S2P Antibody (1A3): sc-293341

S2P Antibody (1A3) is a mouse monoclonal IgG1 kappa light chain antibody that detects S2P protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-S2P antibody (1A3) is available as the non-conjugated form. S2P protein plays a crucial role in the proteolytic activation of sterol regulatory element binding proteins (SREBPs), which are essential transcription factors that regulate cholesterol metabolism. Located in the endoplasmic reticulum (ER), S2P is responsible for the second cleavage of SREBPs after the initial cleavage by Site-1 protease (S1P). This process allows the N-terminal domain of SREBPs to translocate to the nucleus, where S2P activates genes involved in lipid biosynthesis and cholesterol homeostasis. S2P activity regulation is tightly linked to cellular sterol levels, making S2P a key player in maintaining lipid balance within cells. S2P (1A3) antibody helps researchers gain insights into cholesterol metabolism mechanisms and SREBP roles in various physiological and pathological conditions.

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S2P Antibody (1A3) References:

1. Autocatalytic processing of site-1 protease removes propeptide and permits cleavage of sterol regulatory element-binding proteins.  |  Espenshade, PJ., et al. 1999. J Biol Chem. 274: 22795-804. PMID: 10428864
2. Secreted site-1 protease cleaves peptides corresponding to luminal loop of sterol regulatory element-binding proteins.  |  Cheng, D., et al. 1999. J Biol Chem. 274: 22805-12. PMID: 10428865
3. A proteolytic pathway that controls the cholesterol content of membranes, cells, and blood.  |  Brown, MS. and Goldstein, JL. 1999. Proc Natl Acad Sci U S A. 96: 11041-8. PMID: 10500120
4. Sterols regulate cycling of SREBP cleavage-activating protein (SCAP) between endoplasmic reticulum and Golgi.  |  Nohturfft, A., et al. 1999. Proc Natl Acad Sci U S A. 96: 11235-40. PMID: 10500160
5. Regulated cleavage of sterol regulatory element binding proteins requires sequences on both sides of the endoplasmic reticulum membrane.  |  Hua, X., et al. 1996. J Biol Chem. 271: 10379-84. PMID: 8626610
6. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies.  |  Sakai, J., et al. 1998. J Biol Chem. 273: 5785-93. PMID: 9488713
7. Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs.  |  Rawson, RB., et al. 1997. Mol Cell. 1: 47-57. PMID: 9659902
8. Molecular identification of the sterol-regulated luminal protease that cleaves SREBPs and controls lipid composition of animal cells.  |  Sakai, J., et al. 1998. Mol Cell. 2: 505-14. PMID: 9809072