Date published: 2025-9-7

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BRAF 抗体 (F-7): sc-5284

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说明书
  • BRAF 抗体 F-7 是小鼠单克隆 IgG2a κ,BRAF抗体, 在295篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Raf-B的氨基酸12-156
  • BRAF 抗体 (F-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Raf-B
  • 抗BRAF抗体(F-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BRAF (F-7): sc-5284免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 BRAF 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与BRAF 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Raf-B抗体(F-7)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过WB、IP、IF、IHCP和ELISA检测小鼠、大鼠和人源的Raf-B。Raf-B抗体(F-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Raf-B 单克隆抗体(F-7)在细胞信号传导途径的调节中发挥着关键作用,特别是在细胞生长、分化及存活所必需的促分裂原活化蛋白激酶(MAPK)级联反应中。Raf-B(F-7)抗体是生长因子和激素向细胞核传递信号的关键介质,影响细胞对外界刺激的反应。Raf-B主要表达于大脑和睾丸,表明可能参与这些组织内的特定生理过程。调节Raf-B活性至关重要,因为异常信号可导致细胞无序增殖,并可能引发多种癌症。Raf-B(F-7)单克隆抗体使研究人员能够深入了解Raf-B在不同生物学环境下的功能动态,从而增进对其在健康和疾病中的作用的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BRAF 抗体 (F-7) 参考文献:

  1. B-RAF在黑色素瘤中的作用。  |  Gray-Schopfer, VC., et al. 2005. Cancer Metastasis Rev. 24: 165-83. PMID: 15785879
  2. raf家族原癌基因在正常小鼠组织中的表达。  |  Storm, SM., et al. 1990. Oncogene. 5: 345-51. PMID: 1690378
  3. B-Raf是治疗黑色素瘤和其他恶性肿瘤的理想靶点吗?  |  Madhunapantula, SV. and Robertson, GP. 2008. Cancer Res. 68: 5-8. PMID: 18172288
  4. 减数分裂和有丝分裂细胞周期控制中的蛋白激酶级联。  |  Pelech, SL., et al. 1990. Biochem Cell Biol. 68: 1297-330. PMID: 2085430
  5. c-raf-1 的突变激活和最小转化序列的定义。  |  Heidecker, G., et al. 1990. Mol Cell Biol. 10: 2503-12. PMID: 2188091
  6. c-raf 原癌基因在人类造血细胞和细胞系中的表达。  |  Sariban, E., et al. 1987. Blood. 69: 1437-40. PMID: 2436688
  7. 从膜到细胞质的信号转导:生长因子和膜结合的癌基因产物会增加 Raf-1 磷酸化和相关蛋白激酶的活性。  |  Morrison, DK., et al. 1988. Proc Natl Acad Sci U S A. 85: 8855-9. PMID: 3057494
  8. 在小鼠乳腺癌模型中,B-Raf 缺乏会影响肿瘤的发生和发展。  |  Köhler, M., et al. 2019. Oncogene. 38: 1324-1339. PMID: 30659267
  9. 关于B-RafV600E突变作为癌症治疗潜在靶点的系统综述。  |  Chavda, J. and Bhatt, H. 2020. Eur J Med Chem. 206: 112675. PMID: 32798788
  10. 胰岛素刺激的微管相关蛋白激酶在体内被酪氨酸和苏氨酸磷酸化。  |  Ray, LB. and Sturgill, TW. 1988. Proc Natl Acad Sci U S A. 85: 3753-7. PMID: 3287375
  11. 小鼠 A-raf 的特征,这是一种与 v-raf 癌基因相关的新的癌基因。  |  Huleihel, M., et al. 1986. Mol Cell Biol. 6: 2655-62. PMID: 3491291
  12. 由逆转录病毒转导的独特癌基因 v-raf 的结构和生物活性。  |  Rapp, UR., et al. 1983. Proc Natl Acad Sci U S A. 80: 4218-22. PMID: 6308607

