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S-100 β chain Antibody (C-3) - Western Blotting - Image 299217
S-100 β 链 (C-3): sc-393919. 人重组 S100B 的 Western 印迹分析.
S-100 β 链 (C-3): sc-393919. 人重组 S100B 的 Western 印迹分析.

S-100 β chain 抗体 (C-3): sc-393919

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说明书
  • S-100 β chain抗体(C-3)是小鼠单克隆IgG2a κ, 在13篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的S-100 β chain的内部区域内的氨基酸34-48之间
  • 抗-S-100 beta chain 抗体 (C-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 S-100 β chain; 也和以下物种反应，包括： equine
  • 抗S-100 beta chain抗体（C-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取S-100 β chain (C-3): sc-393919的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 S-100 β chain 抗体 (C-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与S-100 β chain 抗体 (C-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 S-100 抗体包括 S-100 α chain, S-100 β chain, S-100A10, S-100A13, S-100A14, S-100A16, S-100A2, S-100A3 和 S-100P

S-100 β链抗体（C-3）是一种IgG2a κ小鼠单克隆S-100 β链抗体（也称为S100蛋白B（S100B）抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的S-100 β链。S-100 β链抗体（C-3）既可作为非偶联抗S-100 β链抗体形式，也可作为多种偶联形式的抗S-100 β链抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。EF手型Ca2+结合蛋白家族包括钙结合蛋白（以前称为维生素D依赖性Ca2+结合蛋白）、S-100 α和β、钙粒蛋白A（也称为MRP8）、B（也称为MRP14）和C（S-100样蛋白）以及小清蛋白家族成员，包括小清蛋白α和小清蛋白β（也称为oncomodulin）。S-100蛋白参与细胞过程的调节，如细胞周期进程和分化。研究还表明，S-100蛋白可能在Ca2+诱导的Ca2+释放的激活、微管组装的抑制和蛋白激酶C介导的磷酸化的抑制中发挥作用。两个共享60%序列同一性的S-100亚基已被描述为S-100 α链和S-100 β链。已经表征了三种S-100二聚体形式，它们在由两条α链、两条β链或一条α链和一条β链组成的亚基组成上有所不同。S-100定位于星形胶质细胞、施万细胞、室管膜瘤和星形胶质细胞瘤的细胞质和细胞核。S-100也几乎在所有良性痣、恶性黑素瘤和皮肤中的朗格汉斯细胞中检测到。钙结合蛋白、S-100蛋白和小清蛋白均在神经组织中表达。此外，S-100 α和β存在于多种其他组织中，而钙结合蛋白存在于肠道和肾脏中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

S-100 β chain 抗体 (C-3) 参考文献:

  1. [S-100beta 蛋白的病理生理学方面：脑病理学的新生物标志物]。  |  Beaudeux, J., et al. 1999. Ann Biol Clin (Paris). 57: 261-72. PMID: 10377476
  2. 大鼠 S100 alpha cDNA 的分离及其 mRNA 在大鼠组织中的分布。  |  Zimmer, DB., et al. 1991. Brain Res Bull. 27: 157-62. PMID: 1742602
  3. EF 型钙结合蛋白。  |  Heizmann, CW. 1988. J Cardiovasc Pharmacol. 12 Suppl 5: S30-7. PMID: 2469875
  4. 用两种不同的免疫测定法测定 S100B 蛋白浓度。  |  Feriel, J., et al. 2015. Clin Chem Lab Med. 53: e169-71. PMID: 25719325
  5. 小儿糖尿病酮症酸中毒的血清神经元特异性烯醇化酶和S100钙结合蛋白B  |  Elshorbagy, HH., et al. 2019. J Clin Res Pediatr Endocrinol. 11: 374-387. PMID: 31067852
  6. S100B及其与HIV相关神经认知障碍的关系  |  Limpruttidham, N., et al. 2019. J Neurovirol. 25: 899-900. PMID: 31278533
  7. 在无血清培养基中生长的下丘脑神经元中的副发光素和钙粘蛋白 D28K 的发育和功能研究  |  Pfyffer, GE., et al. 1987. J Neurochem. 49: 442-51. PMID: 3298546
  8. 大鼠睾丸发育过程中 Ca2+ 结合蛋白parvalbumin, 钙结合蛋白 D-28K, S-100 蛋白和钙调素的发育外观。  |  Kägi, U., et al. 1988. Cell Tissue Res. 252: 359-65. PMID: 3383215
  9. 通过直接注射副缬氨酸 cDNA 提高骨骼肌松弛速度  |  Müntener, M., et al. 1995. Proc Natl Acad Sci U S A. 92: 6504-8. PMID: 7604022
  10. 大鼠大脑神经元亚群中 S100 蛋白的表达。  |  Rickmann, M. and Wolff, JR. 1995. Neuroscience. 67: 977-91. PMID: 7675218
  11. 维甲酸调节钙结合蛋白钙宾蛋白-D28K的表达。  |  Wang, YZ. and Christakos, S. 1995. Mol Endocrinol. 9: 1510-21. PMID: 8584029
  12. 羊水中的一种新型钙结合蛋白 CAAF1：其分子克隆和组织分布。  |  Hitomi, J., et al. 1996. J Cell Sci. 109 (Pt 4): 805-15. PMID: 8718672

