SMN Antibody (11F3): sc-81554

SMN Antibody (11F3) is a mouse monoclonal IgG1 antibody that detects SMN in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). SMN, or survival of motor neuron, is a crucial protein involved in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), which are essential for pre-mRNA splicing. This process is vital for proper gene expression, ensuring introns are removed and exons are joined to form mature mRNA. SMN exists in multiple isoforms due to alternative splicing and forms a complex with several other proteins, including Gemin2, Gemin3, and various spliceosomal snRNP proteins, facilitating component assembly in the cytoplasm and recycling in the nucleus. SMN′s presence in both cytoplasm and nucleus, particularly in subnuclear structures known as gems, underscores SMN′s importance in cellular function and gene regulation. Mutations in the SMN gene are linked to spinal muscular atrophy, a severe neurodegenerative disease characterized by motor neuron loss, highlighting SMN′s critical role in neuronal health and function. With detection capabilities across various species and applications, anti-SMN antibody (11F3) serves as an invaluable tool for researchers studying molecular mechanisms underlying splicing and related disorders.

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SMN Antibody (11F3) References:

1. SMN regulation in SMA and in response to stress: new paradigms and therapeutic possibilities.  |  Dominguez, CE., et al. 2017. Hum Genet. 136: 1173-1191. PMID: 28852871
2. The Need for SMN-Independent Treatments of Spinal Muscular Atrophy (SMA) to Complement SMN-Enhancing Drugs.  |  Hensel, N., et al. 2020. Front Neurol. 11: 45. PMID: 32117013
3. Sumoylation regulates the assembly and activity of the SMN complex.  |  Riboldi, GM., et al. 2021. Nat Commun. 12: 5040. PMID: 34413305
4. SMN-deficient cells exhibit increased ribosomal DNA damage.  |  Karyka, E., et al. 2022. Life Sci Alliance. 5: PMID: 35440492
5. SMN controls neuromuscular junction integrity through U7 snRNP.  |  Tisdale, S., et al. 2022. Cell Rep. 40: 111393. PMID: 36130491
6. SMN promotes mitochondrial metabolic maturation during myogenesis by regulating the MYOD-miRNA axis.  |  Ikenaka, A., et al. 2023. Life Sci Alliance. 6: PMID: 36604149
7. The SMN Complex at the Crossroad between RNA Metabolism and Neurodegeneration.  |  Faravelli, I., et al. 2023. Int J Mol Sci. 24: PMID: 36768569
8. SMN linked to SNARE complex assembly.  |  Whalley, K. 2023. Nat Rev Neurosci. 24: 267. PMID: 36932212
9. Depletion of SMN protein in mesenchymal progenitors impairs the development of bone and neuromuscular junction in spinal muscular atrophy.  |  Hann, SH., et al. 2024. Elife. 12: PMID: 38318851
10. Arginine methylation-enabled FUS phase separation with SMN contributes to neuronal granule formation.  |  Wang, L. and Li, P. 2024. Cell Rep. 43: 114537. PMID: 39052476

The preferred SMN antibody is SMN Antibody (2B1): sc-32313.