SPT16 Antibody (D-3): sc-377028

SPT16 Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects SPT16 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-SPT16 antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPT16 plays a crucial role in transcription by functioning as a part of the FACT complex, which is essential for RNA polymerase II elongation during transcription of chromatin templates. This complex interacts with nucleosomes and facilitates histone disassembly, allowing transcription machinery to access DNA more effectively. SPT16 (D-3) antibody helps researchers study how SPT16 modulates gene expression by ensuring efficient transcription through tightly packed chromatin. SPT16 also interacts with structure-specific recognition protein-1 (SSRP-1), forming a complex that enhances transcriptional activity of serum response factors, influencing various cellular processes. Through interactions with other proteins and chromatin remodeling functions, SPT16 significantly impacts gene regulation and cellular function, making SPT16 (D-3) monoclonal antibody valuable for studying transcriptional mechanisms and chromatin dynamics.

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SPT16 Antibody (D-3) References:

1. The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins.  |  Orphanides, G., et al. 1999. Nature. 400: 284-8. PMID: 10421373
2. Human SSRP1 has Spt16-dependent and -independent roles in gene transcription.  |  Li, Y., et al. 2007. J Biol Chem. 282: 6936-45. PMID: 17209051
3. Chromatin as an essential part of the transcriptional mechanism.  |  Felsenfeld, G. 1992. Nature. 355: 219-24. PMID: 1731219
4. Noncoding Transcription Is a Driving Force for Nucleosome Instability in spt16 Mutant Cells.  |  Feng, J., et al. 2016. Mol Cell Biol. 36: 1856-67. PMID: 27141053
5. The Paramecium histone chaperone Spt16-1 is required for Pgm endonuclease function in programmed genome rearrangements.  |  de Vanssay, A., et al. 2020. PLoS Genet. 16: e1008949. PMID: 32702045
6. Phosphorylation of the FACT histone chaperone subunit SPT16 affects chromatin at RNA polymerase II transcriptional start sites in Arabidopsis.  |  Michl-Holzinger, P., et al. 2022. Nucleic Acids Res. 50: 5014-5028. PMID: 35489065
7. The histone H2B Arg95 residue efficiently recruits the transcription factor Spt16 to mediate Ste5 expression of the pheromone response pathway.  |  Sulaiman, AA., et al. 2023. Sci Rep. 13: 10189. PMID: 37349401
8. The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein.  |  Wittmeyer, J. and Formosa, T. 1997. Mol Cell Biol. 17: 4178-90. PMID: 9199353
9. FACT, a factor that facilitates transcript elongation through nucleosomes.  |  Orphanides, G., et al. 1998. Cell. 92: 105-16. PMID: 9489704
10. The HMG domain protein SSRP1/PREIIBF is involved in activation of the human embryonic beta-like globin gene.  |  Dyer, MA., et al. 1998. Mol Cell Biol. 18: 2617-28. PMID: 9566881
11. Factors regulating the transcriptional elongation activity of RNA polymerase II.  |  Shilatifard, A. 1998. FASEB J. 12: 1437-46. PMID: 9806752
12. Requirement of RSF and FACT for transcription of chromatin templates in vitro.  |  LeRoy, G., et al. 1998. Science. 282: 1900-4. PMID: 9836642

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SPT16 Antibody (D-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SPT16 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SPT16 Antibody (D-3) Specifications

Alternate Product Name: SPT16

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 4-20

Epitope Target: SPT16

Epitope Species: human

Product Reactivity/Detection: SPT16

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SPT16 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin