RIOK2 Double Nickase Plasmid (m): sc-426346-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RIOK2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RIOK2 Double Nickase Plasmid (m) and RIOK2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Riok2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RIOK2 Double Nickase Plasmid (m) | sc-426346-NIC | 20 µg | $410.00 | |||
RIOK2 Double Nickase Plasmid (m2) | sc-426346-NIC-2 | 20 µg | $410.00 |
Riok2 encodes RIOK2, an atypical serine/threonine kinase that functions as an essential assembly factor in late cytoplasmic maturation of the 40S ribosomal subunit. RIOK2 participates in ribosome biogenesis by coordinating pre-rRNA processing and quality control with the release and recycling of pre-40S factors, linking translation capacity to cell growth and proliferation programs. Because ribosome production is tightly integrated with cell cycle regulation and cellular stress responses, perturbation of RIOK2 can alter proteostasis and global translational output. Dysregulation of ribosome biogenesis pathways is broadly implicated in developmental abnormalities and proliferative disease biology, making RIOK2 a useful node for mechanistic studies of growth control.
RIOK2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Riok2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Riok2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Riok2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Riok2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.