Rad51B Antibody (D-12): sc-377191
- Rad51B Antibody (D-12) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 141-255 mapping within an internal region of Rad51B of human origin
- recommended for detection of Rad51B of human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-377191 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Rad51B Antibody (D-12) for WB applications. This reagent is now offered in a bundle with Rad51B Antibody (D-12) (see ordering information below).
Rad51B Antibody (D-12) is a mouse monoclonal IgG2a kappa light chain antibody engineered to recognize an internal epitope spanning amino acids 141-255 of the human Rad51B protein, ensuring precise detection in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rad51B, also called R51H2, mREC2, and Rad51l1, is a crucial member of the Rad51 family involved in the homologous recombination repair pathway, which is essential for the accurate repair of DNA double-strand breaks and the maintenance of genomic stability. Structurally, Rad51B forms complexes with other Rad51 paralogs, facilitating the search for homology and strand pairing during DNA repair processes. Post-translational modifications of Rad51B, including phosphorylation and ubiquitination, play vital roles in regulating activity, interactions, and localization within the cell. These modifications are critical for controlling Rad51B′s involvement in DNA repair mechanisms, ensuring timely and accurate response to DNA damage, and preventing genomic instability that can lead to mutations and cancer development. The precise regulation of Rad51B through these modifications underscores importance in cellular defense against genetic aberrations. Rad51B (D-12) monoclonal antibody does not cross-react with other Rad51 family members, providing high specificity and reliability for researchers studying DNA repair pathways, cancer biology, and genomic integrity. Available in both non-conjugated form and various conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, anti-Rad51B antibody (D-12) offers versatile applications for diverse experimental needs. By targeting functionally and post-translationally regulated regions of Rad51B, anti-Rad51B antibody (D-12) serves as an invaluable tool for elucidating molecular mechanisms underlying DNA repair, maintenance of genomic stability, and cellular responses to DNA damage, thereby advancing our understanding of cancer development and potential therapeutic interventions.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rad51B Antibody (D-12) | sc-377191 | 200 µg/ml | $322.00 | |||
Rad51B Antibody (D-12): m-IgG Fc BP-HRP Bundle | sc-540700 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rad51B Antibody (D-12) X | sc-377191 X | 200 µg/0.1 ml | $322.00 |