Rbx2 Antibody (G-8): sc-166554

Rbx2 Antibody (G-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Rbx2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rbx2 (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rbx2, also known as ROC2, plays a crucial role in the ubiquitin-proteasome pathway, functioning as a RING finger protein that facilitates ubiquitin transfer to target substrates, thereby regulating protein degradation and cellular homeostasis. This process is vital for maintaining proper cellular function, as Rbx2 helps control levels of key regulatory proteins involved in cell cycle progression, signal transduction, and stress responses. Rbx2 is closely related to Rbx1 (ROC1), which is a component of the Skp1-Cdc53-F-box protein (SCF) ubiquitin ligase complex, and both proteins are essential for ubiquitination of various substrates, including cyclins and transcription factors. Rbx2′s ability to interact with other proteins in the ubiquitin ligase complex underscores its importance in cellular regulation and highlights its potential as a target for therapeutic intervention in diseases characterized by dysregulated protein degradation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rbx2 Antibody (G-8) References:

1. Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase.  |  Kamura, T., et al. 1999. Science. 284: 657-61. PMID: 10213691
2. Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha.  |  Tan, P., et al. 1999. Mol Cell. 3: 527-33. PMID: 10230406
3. ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.  |  Ohta, T., et al. 1999. Mol Cell. 3: 535-41. PMID: 10230407
4. Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34.  |  Seol, JH., et al. 1999. Genes Dev. 13: 1614-26. PMID: 10385629
5. Identification of the von Hippel-lindau tumor-suppressor protein as part of an active E3 ubiquitin ligase complex.  |  Iwai, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 12436-41. PMID: 10535940
6. Both Rbx1 and Rbx2 exhibit a functional role in the HIV-1 Vif-Cullin5 E3 ligase complex in vitro.  |  Wang, X., et al. 2015. Biochem Biophys Res Commun. 461: 624-9. PMID: 25912140
7. SAG/Rbx2-Dependent Neddylation Regulates T-Cell Responses.  |  Mathewson, ND., et al. 2016. Am J Pathol. 186: 2679-91. PMID: 27543965
8. RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion.  |  Fairchild, CL., et al. 2018. Development. 145: PMID: 29361558
9. Comparative Analysis of cul5 and rbx2 Expression in the Developing and Adult Murine Brain and Their Essentiality During Mouse Embryogenesis.  |  Hino, K., et al. 2018. Dev Dyn. 247: 1227-1236. PMID: 30269386
10. Sag/Rbx2 Partial Inactivation Sensitizes Mice to Radiation and Radiation-Induced Tumorigenesis1.  |  Sun, Y., et al. 2023. Radiat Res. 199: 273-282. PMID: 36745565
11. Advancements and perspectives of RBX2 as a molecular hallmark in cancer.  |  Tao, Y., et al. 2024. Gene. 892: 147864. PMID: 37820940
12. Ubiquitin Ligase RBX2/SAG Regulates Mitochondrial Ubiquitination and Mitophagy.  |  Wang, W., et al. 2024. Circ Res. 135: e39-e56. PMID: 38873758

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rbx2 Antibody (G-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Rbx2 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rbx2 Antibody (G-8) Specifications

Alternate Product Name: Rbx2

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 2-16

Epitope Target: Rbx2

Epitope Species: human

Product Reactivity/Detection: Rbx2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Rbx2 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin