Date published: 2025-9-7

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SNX17 抗体 (H-10): sc-166957

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说明书
  • SNX17 抗体 H-10 是小鼠单克隆 IgG1 κ,SNX17抗体, 在9篇文献中引用,规格为200 µg/ml
  • 针对氨基酸270-470的抗原,在SNX17的C-terminus处映射,human来源
  • SNX17 抗体 (H-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 SNX17
  • 抗SNX17抗体(H-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SNX17 (H-10): sc-166957免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是SNX17 Antibody (H-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与SNX17 Antibody (H-10) 打包提供(请参阅下面的订购信息)。

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関連項目

SNX17抗体(H-10)是一种IgG1 κ小鼠单克隆SNX17抗体(也被称为SNX17抗体),可以通过WB、IP、IF和ELISA等方法检测来自小鼠、大鼠和人类的SNX17蛋白。SNX17抗体(H-10)既有非偶联的抗SNX17抗体形式,也有多种偶联形式的抗SNX17抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。分拣连接蛋白(SNX)是一个大型的亲水性蛋白质家族,它们与多种受体类型相互作用,参与细胞内转运,并含有一个特征性的 phox 同源(PX)结构域。SNX17普遍表达,含有一个与SNX1的PX结构域具有28%序列同一性的PX结构域,以及一个B41(FERM)结构域。SNX17基因位于2号染色体上,是细胞分拣机制的一部分,通过促进LRP(脂蛋白受体相关蛋白)的循环来调节其细胞表面水平。虽然SNX17的PX结构域与磷脂酰肌醇-3-磷酸相互作用以实现膜关联,但FERM结构域和羧基末端区域有助于LRP的结合。研究表明,SNX17定位于早期内体的限制膜和循环小管中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SNX17 抗体 (H-10) 参考文献:

  1. 分拣 nexin 家族的一个新成员与 P-selectin 的 C 端相互作用。  |  Florian, V., et al. 2001. Biochem Biophys Res Commun. 281: 1045-50. PMID: 11237770
  2. PX-domain蛋白SNX17与低密度脂蛋白受体家族成员相互作用,并调节低密度脂蛋白受体的内吞作用。  |  Stockinger, W., et al. 2002. EMBO J. 21: 4259-67. PMID: 12169628
  3. 低密度脂蛋白受体胞内结构域中的分拣图案与分拣 nexin-17 的新型结构域相互作用。  |  Burden, JJ., et al. 2004. J Biol Chem. 279: 16237-45. PMID: 14739284
  4. 分拣 nexin 17 可加速 P-选择素的内化并延缓其降解。  |  Williams, R., et al. 2004. Mol Biol Cell. 15: 3095-105. PMID: 15121882
  5. 分拣 nexin 17 结构域的功能和 P-选择素迁移的识别基调。  |  Knauth, P., et al. 2005. J Mol Biol. 347: 813-25. PMID: 15769472
  6. 分拣 nexin 17 可促进 LRP 在早期内质体中的再循环。  |  van Kerkhof, P., et al. 2005. EMBO J. 24: 2851-61. PMID: 16052210
  7. 预测人类不明基因的编码序列。II.通过分析来自人类细胞系 KG-1 的 cDNA 克隆,推导出 40 个新基因(KIAA0041-KIAA0080)的编码序列。  |  Nomura, N., et al. 1994. DNA Res. 1: 223-9. PMID: 7584044

订购信息

产品名称产品编号规格价格数量收藏夹

SNX17 抗体 (H-10)

