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Rotavirus capsid 抗体 (2B4): sc-101363

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说明书
  • Rotavirus capsid抗体(2B4)是小鼠单克隆IgG2b κ, 在11篇文献中引用,规格为50 µg/0.5 ml
  • 针对重组Rotavirus capsid
  • 推荐用于 Rotavirus 来源的inner capsid protein VP6 WB, IF, IHC(P) 和 ELISA检测
  • 关于如何获取Rotavirus capsid (2B4): sc-101363免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Rotavirus capsid 抗体 (2B4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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轮状病毒衣壳抗体(2B4)是一种IgG2b κ小鼠单克隆轮状病毒衣壳抗体,可通过WB、IF、IHC(P)和ELISA检测轮状病毒的内衣壳蛋白VP6。轮状病毒衣壳抗体(2B4)以非偶联抗轮状病毒衣壳抗体的形式存在。轮状病毒,呼肠孤病毒科的一个属,是双链RNA病毒,是婴幼儿胃肠炎的主要病因之一。该病毒存在七种,分别被命名为轮状病毒A、B、C、D、E、F和G,其中轮状病毒A是最常见的感染原因。轮状病毒通过粪口途径传播,一旦进入人体,就会感染小肠细胞,产生一种肠毒素,改变细胞壁的通透性,引起严重腹泻和脱水。轮状病毒进入细胞的方式可以是直接穿透细胞膜,也可以是内吞作用后膜泡溶解,这两种方式都由轮状病毒衣壳促进。轮状病毒衣壳由三层同心蛋白层组成,其中外两层被称为VP4和VP7,在病毒进入细胞的过程中以钙依赖的方式被牺牲。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rotavirus capsid 抗体 (2B4) 参考文献:

  1. 轮状病毒荚膜蛋白 VP5* 可使膜渗透。  |  Denisova, E., et al. 1999. J Virol. 73: 3147-53. PMID: 10074166
  2. 轮状病毒外囊糖蛋白 VP7 抗体通过抑制病毒的脱帽作用中和了病毒的感染性。  |  Ludert, JE., et al. 2002. J Virol. 76: 6643-51. PMID: 12050377
  3. 转基因马铃薯中轮状病毒囊膜蛋白 VP6 的表达及其对小鼠的口服免疫原性。  |  Yu, J. and Langridge, W. 2003. Transgenic Res. 12: 163-9. PMID: 12739884
  4. 胰蛋白酶与轮状病毒的外壳相关联,并通过溶解外壳蛋白而被激活。  |  Benureau, Y., et al. 2005. J Gen Virol. 86: 3143-3151. PMID: 16227238
  5. 轮状病毒抗VP6分泌型免疫球蛋白A通过细胞内中和作用而非免疫排斥作用起到保护作用。  |  Corthésy, B., et al. 2006. J Virol. 80: 10692-9. PMID: 16956954
  6. 用重组外囊蛋白组装高传染性轮状病毒颗粒。  |  Trask, SD. and Dormitzer, PR. 2006. J Virol. 80: 11293-304. PMID: 16971442
  7. 在病毒周期结束时,通过耗尽内质网中螯合的 Ca2+ 使传染性轮状病毒颗粒在细胞内解体。  |  Ruiz, MC., et al. 2007. Virus Res. 130: 140-50. PMID: 17643542
  8. 轮状病毒感染会诱导 eIF2alpha 的磷酸化,但会阻止应激颗粒的形成。  |  Montero, H., et al. 2008. J Virol. 82: 1496-504. PMID: 18032499
  9. 轮状病毒颗粒同心层内的几何错配:病毒颗粒转录活动的潜在调控开关。  |  Libersou, S., et al. 2008. J Virol. 82: 2844-52. PMID: 18184711
  10. 转基因植物中产生的轮状病毒衣壳蛋白的免疫原性和病毒样颗粒形成。  |  Yang, Y., et al. 2011. Sci China Life Sci. 54: 82-9. PMID: 21104033
  11. 轮状病毒荚膜 VP6 蛋白在联合疫苗中作为诺如病毒样颗粒的体内佐剂。  |  Blazevic, V., et al. 2016. Hum Vaccin Immunother. 12: 740-8. PMID: 26467630
  12. 当与诺如病毒病毒样颗粒共同递送时,轮状病毒衣壳蛋白VP6的管状和球状纳米结构可作为局部佐剂。  |  Malm, M., et al. 2017. Clin Exp Immunol. 189: 331-341. PMID: 28407442

订购信息

产品名称产品编号规格价格数量收藏夹

Rotavirus capsid 抗体 (2B4)

sc-101363
50 µg/0.5 ml
$316.00

Hello, I´m not sure how to store this antibody. Datasheet says -20 °C and the vial says Store at 4°C. Which one is correct?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2022-09-22

I suppose this Ab works only for rotavirus group A. Does it reacts against porcine rotavirus A as well? Are there possible crossreactions to rotavirus group B and C?

Asked by: faun365
Thank you for your question. You can find information on the reactivity of Rotavirus capsid Antibody (2B4): sc-101363 in porcine tissue in PMID 30249258. For further information please contact technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Hello, I write to ask an information about this antibody we bought recently... I have to performed an IHC-(P) on intestinal tissues...What kind of proteolitic pretratment is more suitable to unmask VP6 rotavirus protein? Trypsin? Thank you in advance! 

Asked by: Chiara71
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval when using this antibody on formalin-fixed paraffin-embedded tissue sections. A link to our recommended protocol on our website is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-07-19

Can sc-101363: Rotavirus capsid (2B4) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-101363: Rotavirus capsid (2B4) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Reliable Antibody for WBWorks well in WB, strong clear signal with no background.
Date published: 2017-01-25
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Rotavirus capsid 抗体 (2B4) is rated 4.5 out of 5 by 2.
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