Date published: 2026-10-17
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Smooth Muscle Actin Antibody (CGA7) - Western Blotting - Image 398872
平滑肌肌动蛋白抗体 (CGA7): sc-53015. C2C12 (A), Sol8 (B) 和 A-10 (C) 全细胞裂解液及人平滑肌组织提取物 (D) 中平滑肌肌动蛋白表达的 Western 印迹分析. 所用检测试剂: m-IgG Fc BP-HRP: sc-525409.
平滑肌肌动蛋白抗体 (CGA7): sc-53015. C2C12 (A), Sol8 (B) 和 A-10 (C) 全细胞裂解液及人平滑肌组织提取物 (D) 中平滑肌肌动蛋白表达的 Western 印迹分析. 所用检测试剂: m-IgG Fc BP-HRP: sc-525409.

Smooth Muscle Actin 抗体 (CGA7): sc-53015

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说明书
  • Smooth Muscle Actin抗体(CGA7)是小鼠单克隆IgG2b κ, 在130篇文献中引用，规格为200 µg/ml
  • 针对全长肫肌动蛋白（chicken来源）
  • Smooth Muscle Actin 抗体 (CGA7) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 avian来源的α and γ Actin isotypes of smooth muscle cells; 不会与 skeletal or cardiac cells产生交联反应
  • 抗Smooth Muscle Actin抗体（CGA7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是Smooth Muscle Actin Antibody (CGA7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与Smooth Muscle Actin Antibody (CGA7) 打包提供（请参阅下面的订购信息）。
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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Actin抗体，包括α-Actin, β-Actin, γ-Actin, Muscle Actin和Smooth Muscle Actin

平滑肌肌动蛋白抗体（CGA7）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人体组织中的平滑肌肌动蛋白。抗平滑肌肌动蛋白抗体（CGA7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。肌动蛋白作为细胞骨架的关键组成部分，在细胞结构和功能中发挥着至关重要的作用，为细胞提供机械支撑和形状。平滑肌肌动蛋白主要位于细胞质中，其形成的动态丝对细胞的各种过程至关重要，包括运动、分裂和细胞内运输。平滑肌肌动蛋白具有聚合和解聚的能力，使细胞能够根据外部刺激调整其形状和运动，这在平滑肌收缩和松弛中尤为重要。肌动蛋白的不同同种型，例如肌肉组织中的α-肌动蛋白和非肌肉细胞中的β-肌动蛋白，凸显了这种蛋白质在各种细胞环境中的多功能性和特殊性。了解平滑肌肌动蛋白的分布和功能对于研究肌肉生理学和病理学（包括血管疾病和平滑肌疾病）至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Smooth Muscle Actin 抗体 (CGA7) 参考文献:

  1. 表达α-平滑肌肌动蛋白的肌肉原代细胞的成骨潜能。  |  Matthews, BG., et al. 2016. Bone. 84: 69-77. PMID: 26721734
  2. 牙源性囊肿和肿瘤中α-平滑肌肌动蛋白的免疫组织化学研究。  |  Annegowda, VM., et al. 2018. J Oral Maxillofac Pathol. 22: 188-192. PMID: 30158770
  3. 糖尿病视网膜和脉络膜中α-平滑肌肌动蛋白阳性的血管周围细胞  |  Kim, SJ., et al. 2020. Int J Mol Sci. 21: PMID: 32245120
  4. 青春期前牛睾丸中α平滑肌肌动蛋白阳性管周细胞的特征。  |  Kawabe, Y., et al. 2022. Biochem Biophys Res Commun. 609: 48-53. PMID: 35413539
  5. 平滑肌肌动蛋白阳性皮肤梭形细胞鳞状细胞癌病例。  |  Sohng, C., et al. 2022. Ann Dermatol. 34: 230-232. PMID: 35721338
  6. α平滑肌肌动蛋白在牙髓干细胞牙源分化中的作用。  |  Ma, Z., et al. 2023. Eur J Oral Sci. 131: e12956. PMID: 37849216
  7. 浅表腹膜子宫内膜异位病变中平滑肌肌动蛋白和胶原的分布与周围微环境不同。  |  Colgrave, EM., et al. 2024. Reprod Biomed Online. 48: 103610. PMID: 38241767
  8. 牙周发育过程中成骨细胞分化中α-平滑肌肌动蛋白的定位。  |  Takebe, H., et al. 2024. Cell Tissue Res.. PMID: 39579220
  9. 探究α平滑肌肌动蛋白作为乳腺导管腺癌早期复发预测生物标志物的角色。  |  Palo, S. 2024. Iran J Pathol. 19: 363-364. PMID: 39687452
  10. 钙黏蛋白调节平滑肌肌动蛋白-肌球蛋白相互作用和力。  |  Winder, SJ., et al. 1998. Acta Physiol Scand. 164: 415-26. PMID: 9887965

订购信息

产品名称产品编号规格价格数量收藏夹

Smooth Muscle Actin 抗体 (CGA7)

sc-53015
200 µg/ml
$322.00

Smooth Muscle Actin (CGA7): m-IgG Fc BP-HRP 套装

sc-525569
200 µg Ab; 10 µg BP
$361.00

Smooth Muscle Actin (CGA7): m-IgG2b BP-HRP 套装

sc-549585
200 µg Ab; 10 µg BP
$361.00

Smooth Muscle Actin 抗体 (CGA7) AC

sc-53015 AC
500 µg/ml, 25% agarose
$424.00

Smooth Muscle Actin 抗体 (CGA7) HRP

sc-53015 HRP
200 µg/ml
$322.00

Smooth Muscle Actin 抗体 (CGA7) FITC

sc-53015 FITC
200 µg/ml
$336.00

Smooth Muscle Actin 抗体 (CGA7) PE

sc-53015 PE
200 µg/ml
$349.00

Smooth Muscle Actin 抗体 (CGA7) Alexa Fluor® 488

sc-53015 AF488
200 µg/ml
$364.00

Smooth Muscle Actin 抗体 (CGA7) Alexa Fluor® 546

sc-53015 AF546
200 µg/ml
$364.00

Smooth Muscle Actin 抗体 (CGA7) Alexa Fluor® 594

sc-53015 AF594
200 µg/ml
$364.00

Smooth Muscle Actin 抗体 (CGA7) Alexa Fluor® 647

sc-53015 AF647
200 µg/ml
$364.00

Smooth Muscle Actin 抗体 (CGA7) Alexa Fluor® 680

sc-53015 AF680
200 µg/ml
$364.00

Smooth Muscle Actin 抗体 (CGA7) Alexa Fluor® 790

sc-53015 AF790
200 µg/ml
$364.00

The protein is detected as a single band at a lower MW than in the data sheet. I also cross-checked with antibody from other company, and there is no problem with the sample. Is there a possibility of a 10-20kDa difference depending on the sample?

Asked by: abc12
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-11-14

Does SCBT sell a mouse antibody against smooth muscle actin gamma?

Asked by: Bladreg
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Is this antibody cross-reactive with skeletal or cardiac actin?

Asked by: AbPolly
Thank you for your inquiry. This antibody is specific to smooth muscle actin, and is not cross-reactive with skeletal or cardiac cells.
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by Purva from Expression of alpha-SMA in osteoporotic boneWe bought alpha-SMA and it has worked best as a fibrosis marker in bone regeneration studies
Date published: 2024-06-12
Rated 3 out of 5 by mariat from Good for IFGood antibody for immunofluorescence of human primary fibroblasts. Did not work for WB in the same samples.
Date published: 2022-06-07
Rated 5 out of 5 by rot0823 from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-10-21
Rated 5 out of 5 by poohey from Good for WBIt is useful for western blot analysis of human cell line.
Date published: 2020-09-04
Rated 5 out of 5 by Cinzia from Good antibodyIt worked very well in WB on human cancer associated fibroblasts ( 1:200 dilution).
Date published: 2018-11-05
Rated 1 out of 5 by cinncia from Not well work in WBToo many non-specific bands detected in human liver cancer BEL-7404. Dilution ratio is1:100
Date published: 2017-10-11
Rated 5 out of 5 by lis_dc from Used in WBIt was used for WB on rat sample, very roubust band at dilution 1:1000.
Date published: 2017-05-10
Rated 5 out of 5 by JHJia from SpecificStrong band in western blot analysis of Smooth Muscle Actin expression in A-10 whole cell lysate.
Date published: 2017-05-07
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Smooth Muscle Actin 抗体 (CGA7) is rated 4.6 out of 5 by 16.
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