Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RABAPTIN-5 CRISPR/Cas9 KO Plasmid (h): sc-403303
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RABAPTIN-5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RABAPTIN-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RABAPTIN-5 Antibody (B-8): sc-271069
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RABAPTIN-5 CRISPR/Cas9 KO Plasmid (h) | sc-403303 | 20 µg | $397.00 |
Overview
RABEP1 encodes RABAPTIN-5, a Rab5 effector that coordinates early endocytic trafficking by promoting vesicle tethering and fusion events on early endosomes. Through interactions with Rab5-family GTPases and endosomal sorting machinery, RABAPTIN-5 helps regulate receptor internalization, cargo recycling versus degradation decisions, and endosome maturation. These processes influence signaling output from surface receptors and the turnover of membrane proteins, linking RABEP1 function to pathways controlling growth factor signaling, nutrient uptake, and membrane homeostasis. Dysregulation of endocytic control and Rab5-dependent trafficking has been associated with altered signaling dynamics and cellular phenotypes observed in cancer biology and neurodegeneration-relevant models.
RABAPTIN-5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RABEP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RABEP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RABEP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RABAPTIN-5 protein expression.
This CRISPR knockout system enables efficient generation of RABEP1-deficient cell models for investigation of RABAPTIN-5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RABEP1 exon(s) critical for RABAPTIN-5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RABEP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RABAPTIN-5 CRISPR/Cas9 KO Plasmid (h) and RABAPTIN-5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RABEP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RABAPTIN-5 HDR Plasmid (h) and RABAPTIN-5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RABEP1 homology arms to support homology-directed repair at defined RABEP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.