Date published: 2025-9-7
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SCD Antibody (CD.E10) - Western Blotting - Image 368572
SCD 抗体 (CD. E10): sc-58420. SK-MEL-28 (A) 和 Hep G2 (B) 全细胞裂解液中 SCD 表达的 Western 印迹分析.
SCD Antibody (CD.E10) - Western Blotting - Image 368572
SCD 抗体 (CD. E10): sc-58420. SK-MEL-28 (A) 和 Hep G2 (B) 全细胞裂解液中 SCD 表达的 Western 印迹分析.
SCD 抗体 (CD. E10): sc-58420. SK-MEL-28 (A) 和 Hep G2 (B) 全细胞裂解液中 SCD 表达的 Western 印迹分析.

SCD 抗体 (CD.E10): sc-58420

3.7(3)
寫評論提問

说明书
  • SCD 抗体 CD.E10 是小鼠单克隆 IgG2b, 在14篇文献中引用，提供 100 µg/ml
  • 针对全长重组SCD，human起源
  • 推荐用于 SCD of human origin and SCD1, SCD2, SCD3 and SCD4 of mouse and rat origin WB, IP, IF 和 ELISA检测
  • 关于如何获取SCD (CD.E10): sc-58420的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是SCD Antibody (CD.E10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与SCD Antibody (CD.E10) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加SCD抗体，包括SCD1和SCD2

SCD抗体（CD.E10）是一种IgG2b小鼠单克隆SCD抗体，可通过蛋白质印迹法、免疫沉淀法、免疫荧光法和酶联免疫吸附测定法检测人类来源的SCD以及小鼠和大鼠来源的SCD1、SCD2、SCD3和SCD4。SCD抗体（CD.E10）为非偶联型抗SCD抗体。硬脂酰辅酶A去饱和酶(SCD)是一种微粒体酶，是合成油酸和棕榈油酸所必需的，油酸和棕榈油酸是膜磷脂、甘油三酯和胆固醇酯的主要单不饱和脂肪酸。SCD在三酰甘油和磷脂分泌过程以及细胞胆固醇稳态机制中发挥主要作用。它在细胞中迅速转化，因此是研究内质网短寿命蛋白选择性降解的模型。SCD也是膜流动性的重要调节因子。在诱导分化为脂肪细胞的细胞以及某些肿瘤细胞系中观察到SCD表达水平的增加。由于基因复制事件，SCD家族中的基因数量在不同物种之间有所不同。它们的表达模式受到动物饮食中不饱和脂肪酸水平的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SCD 抗体 (CD.E10) 参考文献:

  1. 硬脂酰-CoA 去饱和酶 1 基因中断的小鼠肝脏胆固醇酯和甘油三酯的生物合成受到影响。  |  Miyazaki, M., et al. 2000. J Biol Chem. 275: 30132-8. PMID: 10899171
  2. 微粒体 delta 9 硬脂酰-CoA 去饱和酶的 N 端含有决定其快速降解的序列。  |  Mziaut, H., et al. 2000. Proc Natl Acad Sci U S A. 97: 8883-8. PMID: 10922050
  3. 硬脂酰-CoA 去饱和酶 1 编码序列和反义 RNA 影响转染鸡 LMH 肝瘤细胞的脂质分泌。  |  Diot, C., et al. 2000. Arch Biochem Biophys. 380: 243-50. PMID: 10933878
  4. 噻唑烷二酮类对 3T3-L1 脂肪细胞中硬脂酰-CoA 去饱和酶基因的不同调控。  |  Kim, YC., et al. 2000. J Lipid Res. 41: 1310-6. PMID: 10946019
  5. 硬脂酰-CoA脱饱和酶（SCD）通过增强雄激素受体转激活促进前列腺癌细胞的增殖。  |  Kim, SJ., et al. 2011. Mol Cells. 31: 371-7. PMID: 21331774
  6. 3T3-L1 前脂肪细胞中分化诱导的基因表达。第二个差异表达基因编码硬脂酰-CoA 去饱和酶。  |  Kaestner, KH., et al. 1989. J Biol Chem. 264: 14755-61. PMID: 2570068
  7. 3T3-L1 前脂肪细胞分化诱导的基因表达。编码硬脂酰-CoA 去饱和酶的差异表达基因的特征。  |  Ntambi, JM., et al. 1988. J Biol Chem. 263: 17291-300. PMID: 2903162
  8. 硬脂酰-CoA脱饱和酶（SCD）的减少会导致慢性肾病中肌萎缩和内质网应激增加。  |  Niida, Y., et al. 2020. J Clin Biochem Nutr. 67: 179-187. PMID: 33041516
  9. 硬脂酰-CoA脱饱和酶（SCD）通过心脏脂质过载和血管紧张素II AT1受体蛋白上调诱导心脏功能障碍。  |  Abd Alla, J., et al. 2021. Int J Mol Sci. 22: PMID: 34576047
  10. 良性与恶性乳腺肿瘤周围脂肪组织中的脂肪酸谱和Delta9脱饱和酶（硬脂酰-CoA脱饱和酶；SCD 1）表达。  |  Sefidabi, R., et al. 2023. Heliyon. 9: e20658. PMID: 37885725
  11. 硬脂酰-CoA脱饱和酶（SCD）的调节。  |  Ntambi, JM. 1995. Prog Lipid Res. 34: 139-50. PMID: 7480063
  12. 人类硬脂酰-CoA 去饱和酶 cDNA 的部分特征及其在一些正常组织和恶性组织中的 mRNA 表达变化。  |  Li, J., et al. 1994. Int J Cancer. 57: 348-52. PMID: 7909540

订购信息

产品名称产品编号规格价格数量收藏夹

SCD 抗体 (CD.E10)

sc-58420
100 µg/ml
$316.00

SCD (CD.E10): m-IgG Fc BP-HRP 套装

sc-539081
100 µg Ab; 10 µg BP
$354.00

Is it recommended to detection of SCD1 of of human origin?

Asked by: Luna_BR
Thank you for your question. Yes, this monoclonal antibody is recommended for detection of SCD of human origin (also designated SCD1).
Answered by: Technical Support
Date published: 2017-12-02

For Western Blot, is it recommended to use denatured or non-denatured conditions with SCD (CD.E10): sc-58420 antibody?

Asked by: Cweed
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western BlotThis antibody has been used for western blotting in published research. -SCBT QC
Date published: 2023-09-14
Rated 2 out of 5 by from SCD antibodyRegarding the mouse monoclonal antibody raised against SCD, that was recommended for detection of SCD of mouse, rat and human origin by western Blotting, we did not achieve satisfactory results on different kinds of samples obtained from rat liver and HepG2 whole cell lysates. The antibody was incubated overnight at the dilutions of 1:1000 and 1:500 obtaining the same result shown in figure 1. The molecular weight of SCD is 40 KDa, but as can be seen in figure 1, we have not obtained a single recognizable band. We got a band between 75 and 50 KDa and another one at around 37 KDa. Besides identifying the correct height of the bands, another problem is that the bands do not appear in all the samples that we loaded in the gel, but their arrangement appears at least random.
Date published: 2020-01-16
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SCD 抗体 (CD.E10) is rated 3.7 out of 5 by 3.
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