Date published: 2025-9-7

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RPA 70 kDa subunit 抗体 (B-6): sc-28304

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说明书
  • RPA 70 kDa subunit抗体(B-6)是小鼠单克隆IgG2a κ, 在26篇文献中引用,规格为200 µg/ml
  • 免疫human物种的RPA 70 kDa subunit的氨基酸317-616
  • 抗-RPA 70 kDa subunit 抗体 (B-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 RPA 70 kDa subunit
  • 抗RPA 70 kDa subunit抗体(B-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-28304 X, 200 µg/0.1 ml)
  • 关于如何获取RPA 70 kDa subunit (B-6): sc-28304免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 RPA 70 kDa subunit 抗体 (B-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与RPA 70 kDa subunit 抗体 (B-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

RPA 70 kDa 亚基抗体(B-6)是一种小鼠单克隆 IgG2a kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的 RPA 70 kDa 亚基。RPA 70 kDa亚基抗体(B-6)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。RPA 70 kDa亚基是复制蛋白A(RPA)复合物的重要组成部分,对于真核细胞的DNA复制、重组和修复至关重要。RPA 70 kDa亚基主要位于细胞核中,在DNA损伤反应和复制过程中与单链DNA(ssDNA)结合。RPA 70 kDa亚基的核定位使其能够立即进入需要修复或复制的ssDNA区域,从而促进其他修复蛋白的招募并确保基因组的稳定性。RPA 70 kDa亚基与各种细胞和病毒蛋白的相互作用协调了DNA代谢的多个途径。RPA 70 kDa亚基的结构包括一个DNA结合结构域,该结构域由两个同源亚结构域组成,对于与单链DNA形成稳定的复合物至关重要,从而防止形成可能阻碍复制和修复过程的二级结构。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RPA 70 kDa subunit 抗体 (B-6) 参考文献:

  1. 与 RFA1 基因相互作用的特征:RPA 在 DNA 复制和端粒维持中的作用。  |  Smith, J., et al. 2000. Biochimie. 82: 71-8. PMID: 10717390
  2. RPA 通过蛋白质与蛋白质之间的相互作用,稳定 XPA 破坏的 DNA 复合物。  |  Wang, M., et al. 2000. Biochemistry. 39: 6433-9. PMID: 10828957
  3. 复制蛋白 a 32 kDa 亚基(RPA p32)与 STAT3 的 SH2 结构域结合,并调节其转录活性。  |  Kim, J., et al. 2000. Cell Biol Int. 24: 467-73. PMID: 10875894
  4. 人类复制蛋白 A(hRPA)与伪原 DNA 底物结合的 5' --> 3' 分子极性。  |  Iftode, C. and Borowiec, JA. 2000. Biochemistry. 39: 11970-81. PMID: 11009611
  5. 氧化还原调控 RPA-ssDNA 相互作用过程中锌指图案的功能特征。  |  You, JS., et al. 2000. Biochemistry. 39: 12953-8. PMID: 11041860
  6. 复制因子 RPA 识别 DNA 修复蛋白 UNG2, XPA 和 RAD52 的结构基础。  |  Mer, G., et al. 2000. Cell. 103: 449-56. PMID: 11081631
  7. 复制蛋白 A(RPA)70-kDa 亚基 N 端终止子的调控功能。  |  Binz, SK. and Wold, MS. 2008. J Biol Chem. 283: 21559-70. PMID: 18515800
  8. 编码人类复制蛋白 A 的 70 kDa 单链 DNA 结合亚基的 cDNA 的特征及其在 DNA 复制中的作用。  |  Erdile, LF., et al. 1991. J Biol Chem. 266: 12090-8. PMID: 2050703
  9. 人类复制蛋白 A 32 kDa 亚基的主要结构。  |  Erdile, LF., et al. 1990. J Biol Chem. 265: 3177-82. PMID: 2406247
  10. Rpa4,复制蛋白 A 复合物 34 千道尔顿亚基的同源物。  |  Keshav, KF., et al. 1995. Mol Cell Biol. 15: 3119-28. PMID: 7760808
  11. 人类复制蛋白 A 的结构分析:绘制 70 kDa 亚基的功能域图。  |  Gomes, XV. and Wold, MS. 1995. J Biol Chem. 270: 4534-43. PMID: 7876222
  12. 与 DNA 结合的复制蛋白 A 单链 DNA 结合域的结构。  |  Bochkarev, A., et al. 1997. Nature. 385: 176-81. PMID: 8990123

