Date published: 2025-9-9
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BRAF Antibody (F-3) - Western Blotting - Image 12983
Raf-B 抗体 (F-3): sc-55522. A-431 (A), CCRF-CEM (B), HL-60 (C), THP-1 (D), Hep G2 (E) 和 NIH/3T3 (F) 全细胞裂解液中 Raf-B 表达的 Western 印迹分析.
Raf-B 抗体 (F-3): sc-55522. A-431 (A), CCRF-CEM (B), HL-60 (C), THP-1 (D), Hep G2 (E) 和 NIH/3T3 (F) 全细胞裂解液中 Raf-B 表达的 Western 印迹分析.

BRAF 抗体 (F-3): sc-55522

4.9(10)
寫評論提問

说明书
  • BRAF抗体(F-3)是小鼠单克隆IgG2a κ, 在16篇文献中引用，规格为200 µg/ml
  • 免疫human来源Raf-B位于N-terminus 的12-156氨基酸
  • 抗-BRAF 抗体 (F-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Raf-B
  • 抗BRAF抗体（F-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BRAF (F-3): sc-55522的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 BRAF 抗体 (F-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与BRAF 抗体 (F-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Raf 抗体包括 A-Raf, Raf-1 和 Raf-B

Raf-B 抗体（F-3）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附试验（ELISA）等应用检测小鼠、大鼠和人样本中的 Raf-B。Raf-B (F-3) 抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Raf-B 又称 BRAF，是有丝分裂原激活蛋白激酶（MAPK）信号通路的关键组成部分，在调节细胞生长和分化方面发挥着至关重要的作用。这条途径对于将信号从细胞表面受体传递到细胞核，最终影响细胞对生长因子和压力的反应至关重要。Raf-B 主要位于细胞质中，在细胞质中，Raf-B 与其他信号分子（包括 ERK/MAP 激酶和核糖体 S6 激酶）相互作用，传播生长信号。值得注意的是，Raf-B 的表达具有高度局限性，在大脑和睾丸中的表达水平最高，这表明其在这些组织中具有特殊功能。Raf-B 的调控至关重要，因为不受调控的 Raf-B 与致癌转化有关，这使得 Raf-B 成为癌症研究和治疗干预的一个重要靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

BRAF 抗体 (F-3)

sc-55522
200 µg/ml
$316.00

BRAF (F-3): m-IgG Fc BP-HRP 套装

sc-525633
200 µg Ab; 10 µg BP
$354.00

BRAF (F-3): m-IgG2a BP-HRP 套装

sc-546345
200 µg Ab; 10 µg BP
$354.00

BRAF 抗体 (F-3) AC

sc-55522 AC
500 µg/ml, 25% agarose
$416.00

BRAF 抗体 (F-3) HRP

sc-55522 HRP
200 µg/ml
$316.00

BRAF 抗体 (F-3) FITC

sc-55522 FITC
200 µg/ml
$330.00

BRAF 抗体 (F-3) PE

sc-55522 PE
200 µg/ml
$343.00

BRAF 抗体 (F-3) Alexa Fluor® 488

sc-55522 AF488
200 µg/ml
$357.00

BRAF 抗体 (F-3) Alexa Fluor® 546

sc-55522 AF546
200 µg/ml
$357.00

BRAF 抗体 (F-3) Alexa Fluor® 594

sc-55522 AF594
200 µg/ml
$357.00

BRAF 抗体 (F-3) Alexa Fluor® 647

sc-55522 AF647
200 µg/ml
$357.00

BRAF 抗体 (F-3) Alexa Fluor® 680

sc-55522 AF680
200 µg/ml
$357.00

BRAF 抗体 (F-3) Alexa Fluor® 790

sc-55522 AF790
200 µg/ml
$357.00

I want to detect the level of Braf mutant protein (e.g. V600E) of mouse or human origin, can I use this sc-55522 antibody in wb experiments? s55522

Asked by: Cathyu
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2023-07-03

Have anyone used this antibody for immunohistochemistry for paraffin embedded sections

Asked by: Shanuja
Thank you for your question. Raf-B Antibody (F-3): sc-55522 is recommended for detection of Raf-B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. As of now there are no publications that describe the use of this antibody in IHC(P) applications. However we have established this antibody for those applications using mouse, rat, and human tissue samples with our standard protocols with reliable results and guarantee the efficacy of this antibody for the uses listed above. Feel free to contact our technical service directly if you require additional information.
Answered by: Tech Support Europe
Date published: 2019-05-14

I am using Raf-B (F-3): sc-55522 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excelent for western blotGives identical signal with anti Braf F7 antibody.
Date published: 2020-09-18
Rated 4 out of 5 by from Good for WBI use it for WB (1:1000), it shows a specific band.
Date published: 2020-02-13
Rated 5 out of 5 by from Great on WBThis antibody produced great and strong bands on WB (1:200 in 5% milk).
Date published: 2018-10-16
Rated 5 out of 5 by from Anticorps Super specifique !J'ai testé cet anticorps en WB sur une lignée de mélanome et il marche super bien ! Dilution 1/500
Date published: 2017-12-21
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of Raf-B expression in A-431, CCRF-CEM, HL-60, THP-1, Hep G2 and NIH/3T3 whole cell lysates.
Date published: 2017-01-27
Rated 5 out of 5 by from good release data.In mouse epidermis melanocytes WB good release data. WB A-431, CCRF-CEM, HL-60, THP-1 single and strong band, Hep G2 and NIH/3T3 cell lysate.
Date published: 2017-01-11
Rated 5 out of 5 by from Strong band in WB in human MCF7 breast cancerStrong band in WB in human MCF7 breast cancer cells. -SCBT Publication Review
Date published: 2015-04-20
Rated 5 out of 5 by from Good publishable data in WB in mouse epidermalGood publishable data in WB in mouse epidermal melanocyte. -SCBT Publication Review
Date published: 2015-03-30
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BRAF 抗体 (F-3) is rated 4.9 out of 5 by 10.
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