Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RecQL1 Double Nickase Plasmid (h): sc-402845-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RecQL1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RecQL1 Double Nickase Plasmid (h) and RecQL1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RECQL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RecQL1 Antibody (A-9): sc-166388
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RecQL1 Double Nickase Plasmid (h) | sc-402845-NIC | 20 µg | $410.00 | |||
RecQL1 Double Nickase Plasmid (h2) | sc-402845-NIC-2 | 20 µg | $410.00 |
Human RECQL encodes RecQL1, a 3′–5′ DNA helicase of the RecQ family that safeguards genome stability during DNA replication and repair. RecQL1 participates in replication fork restart, resolution of stalled or regressed forks, and recombination-associated intermediates, linking it to core DNA damage response processes. Through these activities it supports accurate sister chromatid exchange control and maintenance of chromosomal integrity under genotoxic stress. Altered RECQL/RecQL1 function has been associated with elevated replication stress and genomic instability phenotypes relevant to cancer biology and DNA repair–defect research.
RecQL1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RECQL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RECQL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RECQL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RECQL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.