Selenoprotein W CRISPR/Cas9 KO Plasmid (h): sc-405939
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Selenoprotein W CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Selenoprotein W genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Selenoprotein W CRISPR/Cas9 KO Plasmid (h) | sc-405939 | 20 µg | $397.00 |
Overview
SELENOW encodes Selenoprotein W, a small selenium-containing protein enriched in muscle and brain that contributes to cellular redox homeostasis through selenol-dependent antioxidant activity. It is implicated in regulating reactive oxygen species balance, maintaining protein thiol status, and supporting cytoskeletal integrity and stress adaptation under oxidative and inflammatory conditions. SELENOW-associated processes intersect with glutathione-dependent redox networks and broader selenium biology that influence mitochondrial function and cellular survival programs. Altered SELENOW expression has been reported in contexts of oxidative stress, neurodegeneration-associated pathways, and metabolic and inflammatory states, making it relevant for mechanistic studies of redox signaling and stress susceptibility.
Selenoprotein W CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SELENOW gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SELENOW together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SELENOW open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Selenoprotein W protein expression.
This CRISPR knockout system enables efficient generation of SELENOW-deficient cell models for investigation of Selenoprotein W signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SELENOW exon(s) critical for Selenoprotein W function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SELENOW genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Selenoprotein W CRISPR/Cas9 KO Plasmid (h) and Selenoprotein W CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SELENOW locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Selenoprotein W HDR Plasmid (h) and Selenoprotein W HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SELENOW homology arms to support homology-directed repair at defined SELENOW target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.