Date published: 2025-9-7
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SH2-B α/β/γ/δ Antibody (46) - Western Blotting - Image 295735
SH2-B α/β/γ/δ 抗体 (46): sc-136065. SJRH30 (A), ES-2 (B) 和 OV-90 (C) 细胞全裂解液中 SH2-B α/β/γ/δ 表达的 Western 印迹分析.
SH2-B α/β/γ/δ Antibody (46) - Western Blotting - Image 295735
SH2-B α/β/γ/δ 抗体 (46): sc-136065. SJRH30 (A), ES-2 (B) 和 OV-90 (C) 细胞全裂解液中 SH2-B α/β/γ/δ 表达的 Western 印迹分析.
SH2-B α/β/γ/δ Antibody (46) - Immunofluorescence - Image 60683
SH2-B α/β/γ/δ (46): sc-136065. 对 HeLa 细胞进行免疫荧光染色, 显示细胞核和细胞质定位.
SH2-B α/β/γ/δ Antibody (46) - Western Blotting - Image 60512
SH2-B a/b/g/d (46): sc-136065. SW-13 全细胞裂解液中 SH2-B a/b/g/d 表达的 Western 印迹分析.
SH2-B α/β/γ/δ 抗体 (46): sc-136065. SJRH30 (A), ES-2 (B) 和 OV-90 (C) 细胞全裂解液中 SH2-B α/β/γ/δ 表达的 Western 印迹分析.

SH2-B α/β/γ/δ 抗体 (46): sc-136065

5.0(3)
寫評論提問

说明书
  • SH2-B alpha / beta / gamma / delta 抗体 46 是小鼠单克隆 IgG1, 在6篇文献中引用，提供 50 µg/0.5 ml
  • 免疫rat物种的SH2-Bβ的氨基酸403-513
  • 推荐用于 mouse, rat 和 human 来源的SH2-B α, β, γ and δ WB, IP 和 IF检测
  • 目前，我们还没有完成SH2-B α/β/γ/δ 抗体 (46)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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SH2-B，也被称为SH2B1或PSM，是一种由756个氨基酸组成的蛋白质，是信号网络的一个组成部分，参与细胞形态和运动的调节。SH2-B与APS（含有PH和SH2结构域的适配分子）家族的适配蛋白相关，这类蛋白的特征是含有一个血小板同源（PH）结构域、一个SH2结构域和一个酪氨酸磷酸化位点。SH2-B通过选择性剪接产生三种不同的异构体，即SH2-B α、β和γ，它们具有相同的N端序列，包括PH结构域、SH2结构域和多个富含脯氨酸的模体。由于含有一个PH结构域和一个SH2结构域，SH2-B在细胞核和细胞质之间穿梭。SH2-B广泛表达，在骨骼肌和卵巢中的表达水平最高。SH2-B在酪氨酸残基上磷酸化以响应受体激酶的刺激。

仅限研究使用。不适用于诊断和治疗用途。

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SH2-B α/β/γ/δ 抗体 (46)

sc-136065
50 µg/0.5 ml
$316.00

I am using SH2-B α/β/γ/δ (46): sc-136065 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Immunoblots endogenous SH2B1 in PC12 cellsAnti-SH2B1 (46) against aa 403-513 of rat SH2B1 (sc. 136065) PC12 cells stably expressing GFP tag (only endogenous SH2B1 present). Treated ± NGF (100 ng/ml), cells lysed in RIPA buffer (1% Triton X100). Load 40 ul on minigel. Immunoblot: block with 3% BSA in TBS-1% tween, blot with anti-SH2B1 (46) (1/250 dilution). 2o antibody: mIGGkBP-HRP (sc) (1/1000). Nice SH2B1 signal detected with NGF-dependent shift in migration. Band at 90 kDa in absence of NGF. Band at 100 kDa in sample treated with NGF. 293T cell transfected with the four isoforms of human GFP-SH2B1. Cells lysed in RIPA buffer (1% Triton X100). Load 20 ul on minigel. Immunoblot: block with 3% BSA in TBS-1% tween, blot with anti-SH2B1 (46) (1/1000 dilution). 2o antibody: anti-mouse IRdye680 (1/20,000). The four isoforms of SH2B1 were detected by the SH2B1 (46) antibody. For most antibodies prepared against SH2B1, the background is diminished when a 2o antibody conjugated to HRP is used (when compared to 2o antibodies conjugated to IRdyes.)
Date published: 2018-09-12
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear and cytoplasmic staining in HeLa cells. -SCBT QC
Date published: 2015-03-23
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SH2-B α/β/γ/δ 抗体 (46) is rated 5.0 out of 5 by 3.
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