Sox9 Double Nickase Plasmid (h): sc-400143-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sox9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sox9 Double Nickase Plasmid (h) and Sox9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SOX9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sox9 Antibody (E-9): sc-166505
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sox9 Double Nickase Plasmid (h) | sc-400143-NIC | 20 µg | $410.00 | |||
Sox9 Double Nickase Plasmid (h2) | sc-400143-NIC-2 | 20 µg | $410.00 |
SOX9 encodes the transcription factor Sox9, a high-mobility group (HMG) DNA-binding protein that regulates lineage specification and differentiation programs across multiple tissues. In development and adult homeostasis, Sox9 integrates signaling inputs such as TGF-β/BMP, WNT/β-catenin, Hedgehog, and Notch to control gene networks involved in chondrogenesis, stem/progenitor maintenance, and epithelial cell fate decisions. SOX9 activity influences extracellular matrix production and cell cycle dynamics through direct transcriptional control of target loci and cooperation with partner transcription factors. Dysregulated SOX9 expression or function has been associated with congenital skeletal disorders and contributes to pathogenic states involving aberrant differentiation and oncogenic transcriptional programs, making it a widely used node for mechanistic studies of development and disease biology.
Sox9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SOX9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SOX9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SOX9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SOX9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.