订购信息

产品名称产品编号规格价格数量收藏夹

S-100 β chain 抗体 (C-3)

sc-393919
200 µg/ml
$316.00

S-100 β chain (C-3): m-IgG Fc BP-HRP 套装

sc-530636
200 µg Ab; 10 µg BP
$354.00

S-100 β chain (C-3): m-IgGκ BP-HRP 套装

sc-524151
200 µg Ab, 40 µg BP
$354.00

S-100 β chain (C-3): m-IgG2a BP-HRP 套装

sc-547543
200 µg Ab; 10 µg BP
$354.00

S-100 β chain 抗体 (C-3) AC

sc-393919 AC
500 µg/ml, 25% agarose
$416.00

S-100 β chain 抗体 (C-3) HRP

sc-393919 HRP
200 µg/ml
$316.00

S-100 β chain 抗体 (C-3) FITC

sc-393919 FITC
200 µg/ml
$330.00

S-100 β chain 抗体 (C-3) PE

sc-393919 PE
200 µg/ml
$343.00

S-100 β chain 抗体 (C-3) Alexa Fluor® 488

sc-393919 AF488
200 µg/ml
$357.00

S-100 β chain 抗体 (C-3) Alexa Fluor® 546

sc-393919 AF546
200 µg/ml
$357.00

S-100 β chain 抗体 (C-3) Alexa Fluor® 594

sc-393919 AF594
200 µg/ml
$357.00

S-100 β chain 抗体 (C-3) Alexa Fluor® 647

sc-393919 AF647
200 µg/ml
$357.00

S-100 β chain 抗体 (C-3) Alexa Fluor® 680

sc-393919 AF680
200 µg/ml
$357.00

S-100 β chain 抗体 (C-3) Alexa Fluor® 790

sc-393919 AF790
200 µg/ml
$357.00

S-100 β chain (C-3) 中和勝肽

sc-393919 P
100 µg/0.5 ml
$68.00

Hello, I want to use S100B to label human cortical astrocytes in culture. Would S-100 β chain Antibody (C-3) work for ICC/IF my culture with astrocytes ? Also do you recommend other markers other than GFAP for astrocytes ?

Asked by: Dana 123
Thank you for your question. This monoclonal antibody, S-100 β chain (C-3) sc-393919, is human reactive and should be fine for IF and IHC use on human samples. We have not tested specifically on astrocytes, so I do not have any information specifically regarding that sample. We do not have information regarding optimal astrocyte markers, so I would refer you to the primary literature for that information.
Answered by: Tech Service
Date published: 2019-10-02

What application is the blocking peptide sc-393919 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Disappinting signalApplication: Immunofluorescence in (4% PFA fixed ) paraffin embedded sample of rat colon using heat mediated antigen retrieval with TRIS/EDTA buffer (pH:9, 20min, 92°C) or heat mediated antigen retrieval with citrate buffer (10min, 100°C) and a dilution of 1:50. Conclusion: the antibody shows immunoreactivity using antigen retrieval with TRIS/EDTA buffer only. In this preparation it labels the glial cells exclusively inside the ganglia (myenteric and submucous). Likely, not all the ganglia were labelled.
Date published: 2020-11-24
Rated 3 out of 5 by from Low signal in my samplesI have tried this antibody For WB on cytoplasmic fraction of PBMC and the signal was very poor
Date published: 2020-09-02
Rated 4 out of 5 by from Very good for IHCWe used 1:50 on adult mouse brain sections, it works great using both florescence secondary antibody and DAB.
Date published: 2018-07-19
Rated 5 out of 5 by from Good mAbIt worked as expected. I was glad to test the antibody in this free trial
Date published: 2017-12-18
Rated 5 out of 5 by from specific signal for WBI used this antibody for western blot in a 1:1000 dilution in lanes with 40 ug protein and got a very specific signal around 21kda.
Date published: 2017-08-12
Rated 3 out of 5 by from No changed result by adjust the conditionAccording to the datasheet ,there are two bands 10kda and 21kd.However, I got very weak band at 17kda,the 10kda is disappear.I tried different conditions, but no improvement,maybe the problem from the antibody quality.
Date published: 2017-07-15
Rated 1 out of 5 by from Did not work on mouse sciatic nerveThis antibody did not work on frozen mouse cryosection.
Date published: 2017-05-02
Rated 5 out of 5 by from Great antibodyS-100 beta chain Antibody (C-3) worked will by IHCP. Strong and robust staining observed. Also, worked well by Western blot
Date published: 2017-01-30
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S-100 β chain 抗体 (C-3) is rated 3.6 out of 5 by 9.
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