RNF146 Double Nickase Plasmid (h): sc-417152-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF146 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNF146 Double Nickase Plasmid (h) and RNF146 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RNF146. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF146 Double Nickase Plasmid (h) | sc-417152-NIC | 20 µg | $410.00 | |||
RNF146 Double Nickase Plasmid (h2) | sc-417152-NIC-2 | 20 µg | $410.00 |
RNF146 encodes an E3 ubiquitin ligase (also known as Iduna) that recognizes poly(ADP-ribose) and couples PARP-dependent signals to ubiquitin-mediated proteasomal turnover. It participates in DNA damage response and replication stress pathways by promoting degradation of PARylated substrates such as axin, thereby intersecting with Wnt/β-catenin signaling and cellular homeostasis. Through regulation of PARylation-linked cell death programs and genome maintenance, RNF146 is studied in contexts of cancer-associated pathway remodeling and neurodegeneration-related stress responses. Its activity connects PARP signaling, ubiquitin-proteasome dynamics, and transcriptional programs that influence proliferation and survival.
RNF146 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RNF146 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RNF146. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RNF146 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RNF146-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.