sc-166957
200 µg/ml
$316.00

SNX17 (H-10): m-IgG Fc BP-HRP 套装

sc-529093
200 µg Ab; 10 µg BP
$354.00

SNX17 (H-10): m-IgGκ BP-HRP 套装

sc-521809
200 µg Ab, 40 µg BP
$354.00

SNX17 (H-10): m-IgG1 BP-HRP 套装

sc-543313
200 µg Ab; 20 µg BP
$354.00

SNX17 抗体 (H-10) AC

sc-166957 AC
500 µg/ml, 25% agarose
$416.00

SNX17 抗体 (H-10) HRP

sc-166957 HRP
200 µg/ml
$316.00

SNX17 抗体 (H-10) FITC

sc-166957 FITC
200 µg/ml
$330.00

SNX17 抗体 (H-10) PE

sc-166957 PE
200 µg/ml
$343.00

SNX17 抗体 (H-10) Alexa Fluor® 488

sc-166957 AF488
200 µg/ml
$357.00

SNX17 抗体 (H-10) Alexa Fluor® 546

sc-166957 AF546
200 µg/ml
$357.00

SNX17 抗体 (H-10) Alexa Fluor® 594

sc-166957 AF594
200 µg/ml
$357.00

SNX17 抗体 (H-10) Alexa Fluor® 647

sc-166957 AF647
200 µg/ml
$357.00

SNX17 抗体 (H-10) Alexa Fluor® 680

sc-166957 AF680
200 µg/ml
$357.00

SNX17 抗体 (H-10) Alexa Fluor® 790

sc-166957 AF790
200 µg/ml
$357.00

What is the recommended starting dilution for Western blot for sc-166957: SNX17 (H-10) monoclonal antibody?

Asked by: Cweed
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22

En un WB usando BMDC se me revelan dos línas de SNX17, una de 55 K y otra banda menos marcada de unos 60 K. Además, trabajo con dos líneas de SNX17 KD y también observo estas dos líneas, ambas se atenúan con el silenciamiento. ¿A qué puede deberse?Thanks

Asked by: Asturiana
Gracias por su pregunta. Hemos probado nuestros anticuerpos SNX17 en una variedad de diferentes líneas celulares y no hemos observado un patrón de bandas similar en Western blot. Recomendamos probar las mismas muestras usando un anticuerpo SNX17 diferente con un epítopo distinto para ver si observa el mismo resultado. Actualmente ofrecemos otros dos anticuerpos monoclonales de ratón a SNX17, sc-166597: SNX17 (E-12), que tiene un epítopo C-terminal, y sc-166585: SNX17 (D-10), que tiene un epítopo N-terminal. También recomendamos consultar la literatura primaria para ver si otros investigadores han observado un resultado similar con el antígeno SNX17. Thank you for your question. We have tested our SNX17 antibodies in a variety of different cell lines and have not observed a similar banding pattern in Western blot. We would recommend testing the same samples using a different SNX17 antibody with a distinct epitope to see if you observe the same result. We currently offer two other mouse monoclonal antibodies to SNX17, sc-166597: SNX17 (E-12), which has a C-terminal epitope, and sc-166585: SNX17 (D-10), which has an N-terminal epitope. We also recommend consulting the primary literature to see if any other researchers have observed a similar result with the SNX17 antigen.
Answered by: Tech Service 3
Date published: 2016-12-21
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Rated 5 out of 5 by from very effectiveOur lab analyzed SNX17 antibodies with WB, and we got all of them in 53kDa. In addition, the antibody can be reused.
Date published: 2017-10-26
Rated 5 out of 5 by from Clear band in WBWork well in WB of mouse cardiomyocytes and cardiac tissue!
Date published: 2017-06-28
Rated 5 out of 5 by from Good strong bandNice, strong band observed with SNX17 Antibody (H-10) in various samples
Date published: 2017-02-01
Rated 5 out of 5 by from Beautiful strong band in PCBeautiful strong band in PC-3 whole cell lysate. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Worked well with mouse and human SNX17 transfectedWorked well with mouse and human SNX17 transfected lysates by WB -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Great monoclonal antibodyGreat monoclonal antibody, good specificity and strong signal strength in HeLa, BJAB, A-431, K-562, Jurkat and MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-13
Rated 5 out of 5 by from WB: Strong band observed using ESWB: Strong band observed using ES-2 whole cell lysate. -SCBT QC
Date published: 2013-09-03
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SNX17 抗体 (H-10) is rated 5.0 out of 5 by 7.
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