订购信息

产品名称产品编号规格价格数量收藏夹

BRAF 抗体 (F-7)

sc-5284
200 µg/ml
$316.00

BRAF (F-7): m-IgG Fc BP-HRP 套装

sc-528144
200 µg Ab; 10 µg BP
$354.00

BRAF (F-7): m-IgGκ BP-HRP 套装

sc-520451
200 µg Ab, 40 µg BP
$354.00

BRAF (F-7): m-IgG2a BP-HRP 套装

sc-547032
200 µg Ab; 10 µg BP
$354.00

BRAF 抗体 (F-7) AC

sc-5284 AC
500 µg/ml, 25% agarose
$416.00

BRAF 抗体 (F-7) HRP

sc-5284 HRP
200 µg/ml
$316.00

BRAF 抗体 (F-7) FITC

sc-5284 FITC
200 µg/ml
$330.00

BRAF 抗体 (F-7) PE

sc-5284 PE
200 µg/ml
$343.00

BRAF 抗体 (F-7) Alexa Fluor® 488

sc-5284 AF488
200 µg/ml
$357.00

BRAF 抗体 (F-7) Alexa Fluor® 546

sc-5284 AF546
200 µg/ml
$357.00

BRAF 抗体 (F-7) Alexa Fluor® 594

sc-5284 AF594
200 µg/ml
$357.00

BRAF 抗体 (F-7) Alexa Fluor® 647

sc-5284 AF647
200 µg/ml
$357.00

BRAF 抗体 (F-7) Alexa Fluor® 680

sc-5284 AF680
200 µg/ml
$357.00

BRAF 抗体 (F-7) Alexa Fluor® 790

sc-5284 AF790
200 µg/ml
$357.00

I plan on doing native IP using this antibody. what should be my control?

Asked by: Ananya
Thank you for your question. https://www.scbt.com/p/normal-mouse-igg2a
Answered by: BlakeJ
Date published: 2025-07-10

Why do I get double bands when using Raf-B Antibody (F-7)?

Asked by: Lucia12
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Service SH
Date published: 2021-12-21

Our anti-BRAF sc 5284 were frozen at -20 degrees celsius instead of +4degrees celsius, during 12hours (by mistake), remain functionnal?

Asked by: SaraFao
Thank you for your question. We do not recommend freezing our antibodies because they contain gelatin in the PBS storage buffer, which upon freezing and thawing can sequester IgG out of solution and effect antibody performance. In your case, if the antibody was only frozen for a short amount of time, we would still encourage you to test it to determine if it is still working. It may or may not still be functional.
Answered by: Technical Support
Date published: 2017-02-02

What is the best Raf-B + control lysate for rat samples?

Asked by: Professor Griffin
Thank you for your question. We have positive Western blot data using a rat cell line, PC-12 whole cell lysate: sc-2250, with our Raf-B Antibody (F-7): sc-5284.
Answered by: Technical Support
Date published: 2016-12-20
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Rated 5 out of 5 by from This antibody works very well for WBThis antibody works very well for Western Blot (mouse lung tissue)
Date published: 2023-06-20
Rated 4 out of 5 by from Immunoblotting of B-RAFWe have tested the anti-B-RAF antibody (sc-5284) from Santa Cruz Biotechnology by immunoblotting. We have obtained very good results in human cell lines.
Date published: 2022-09-08
Rated 5 out of 5 by from クリアなバンド膵癌細胞株のタンパクで、データーシートにあるように1:200の希釈で行ったが、もう少し倍率を上げても、十分バンドを確認できるようだ。
Date published: 2021-12-13
Rated 1 out of 5 by from WBでバンドできないラットのサンプルですが、濃度は1:200から1:500までバンドできないです。ロットによってできそうですが
Date published: 2021-09-18
Rated 5 out of 5 by from Great antibody for WBThis primary antibody worked very well in Western blot.
Date published: 2021-03-31
Rated 5 out of 5 by from Good for multiplex WesternBRAF antibody works well at 1:1000, specifically detects BRAF and was used to confirm CRISPR deletion. Can multiplex with Rabbit antibodies
Date published: 2021-02-16
Rated 5 out of 5 by from Review of BRAF(Sc-5284) antibodyThe BRAF(Sc-5284)antibody was used for western blot analysis. The product origin is mouse. The product was very good enough to get a clear band. I would strongly recommend this antibody for other users too.
Date published: 2020-08-20
Rated 5 out of 5 by from BRAFAplin lab used antibody for western blotting while experimenting.
Date published: 2019-02-06
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BRAF 抗体 (F-7) is rated 4.7 out of 5 by 23.
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