Shc Antibody (H-11): sc-398289
- Shc Antibody (H-11) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 366-473 of Shc of human origin
- recommended for detection of Shc p66, p52 and p46 of mouse, rat and human origin by WB, IP, IF and ELISA
- See Shc (PG-797): sc-967 for Shc antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Shc Antibody (H-11) for WB applications. These reagents are now offered in bundles with Shc Antibody (H-11) (see ordering information below).
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Shc Antibody (H-11) is a mouse monoclonal IgG2a antibody that detects Shc protein in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Shc antibody (H-11) plays a crucial role in the signaling pathways initiated by growth factor receptor activation, which is essential for cellular responses such as proliferation and differentiation. Shc protein is primarily located in the cytoplasm, where Shc acts as an adapter that links activated receptor tyrosine kinases to downstream signaling molecules, thereby facilitating the transmission of signals to the nucleus. This localization allows Shc to rapidly respond to extracellular signals and initiate a cascade of intracellular events that lead to mitogenic responses. Additionally, Shc interacts with other key proteins, including GRB2 and Sos, which are critical for the activation of the Ras signaling pathway, further underscoring Shc′s importance in cellular signaling networks. The formation of complexes between Shc and these proteins enhances the specificity and efficiency of signal transduction, making Shc a pivotal player in the regulation of various biological processes.
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Shc Antibody (H-11) References:
- The role of adapter protein Shc in estrogen non-genomic action. | Zhang, Z., et al. 2004. Steroids. 69: 523-9. PMID: 15288764
- p66(shc) is highly expressed in fibroblasts from centenarians. | Pandolfi, S., et al. 2005. Mech Ageing Dev. 126: 839-44. PMID: 15992607
- Lck couples Shc to TCR signaling. | Fukushima, A., et al. 2006. Cell Signal. 18: 1182-9. PMID: 16257509
- A novel role of Shc adaptor proteins in steroid hormone-regulated cancers. | Alam, SM., et al. 2009. Endocr Relat Cancer. 16: 1-16. PMID: 19001530
- Mitochondrial pathways for ROS formation and myocardial injury: the relevance of p66(Shc) and monoamine oxidase. | Di Lisa, F., et al. 2009. Basic Res Cardiol. 104: 131-9. PMID: 19242637
- p66(Shc) restrains Ras hyperactivation and suppresses metastatic behavior. | Ma, Z., et al. 2010. Oncogene. 29: 5559-67. PMID: 20676142
- p66(Shc)-induced redox changes drive endothelial insulin resistance. | Paneni, F., et al. 2014. Atherosclerosis. 236: 426-9. PMID: 25150941
- p66Shc Couples Mechanical Signals to RhoA through Focal Adhesion Kinase-Dependent Recruitment of p115-RhoGEF and GEF-H1. | Wu, RF., et al. 2016. Mol Cell Biol. 36: 2824-2837. PMID: 27573018
- Human SHC-transforming protein 1 and its isoforms p66shc: A novel marker for prediabetes. | Jelinek, HF., et al. 2021. J Diabetes Investig. 12: 1881-1889. PMID: 33759377
- Emerging roles for SH2/PTB-containing Shc adaptor proteins in the developing mammalian brain. | Cattaneo, E. and Pelicci, PG. 1998. Trends Neurosci. 21: 476-81. PMID: 9829689
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Shc Antibody (H-11) | sc-398289 | 200 µg/ml | $322.00 | |||
Shc Antibody (H-11): m-IgG Fc BP-HRP Bundle | sc-538412 | 200 µg Ab; 10 µg BP | $361.00 | |||
Shc Antibody (H-11): m-IgGκ BP-HRP Bundle | sc-536021 | 200 µg Ab; 40 µg BP | $361.00 | |||
Shc Antibody (H-11): m-IgG2a BP-HRP Bundle | sc-548293 | 200 µg Ab; 10 µg BP | $361.00 |