订购信息

产品名称产品编号规格价格数量收藏夹

RPA 70 kDa subunit 抗体 (B-6)

sc-28304
200 µg/ml
$316.00

RPA 70 kDa subunit (B-6): m-IgG Fc BP-HRP 套装

sc-528414
200 µg Ab; 10 µg BP
$354.00

RPA 70 kDa subunit (B-6): m-IgGκ BP-HRP 套装

sc-520788
200 µg Ab, 40 µg BP
$354.00

RPA 70 kDa subunit (B-6): m-IgG2a BP-HRP 套装

sc-547089
200 µg Ab; 10 µg BP
$354.00

RPA 70 kDa subunit 抗体 (B-6) X

sc-28304 X
200 µg/0.1 ml
$316.00

RPA 70 kDa subunit 抗体 (B-6) AC

sc-28304 AC
500 µg/ml, 25% agarose
$416.00

RPA 70 kDa subunit 抗体 (B-6) HRP

sc-28304 HRP
200 µg/ml
$316.00

RPA 70 kDa subunit 抗体 (B-6) FITC

sc-28304 FITC
200 µg/ml
$330.00

RPA 70 kDa subunit 抗体 (B-6) PE

sc-28304 PE
200 µg/ml
$343.00

RPA 70 kDa subunit 抗体 (B-6) Alexa Fluor® 488

sc-28304 AF488
200 µg/ml
$357.00

RPA 70 kDa subunit 抗体 (B-6) Alexa Fluor® 546

sc-28304 AF546
200 µg/ml
$357.00

RPA 70 kDa subunit 抗体 (B-6) Alexa Fluor® 594

sc-28304 AF594
200 µg/ml
$357.00

RPA 70 kDa subunit 抗体 (B-6) Alexa Fluor® 647

sc-28304 AF647
200 µg/ml
$357.00

RPA 70 kDa subunit 抗体 (B-6) Alexa Fluor® 680

sc-28304 AF680
200 µg/ml
$357.00

RPA 70 kDa subunit 抗体 (B-6) Alexa Fluor® 790

sc-28304 AF790
200 µg/ml
$357.00

We hqve the lot# H2304. Do you consider it is still stable? I think the antibody has spend more than one year at 4C. Thank you so much for your help

Asked by: msjb
Thank you for your question. We provide a 12-month warranty on purchased products. If kept at its recommended storage temperature of 4 °C for one year the antibody should still be perfectly usable. Antibodies may still be stable after years of appropriate storage. If the antibody produces weak or unspecific signals or no signal at all we would recommend using a new vial.
Answered by: Tech Support Europe
Date published: 2020-07-14

When should the TransCruz reagent sc-28304 X be used instead of the standard form sc-28304?

Asked by: jerojero
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Provides great resultsThis antibody provides clear and specific data in blotting and staining that can be used for publishing. It is mouse, rat, and human reactive and has been cited in several publications.
Date published: 2017-02-02
Rated 4 out of 5 by from Strong bands seen in WB using HeLa and NIH/3T3Strong bands seen in WB using HeLa and NIH/3T3 nuclear extracts. -SCBT QC
Date published: 2015-06-06
Rated 4 out of 5 by from Publishable data in IP using human 293T cellsPublishable data in IP using human 293T cells. -SCBT Publication Review
Date published: 2015-05-21
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nucelarProduced exceptional immunoperoxidase nucelar and cytoplasmic staining in formalin fixed, paraffin-embedded human cervix tissue. -SCBT QC
Date published: 2015-04-02
Rated 4 out of 5 by from Various publishable data in WB using humanVarious publishable data in WB using human BL21 (DE3) cells, fibroblasts, MCF-7, PC3 and LLC cells. -SCBT Publication Review
Date published: 2015-01-14
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-01-14
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RPA 70 kDa subunit 抗体 (B-6) is rated 4.3 out of 5 by 6.
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