RFLAT-1 CRISPR/Cas9 KO Plasmid (h): sc-404618

Overview

KLF13 encodes the transcription factor RFLAT-1, a Krüppel-like zinc finger protein that binds GC-rich promoter elements to modulate gene expression programs controlling lymphocyte activation and inflammatory signaling. RFLAT-1 has been linked to regulation of chemokine and cytokine transcription and can interface with pathways such as NF-κB-dependent transcriptional responses, shaping immune-cell differentiation and activation states. Dysregulated KLF13/RFLAT-1 activity has been studied in contexts of immune-mediated pathology and altered inflammatory gene signatures, as well as in transcriptional networks that contribute to aberrant proliferation and survival in disease-relevant cell types. These properties make KLF13 a useful node for dissecting stimulus-responsive transcriptional regulation and immune gene circuitry in human model systems.

RFLAT-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KLF13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KLF13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KLF13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RFLAT-1 protein expression.

This CRISPR knockout system enables efficient generation of KLF13-deficient cell models for investigation of RFLAT-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting KLF13 exon(s) critical for RFLAT-1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple KLF13 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by RFLAT-1 CRISPR/Cas9 KO Plasmid (h) and RFLAT-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KLF13 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by RFLAT-1 HDR Plasmid (h) and RFLAT-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KLF13 homology arms to support homology-directed repair at defined KLF13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.