.4 out of 5 by 5.
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