RP105 Double Nickase Plasmid (h): sc-409643-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RP105 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RP105 Double Nickase Plasmid (h) and RP105 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD180. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RP105 Antibody (MHR73): sc-52721
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RP105 Double Nickase Plasmid (h) | sc-409643-NIC | 20 µg | $410.00 | |||
RP105 Double Nickase Plasmid (h2) | sc-409643-NIC-2 | 20 µg | $410.00 |
Human CD180 encodes RP105, a Toll-like receptor family member that complexes with MD-1 and functions as a regulatory component of innate immune signaling in B cells and antigen-presenting cells. RP105 modulates responses to lipopolysaccharide and other microbial cues, shaping downstream NF-κB and MAPK pathway activity that influences cytokine production, proliferation, and survival. Through its role in controlling TLR4-associated signaling thresholds and B-cell activation states, CD180 is relevant to studies of inflammatory regulation and immune dysregulation observed across autoimmunity, infection biology, and B-cell–driven malignancy contexts. Altered RP105 expression and signaling balance has been associated with aberrant immune activation and changes in humoral immune responses, making it a useful node for mechanistic interrogation of innate-adaptive crosstalk.
RP105 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD180 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD180. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD180 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD180-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.