RGC32 CRISPR/Cas9 KO Plasmid (h): sc-404843
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RGC32 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RGC32 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RGC32 CRISPR/Cas9 KO Plasmid (h) | sc-404843 | 20 µg | $397.00 |
Overview
RGCC encodes RGC32 (response gene to complement 32), a cell cycle–associated protein induced by complement activation and diverse stress signals. RGC32 participates in regulation of G2/M progression and proliferation by modulating cyclin-dependent signaling and interacting with kinase pathways implicated in checkpoint control. It has also been linked to inflammatory and vascular biology through effects on endothelial and smooth muscle cell activation, migration, and extracellular matrix remodeling. Dysregulated RGC32 expression has been reported across multiple pathological contexts, including tumor-associated proliferation and fibrosis-related tissue remodeling, supporting its use as a mechanistic node for studying growth control and microenvironmental responses.
RGC32 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RGCC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RGCC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RGCC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RGC32 protein expression.
This CRISPR knockout system enables efficient generation of RGCC-deficient cell models for investigation of RGC32 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RGCC exon(s) critical for RGC32 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RGCC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RGC32 CRISPR/Cas9 KO Plasmid (h) and RGC32 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RGCC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RGC32 HDR Plasmid (h) and RGC32 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RGCC homology arms to support homology-directed repair at defined RGCC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.