Ral BP-1 CRISPR/Cas9 KO Plasmid (h): sc-402752
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ral BP-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ral BP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ral BP-1 Antibody (H-10): sc-48337
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ral BP-1 CRISPR/Cas9 KO Plasmid (h) | sc-402752 | 20 µg | $397.00 |
Overview
RALBP1 encodes Ral BP-1 (also known as RLIP76), a multifunctional RalA/RalB effector that links small GTPase signaling to endocytosis, cytoskeletal remodeling, and membrane trafficking. Ral BP-1 interacts with components of clathrin-dependent internalization and adaptor complexes to influence receptor turnover and vesicle dynamics, while also contributing to stress-response networks through ATP-dependent transport of glutathione conjugates and xenobiotic metabolites. Through these activities, RALBP1 impacts processes such as cell migration, cell cycle progression, and cellular resistance to oxidative and electrophilic stress. Dysregulated RALBP1 expression or signaling has been reported in multiple cancer contexts and is frequently studied in relation to invasion, metastasis-associated pathways, and cellular stress tolerance.
Ral BP-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RALBP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RALBP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RALBP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ral BP-1 protein expression.
This CRISPR knockout system enables efficient generation of RALBP1-deficient cell models for investigation of Ral BP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RALBP1 exon(s) critical for Ral BP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RALBP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ral BP-1 CRISPR/Cas9 KO Plasmid (h) and Ral BP-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RALBP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ral BP-1 HDR Plasmid (h) and Ral BP-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RALBP1 homology arms to support homology-directed repair at defined RALBP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.