RBM47 CRISPR/Cas9 KO Plasmid (m): sc-434185
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBM47 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RBM47 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBM47 CRISPR/Cas9 KO Plasmid (m) | sc-434185 | 20 µg | $397.00 |
Overview
Rbm47 encodes RBM47, an RNA-binding protein that associates with transcripts to influence post-transcriptional gene regulation, including mRNA stability, splicing, and RNA editing outcomes. RBM47 has been linked to the APOBEC1-dependent cytidine-to-uridine (C-to-U) RNA editing pathway, contributing to transcript diversification in epithelial and gastrointestinal contexts. Through modulation of RNA processing programs, RBM47 can impact cell-state decisions such as differentiation and epithelial integrity, with reported connections to dysregulated gene expression signatures in cancer- and inflammation-relevant settings. In mouse systems, Rbm47 perturbation provides a tractable route to dissect RNA-centric control of signaling networks and tissue homeostasis.
RBM47 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rbm47 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rbm47 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rbm47 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RBM47 protein expression.
This CRISPR knockout system enables efficient generation of Rbm47-deficient cell models for investigation of RBM47 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rbm47 exon(s) critical for RBM47 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rbm47 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RBM47 CRISPR/Cas9 KO Plasmid (m) and RBM47 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rbm47 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RBM47 HDR Plasmid (m) and RBM47 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rbm47 homology arms to support homology-directed repair at defined Rbm47 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.