Spo11 Double Nickase Plasmid (h): sc-404432-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Spo11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Spo11 Double Nickase Plasmid (h) and Spo11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SPO11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Spo11 Antibody (C-4): sc-377161
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Spo11 Double Nickase Plasmid (h) | sc-404432-NIC | 20 µg | $410.00 |
Human SPO11 encodes Spo11, a meiosis-specific topoisomerase-like endonuclease that catalyzes programmed DNA double-strand break formation to initiate homologous recombination. Spo11-driven breaks enable synapsis and crossover formation through coordinated engagement of the DNA damage response and recombinational repair pathways, including ATM/ATR signaling and strand-exchange machinery. Perturbation of SPO11 function disrupts meiotic progression, leading to chromosomal mis-segregation and infertility phenotypes, and it is broadly relevant to studies of genome stability and recombination control.
Spo11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SPO11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SPO11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SPO11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SPO11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.