Date published: 2026-10-19
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Scrib Antibody (C-6) - Western Blotting - Image 361998
Scrib 抗体 (C-6): sc-55543. HeLa (A) 和 MCF7 (B) 全细胞裂解液中 Scrib 表达的 Western 印迹分析.
Scrib 抗体 (C-6): sc-55543. HeLa (A) 和 MCF7 (B) 全细胞裂解液中 Scrib 表达的 Western 印迹分析.

Scrib 抗体 (C-6): sc-55543

3.1(8)
寫評論提問
说明书
  • Scrib 抗体 C-6 是小鼠单克隆 IgG2b κ，Scrib抗体, 在11篇文献中引用，规格为200 µg/ml
  • 与氨基酸1331-1630 deletion 1444-1504（缺失1444-1504）的起源在Scrib的human的C-terminus
  • Scrib 抗体 (C-6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Scrib
  • 抗Scrib抗体（C-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是Scrib Antibody (C-6) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂，这些试剂现与Scrib Antibody (C-6) 打包提供（请参阅下面的订购信息）。
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描述
基因信息
说明书与实验方案
研究信息

関連項目

Scrib 抗体（C-6）是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附试验（ELISA）检测人源 Scrib 蛋白。抗 Scrib 抗体 (C-6) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Scrib 在维持上皮细胞完整性和调节细胞极性方面起着至关重要的作用，而细胞极性对正常的组织结构和功能至关重要。Scrib (C-6) 单克隆抗体定位在脊椎动物细胞的紧密连接处，Scrib 在那里与其他蛋白相互作用，确保粘连接头的正确位置，从而影响细胞粘附和信号传导途径。这种定位对形成功能性上皮屏障至关重要，上皮屏障可保护组织免受病原体侵害并调节分子通道。Scrib 的 PDZ 结构域与目标蛋白 C 端的特定序列结合，促进关键上皮决定因子的定位，并有助于整体细胞组织。Scrib 基因突变可导致严重的发育缺陷并导致肿瘤发生，这凸显了 Scrib 在正常生理和疾病状态中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Scrib 抗体 (C-6) 参考文献:

  1. 基底侧 PDZ 蛋白 Scribble 对顶端上皮决定因子的定位。  |  Bilder, D. and Perrimon, N. 2000. Nature. 403: 676-80. PMID: 10688207
  2. 黑腹果蝇的基因组序列。  |  Adams, MD., et al. 2000. Science. 287: 2185-95. PMID: 10731132
  3. PDZ 蛋白 Scribble 对突触可塑性和突触小泡动力学的调控。  |  Roche, JP., et al. 2002. J Neurosci. 22: 6471-9. PMID: 12151526
  4. Dlg, Scrib和Lgl调节神经母细胞的大小和有丝分裂纺锤体的不对称性。  |  Albertson, R. and Doe, CQ. 2003. Nat Cell Biol. 5: 166-70. PMID: 12545176
  5. 果蝇胚胎新形成性腺中 scribble 表达的要求。  |  Marhold, J., et al. 2003. Gene Expr Patterns. 3: 143-6. PMID: 12711540
  6. 星形胶质细胞迁移过程中，Scrib控制Cdc42定位和活性以促进细胞极化。  |  Osmani, N., et al. 2006. Curr Biol. 16: 2395-405. PMID: 17081755
  7. 拉线：果蝇发育过程中的细胞周期调控。  |  Lehner, CF. 1991. Semin Cell Biol. 2: 223-31. PMID: 1842341
  8. 极性蛋白Scrib对于定向内皮细胞迁移至关重要。  |  Michaelis, UR., et al. 2013. Circ Res. 112: 924-34. PMID: 23362312
  9. 心室心肌的形态发生需要 Scrib:Rac1 的相互作用。  |  Boczonadi, V., et al. 2014. Cardiovasc Res. 104: 103-15. PMID: 25139745
  10. 不同的 PDZ 结构域识别独特的羧基末端基团。  |  Songyang, Z., et al. 1997. Science. 275: 73-7. PMID: 8974395
  11. 紧密连接  |  Balda, MS. and Matter, K. 1998. J Cell Sci. 111 (Pt 5): 541-7. PMID: 9454728
  12. RhoA 和 Rac1 小 GTP 酶对紧密连接的结构和功能调控。  |  Jou, TS., et al. 1998. J Cell Biol. 142: 101-15. PMID: 9660866

订购信息

产品名称产品编号规格价格数量收藏夹

Scrib 抗体 (C-6)

sc-55543
200 µg/ml
$322.00

Scrib (C-6): m-IgG Fc BP-HRP 套装

sc-525636
200 µg Ab; 10 µg BP
$361.00

Scrib (C-6): m-IgG2b BP-HRP 套装

sc-549601
200 µg Ab; 10 µg BP
$361.00

Scrib 抗体 (C-6) AC

sc-55543 AC
500 µg/ml, 25% agarose
$424.00

Scrib 抗体 (C-6) HRP

sc-55543 HRP
200 µg/ml
$322.00

Scrib 抗体 (C-6) FITC

sc-55543 FITC
200 µg/ml
$336.00

Scrib 抗体 (C-6) PE

sc-55543 PE
200 µg/ml
$349.00

Scrib 抗体 (C-6) Alexa Fluor® 488

sc-55543 AF488
200 µg/ml
$364.00

Scrib 抗体 (C-6) Alexa Fluor® 546

sc-55543 AF546
200 µg/ml
$364.00

Scrib 抗体 (C-6) Alexa Fluor® 594

sc-55543 AF594
200 µg/ml
$364.00

Scrib 抗体 (C-6) Alexa Fluor® 647

sc-55543 AF647
200 µg/ml
$364.00

Scrib 抗体 (C-6) Alexa Fluor® 680

sc-55543 AF680
200 µg/ml
$364.00

Scrib 抗体 (C-6) Alexa Fluor® 790

sc-55543 AF790
200 µg/ml
$364.00

We are working on gastric cancer and we would like to evaluate scrib membrane labeling. What type of human tissue can be used as a witness ? Thank you very much.

Asked by: marion jost
Thank you for your question. We recommend Hela cells, MCF-7 cells and T-47D cells as positive controls for the Scrib protein.
Answered by: Tech Service
Date published: 2019-06-20

Can Scrib (C-6): sc-55543 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Scrib (C-6): sc-55543 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by Annas9n from Didn't work in mouse IHC(Fr) or WBWe tried this antibody on sections of mouse embryo and on westerns of mouse embryo lysates, and got no specific signal.
Date published: 2019-06-03
Rated 1 out of 5 by Jamesgjh from Not Good for IFIn Hek293, we did not detect any specific Immunofluorescence signal. Tried twice!
Date published: 2017-11-29
Rated 3 out of 5 by jia626 from WB worksWB only works in human tissue, doesn't work in mouse tissue
Date published: 2017-08-23
Rated 5 out of 5 by wangyyy from Nice for western blotWorks well in 293A cells, and I got a clean band when incubated the membrane with 1:1000 dilution of primary antibody at 4°C overnight
Date published: 2017-07-25
Rated 1 out of 5 by kmdg from Didn't workSome non-specific bands at lower molecular weights, but nothing at the proper molecular weight when used in a western on NRVMs.
Date published: 2017-06-16
Rated 4 out of 5 by TO from Robust bands seen in WB using HeLa and JurkatRobust bands seen in WB using HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-06-30
Rated 5 out of 5 by FL from WB publishable data with human Jurkat T cellsWB publishable data with human Jurkat T cells - SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by NZ from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2013-12-03
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Scrib抗体 (C-6) is rated 3.1 out of 5 by 8.
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