Date published: 2025-9-6

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Sox-18 抗体 (D-8): sc-166025

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说明书
  • Sox-18 抗体 D-8 是小鼠单克隆 IgG1 κ,Sox-18抗体, 在20篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列161-300位于human物种的Sox-18的C-terminus附近
  • Sox-18 抗体 (D-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Sox-18
  • 抗Sox-18抗体(D-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-166025 X, 200 µg/0.1 ml)
  • 关于如何获取Sox-18 (D-8): sc-166025免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 Sox-18 抗体 (D-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Sox-18 抗体 (D-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Sox-18抗体(D-8)是一种IgG1 κ小鼠单克隆Sox-18抗体(也被称作SOX18抗体或SRY相关HMG盒转录因子18抗体),它通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的Sox-18蛋白。Sox-18抗体(D-8)可以作为非偶联的抗Sox-18抗体形式提供,也可以作为多种偶联形式的抗Sox-18抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Sox基因是一个与哺乳动物性别决定基因SRY相关的基因家族。这些基因同样包含编码HMG盒结构域的序列,该结构域负责序列特异性的DNA结合活性。Sox基因编码假定的转录调节因子,这些因子在发育过程中决定细胞命运和控制各种发育过程中发挥作用。高度复杂的Sox基因群至少聚集了40个不同的位点,这些位点在各种动物谱系中迅速分化。目前,已经鉴定出30个Sox基因。这个家族的成员在进化过程中被证明是保守的,并在动物发育过程中发挥关键作用。其中一些与人类疾病有关,包括性反转。Sox-18是一种含有384个氨基酸的核蛋白,含有一个HMG盒DNA结合结构域,属于Sox家族的转录调节因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Sox-18 抗体 (D-8) 参考文献:

  1. 人类 SOX18 同源物的 cDNA 克隆, 组织表达和染色体图谱。  |  Azuma, T., et al. 2000. J Hum Genet. 45: 192-5. PMID: 10807548
  2. SOX18 在内皮细胞中与 MEF2C 直接相互作用。  |  Hosking, BM., et al. 2001. Biochem Biophys Res Commun. 287: 493-500. PMID: 11554755
  3. 匹配 SOX:转录调节因子 SOX 家族的伙伴蛋白和辅助因子。  |  Wilson, M. and Koopman, P. 2002. Curr Opin Genet Dev. 12: 441-6. PMID: 12100890
  4. 20对sox:小鼠和人类sox转录因子基因家族的范围, 同源性和命名。  |  Schepers, GE., et al. 2002. Dev Cell. 3: 167-70. PMID: 12194848
  5. 转录因子基因 SOX18 的突变是隐性和显性下垂-淋巴水肿-三角肌症的基础。  |  Irrthum, A., et al. 2003. Am J Hum Genet. 72: 1470-8. PMID: 12740761
  6. 转录因子 SOX18 在人类冠状动脉粥样硬化病变中表达,并调节 DNA 合成和血管细胞生长。  |  García-Ramírez, M., et al. 2005. Arterioscler Thromb Vasc Biol. 25: 2398-403. PMID: 16179596
  7. SOX18 转录因子功能紊乱对肿瘤生长, 血管形成和内皮发育的影响  |  Young, N., et al. 2006. J Natl Cancer Inst. 98: 1060-7. PMID: 16882943
  8. SOX-18 控制着内皮特异性 claudin-5 基因的表达和屏障功能。  |  Fontijn, RD., et al. 2008. Am J Physiol Heart Circ Physiol. 294: H891-900. PMID: 18065521
  9. 淋巴管生成因子SOX 18:胃肿瘤分期的重要指标。  |  Eom, BW., et al. 2012. Int J Cancer. 131: 41-8. PMID: 21796627
  10. 17β-雌二醇和类黄酮对新发现的雌激素反应基因在大鼠神经节核衍生细胞系中表达的调控作用。  |  Amer, DA., et al. 2012. J Cell Physiol. 227: 3434-45. PMID: 22213181
  11. nestin 表达在血管生成和乳腺癌进展中的作用  |  Nowak, A., et al. 2018. Int J Oncol. 52: 527-535. PMID: 29345290
  12. 编码一种新的 HMG-box 转录因子的 Sox-18 的序列和表达。  |  Dunn, TL., et al. 1995. Gene. 161: 223-5. PMID: 7665083

订购信息

产品名称产品编号规格价格数量收藏夹

Sox-18 抗体 (D-8)

sc-166025
200 µg/ml
$316.00

Sox-18 (D-8): m-IgG1 BP-HRP 套装

sc-531673
200 µg Ab; 20 µg BP
$354.00

Sox-18 抗体 (D-8) X

sc-166025 X
200 µg/0.1 ml
$316.00

Sox-18 抗体 (D-8) AC

sc-166025 AC
500 µg/ml, 25% agarose
$416.00

Sox-18 抗体 (D-8) HRP

sc-166025 HRP
200 µg/ml
$316.00

Sox-18 抗体 (D-8) FITC

sc-166025 FITC
200 µg/ml
$330.00

Sox-18 抗体 (D-8) PE

sc-166025 PE
200 µg/ml
$343.00

Sox-18 抗体 (D-8) Alexa Fluor® 488

sc-166025 AF488
200 µg/ml
$357.00

Sox-18 抗体 (D-8) Alexa Fluor® 546

sc-166025 AF546
200 µg/ml
$357.00

Sox-18 抗体 (D-8) Alexa Fluor® 594

sc-166025 AF594
200 µg/ml
$357.00

Sox-18 抗体 (D-8) Alexa Fluor® 647

sc-166025 AF647
200 µg/ml
$357.00

Sox-18 抗体 (D-8) Alexa Fluor® 680

sc-166025 AF680
200 µg/ml
$357.00

Sox-18 抗体 (D-8) Alexa Fluor® 790

sc-166025 AF790
200 µg/ml
$357.00

Is it no difference with sc-166025 HRP and sc-166025 for WB?

Asked by: Student1234
Thank you for your question. Both the unconjugated and the HRP-conjugated versions of this antibody recognize the same epitope. HRP-conjugated antibodies do not require a secondary antibody and as such they generally produce less unspecific background and allow for a more efficient experimental setup.
Answered by: Tech Support Europe
Date published: 2020-12-28

Hi there! Is it possible to access particular batches of this antibody? Thanks!

Asked by: FFontaine
Thank you for your question. We typically offer only one batch/lot of an antibody at a time. Once that lot of a product is out of stock, a new one will be generated, and we are no longer able to provide a previous lot.
Answered by: Tech Service
Date published: 2020-01-02

When should the TransCruz reagent sc-166025 X be used instead of the standard form sc-166025?

Asked by: AbPolly
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from FACSWe detected a bit expression in Jurkat cells by intracellular staining.
Date published: 2024-05-30
Rated 5 out of 5 by from Great resultsSox-18 Antibody (D-8) produced nice clear band in various whole cell lysate samples with no background. This antibody also worked well in IHCP, producing robust staining
Date published: 2017-01-30
Rated 5 out of 5 by from Nice staining in paraffinNice staining in paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-01-06
Rated 3 out of 5 by from Good antibodyGood antibody, works in human transfected lysates.
Date published: 2014-04-08
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Sox-18 抗体 (D-8) is rated 4.0 out of 5 by 4.
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