Sodium Pyruvate Solution 100 mM (100X), liquid, sodium pyruvate is utilized as a component in culture broth and media. It is utilized by cells as an intermediate compound in the metabolism of carbohydrates, proteins, and fats. It is involved with amino acid metabolism and initiates the Kreb′s cycle. In thiamine deficiency, its oxidation is retarded and it accumulates in the tissues, especially in nervous structures. A protcol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. The 100 mM solution should be diluted 1:100 for most cell culture applications.
1. Brielmeier, M., et al. 1998. Nucleic Acids Res. 26: 2082-2085. PMID: 9547263
2. Geshi, M., et al. 2000. Biol. Reprod. 63: 1730-1734. PMID: 11090443
3. Jiang, X.,and Doyle, M.P. 2000. J. Clin. Microbiol. 38: 1984-1987. PMID: 10790135
4. Kuo, T.M., et al. 2002. Curr. Microbiol. 45: 265-271. PMID: 12192524