SH-PTP2抗体 (B-1) 推荐用于 mouse, rat, human 和 avian 来源的SH-PTP2 WB, IP, IF, IHC(P), FCM 和 ELISA检测

SH-PTP2 抗体 (B-1): sc-7384

SH-PTP2抗体 (B-1) is rated 4.9 out of 5 by 25.
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说明书

    • 抗-SH-PTP2 抗体 B-1 是小鼠单克隆 IgG1 (kappa轻链),SH-PTP2抗体,规格为200 µg/ml
    • 特异性抗原位于human物种的SH-PTP2的C-terminus的氨基酸580-597之间
    • SH-PTP2 抗体 (B-1) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA ,检测 mouse, rat, human 和 avian来源的SH-PTP2; 也和以下物种反应,包括: equine and avian
    • 抗-SH-PTP2 抗体 (B-1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取SH-PTP2 (B-1): sc-7384免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

SH-PTP2 Antibody (B-1): sc-7384 in PD-1 Signaling Staining. Is this antibody conjugated with Fluorophore suit for detecting PD-1 signaling/function by Flow Cytometry? Then should I use fixation/permeabilization method? Would you mind provide a protocol

Asked by: Anonymous
Thank you for your question. SH-PTP2 Antibody (B-1): sc-7384 and its corresponding conjugated forms are reommended for detection of SH-PTP2 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P), FCM and ELISA. You can find our recommended protocol by following the link below: https://www.scbt.com/scbt/resources/protocols/flow-cytometry Feel free to also contact our technical service directly if you require any further information.
Answered by: Tech Support Europe
Date published: 2019-07-04

Can SH-PTP2 (B-1): sc-7384 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. SH-PTP2 (B-1): sc-7384 monoclonal antibody is currently available conjugated to PE, FITC, Alexa Fluor 488 or Alexa Fluor 647.
Answered by: Technical Support 13
Date published: 2018-07-01
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Rated 5 out of 5 by from Good for WB Very good for WB with human cell nuclear extract. I haven't try any other application yet.
Date published: 2020-07-10
Rated 3 out of 5 by from Disappointing performance in an IP reaction I bought this SH-PTP2 antibody to pulldown SHP2 from a cell lysate (overexpressor of SHP2 protein). I had previously done pulldowns with a different antibody. Photos attached. The Santa Cruz antibody gave a weaker pulldown and has a smear of protein showing up under the main 70kD band. Compare to the previous result. I'm a little disappointed.
Date published: 2019-11-26
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, MCF7, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 200 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 5 out of 5 by from Western blot This antibody had good expression in western blot. (cell line: HUVEC)
Date published: 2017-11-22
Rated 5 out of 5 by from wonderful antibody! 1:1000 diluted for wb of rat protein ,the band is strong and special.
Date published: 2017-08-28
Rated 5 out of 5 by from Function is very good Antibody is practical, the amount of antibody is relatively low
Date published: 2017-07-05
Rated 5 out of 5 by from this antibody works well in 1:1000 dilution WB This antibody works well in 1:1000 dilution WB. It's a good choice.
Date published: 2017-06-16
Rated 5 out of 5 by from Excellent antibody for westerns probed at 1000x dilution and used the kappa binding-HRP as secondary and the blot flawless.
Date published: 2017-03-09
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